Abstract
The objective of the study was to evaluate state-of-the-art clinical PET/CT technology in performing static and dynamic imaging of several mice simultaneously. A mouse-sized phantom was imaged mimicking simultaneous imaging of three mice with computation of recovery coefficients (RCs) and spillover ratios (SORs). Fifteen mice harbouring abdominal or subcutaneous tumours were imaged on clinical PET/CT with point spread function (PSF) reconstruction after injection of [18F]fluorodeoxyglucose or [18F]fluorothymidine. Three of these mice were imaged alone and simultaneously at radial positions -5, 0 and 5 cm. The remaining 12 tumour-bearing mice were imaged in groups of 3 to establish the quantitative accuracy of PET data using ex vivo gamma counting as the reference. Finally, a dynamic scan was performed in three mice simultaneously after the injection of {sup 68}Ga-ethylenediaminetetraacetic acid (EDTA). For typical lesion sizes of 7-8 mm phantom experiments indicated RCