Abstract
Introduction: This study was to evaluate the in vivo distribution of intravenously transplanted bone marrow-derived mesenchymal stem cells (BMSCs) in an acute brain trauma model by {sup 111}In-tropolone labeling. Methods: Rat BMSCs were labeled with 37 MBq {sup 111}In-tropolone. Their labeling efficiency and in vitro retention rate were measured. The viability and proliferation of labeled BMSCs were evaluated for 14 days after labeling. The biodistribution of {sup 111}In-labeled BMSCs in trauma models was compared with those of sham-operated rats and normal rats on gamma camera images. The migration of {sup 111}In-BMSCs to the traumatic brain was evaluated using confocal microscope. Results: The labeling efficiency of {sup 111}In-BMSCs was 66{+-}5%, and their retention rate was 85.3% at 1 h after labeling. There was no difference in the number of viable cells between {sup 111}In-BMSCs and controls at 48 h after labeling. However, the proliferation of {sup 111}In-BMSCs was inhibited after the third day of labeling, and it did not reach confluency. On gamma camera images, most of the {sup 111}In-BMSCs uptake was observed in the liver and spleen at the second day of injection. The brain uptake of {sup 111}In-BMSCs was detected prominently in trauma models (1.4%) than in sham-operated (0.5%)
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Yoon, Joon-Kee;
[1]
Institute for Neuroregeneration and Stem Cell Research, Ajou University School of Medicine, Suwon (Korea, Republic of)];
Park, Bok-Nam;
[1]
Shim, Woo-Young;
[2]
Department of Molecular Science and Technology, Ajou University, Suwon (Korea, Republic of)];
Shin, Jin Young;
[2]
Department of Neurosurgery, Ajou University School of Medicine, Suwon (Korea, Republic of)];
Lee, Gwang;
[2]
Department of Molecular Science and Technology, Ajou University, Suwon (Korea, Republic of);
Brain Disease Research Center, Ajou University School of Medicine, Suwon (Korea, Republic of)];
Ahn, Young Hwan;
[2]
Department of Neurosurgery, Ajou University School of Medicine, Suwon (Korea, Republic of)], E-mail: yhahn@ajou.ac.kr
- Department of Nuclear Medicine and Molecular Imaging, Ajou University School of Medicine, Suwon (Korea, Republic of)
- Institute for Neuroregeneration and Stem Cell Research, Ajou University School of Medicine, Suwon (Korea, Republic of)
Citation Formats
Yoon, Joon-Kee, Institute for Neuroregeneration and Stem Cell Research, Ajou University School of Medicine, Suwon (Korea, Republic of)], Park, Bok-Nam, Shim, Woo-Young, Department of Molecular Science and Technology, Ajou University, Suwon (Korea, Republic of)], Shin, Jin Young, Department of Neurosurgery, Ajou University School of Medicine, Suwon (Korea, Republic of)], Lee, Gwang, Department of Molecular Science and Technology, Ajou University, Suwon (Korea, Republic of), Brain Disease Research Center, Ajou University School of Medicine, Suwon (Korea, Republic of)], Ahn, Young Hwan, and Department of Neurosurgery, Ajou University School of Medicine, Suwon (Korea, Republic of)], E-mail: yhahn@ajou.ac.kr.
In vivo tracking of {sup 111}In-labeled bone marrow mesenchymal stem cells in acute brain trauma model.
United Kingdom: N. p.,
2010.
Web.
doi:10.1016/j.nucmedbio.2009.12.001.
Yoon, Joon-Kee, Institute for Neuroregeneration and Stem Cell Research, Ajou University School of Medicine, Suwon (Korea, Republic of)], Park, Bok-Nam, Shim, Woo-Young, Department of Molecular Science and Technology, Ajou University, Suwon (Korea, Republic of)], Shin, Jin Young, Department of Neurosurgery, Ajou University School of Medicine, Suwon (Korea, Republic of)], Lee, Gwang, Department of Molecular Science and Technology, Ajou University, Suwon (Korea, Republic of), Brain Disease Research Center, Ajou University School of Medicine, Suwon (Korea, Republic of)], Ahn, Young Hwan, & Department of Neurosurgery, Ajou University School of Medicine, Suwon (Korea, Republic of)], E-mail: yhahn@ajou.ac.kr.
In vivo tracking of {sup 111}In-labeled bone marrow mesenchymal stem cells in acute brain trauma model.
United Kingdom.
https://doi.org/10.1016/j.nucmedbio.2009.12.001
Yoon, Joon-Kee, Institute for Neuroregeneration and Stem Cell Research, Ajou University School of Medicine, Suwon (Korea, Republic of)], Park, Bok-Nam, Shim, Woo-Young, Department of Molecular Science and Technology, Ajou University, Suwon (Korea, Republic of)], Shin, Jin Young, Department of Neurosurgery, Ajou University School of Medicine, Suwon (Korea, Republic of)], Lee, Gwang, Department of Molecular Science and Technology, Ajou University, Suwon (Korea, Republic of), Brain Disease Research Center, Ajou University School of Medicine, Suwon (Korea, Republic of)], Ahn, Young Hwan, and Department of Neurosurgery, Ajou University School of Medicine, Suwon (Korea, Republic of)], E-mail: yhahn@ajou.ac.kr.
2010.
"In vivo tracking of {sup 111}In-labeled bone marrow mesenchymal stem cells in acute brain trauma model."
United Kingdom.
https://doi.org/10.1016/j.nucmedbio.2009.12.001.
@misc{etde_21332962,
title = {In vivo tracking of {sup 111}In-labeled bone marrow mesenchymal stem cells in acute brain trauma model}
author = {Yoon, Joon-Kee, Institute for Neuroregeneration and Stem Cell Research, Ajou University School of Medicine, Suwon (Korea, Republic of)], Park, Bok-Nam, Shim, Woo-Young, Department of Molecular Science and Technology, Ajou University, Suwon (Korea, Republic of)], Shin, Jin Young, Department of Neurosurgery, Ajou University School of Medicine, Suwon (Korea, Republic of)], Lee, Gwang, Department of Molecular Science and Technology, Ajou University, Suwon (Korea, Republic of), Brain Disease Research Center, Ajou University School of Medicine, Suwon (Korea, Republic of)], Ahn, Young Hwan, and Department of Neurosurgery, Ajou University School of Medicine, Suwon (Korea, Republic of)], E-mail: yhahn@ajou.ac.kr}
abstractNote = {Introduction: This study was to evaluate the in vivo distribution of intravenously transplanted bone marrow-derived mesenchymal stem cells (BMSCs) in an acute brain trauma model by {sup 111}In-tropolone labeling. Methods: Rat BMSCs were labeled with 37 MBq {sup 111}In-tropolone. Their labeling efficiency and in vitro retention rate were measured. The viability and proliferation of labeled BMSCs were evaluated for 14 days after labeling. The biodistribution of {sup 111}In-labeled BMSCs in trauma models was compared with those of sham-operated rats and normal rats on gamma camera images. The migration of {sup 111}In-BMSCs to the traumatic brain was evaluated using confocal microscope. Results: The labeling efficiency of {sup 111}In-BMSCs was 66{+-}5%, and their retention rate was 85.3% at 1 h after labeling. There was no difference in the number of viable cells between {sup 111}In-BMSCs and controls at 48 h after labeling. However, the proliferation of {sup 111}In-BMSCs was inhibited after the third day of labeling, and it did not reach confluency. On gamma camera images, most of the {sup 111}In-BMSCs uptake was observed in the liver and spleen at the second day of injection. The brain uptake of {sup 111}In-BMSCs was detected prominently in trauma models (1.4%) than in sham-operated (0.5%) or normal rats (0.3%). Radiolabeled BMSCs were observed at the traumatic brain on the confocal microscope as they have a homing capacity, although its proliferation capacity was suppressed. Conclusion: Although growth inhibition by {sup 111}In-labeling need to be evaluated further prior to use in humans, {sup 111}In-labeled BMSCs are useful for the tracking of intravenously transplanted mesenchymal stem cells in brain disease models.}
doi = {10.1016/j.nucmedbio.2009.12.001}
journal = []
issue = {3}
volume = {37}
place = {United Kingdom}
year = {2010}
month = {Apr}
}
title = {In vivo tracking of {sup 111}In-labeled bone marrow mesenchymal stem cells in acute brain trauma model}
author = {Yoon, Joon-Kee, Institute for Neuroregeneration and Stem Cell Research, Ajou University School of Medicine, Suwon (Korea, Republic of)], Park, Bok-Nam, Shim, Woo-Young, Department of Molecular Science and Technology, Ajou University, Suwon (Korea, Republic of)], Shin, Jin Young, Department of Neurosurgery, Ajou University School of Medicine, Suwon (Korea, Republic of)], Lee, Gwang, Department of Molecular Science and Technology, Ajou University, Suwon (Korea, Republic of), Brain Disease Research Center, Ajou University School of Medicine, Suwon (Korea, Republic of)], Ahn, Young Hwan, and Department of Neurosurgery, Ajou University School of Medicine, Suwon (Korea, Republic of)], E-mail: yhahn@ajou.ac.kr}
abstractNote = {Introduction: This study was to evaluate the in vivo distribution of intravenously transplanted bone marrow-derived mesenchymal stem cells (BMSCs) in an acute brain trauma model by {sup 111}In-tropolone labeling. Methods: Rat BMSCs were labeled with 37 MBq {sup 111}In-tropolone. Their labeling efficiency and in vitro retention rate were measured. The viability and proliferation of labeled BMSCs were evaluated for 14 days after labeling. The biodistribution of {sup 111}In-labeled BMSCs in trauma models was compared with those of sham-operated rats and normal rats on gamma camera images. The migration of {sup 111}In-BMSCs to the traumatic brain was evaluated using confocal microscope. Results: The labeling efficiency of {sup 111}In-BMSCs was 66{+-}5%, and their retention rate was 85.3% at 1 h after labeling. There was no difference in the number of viable cells between {sup 111}In-BMSCs and controls at 48 h after labeling. However, the proliferation of {sup 111}In-BMSCs was inhibited after the third day of labeling, and it did not reach confluency. On gamma camera images, most of the {sup 111}In-BMSCs uptake was observed in the liver and spleen at the second day of injection. The brain uptake of {sup 111}In-BMSCs was detected prominently in trauma models (1.4%) than in sham-operated (0.5%) or normal rats (0.3%). Radiolabeled BMSCs were observed at the traumatic brain on the confocal microscope as they have a homing capacity, although its proliferation capacity was suppressed. Conclusion: Although growth inhibition by {sup 111}In-labeling need to be evaluated further prior to use in humans, {sup 111}In-labeled BMSCs are useful for the tracking of intravenously transplanted mesenchymal stem cells in brain disease models.}
doi = {10.1016/j.nucmedbio.2009.12.001}
journal = []
issue = {3}
volume = {37}
place = {United Kingdom}
year = {2010}
month = {Apr}
}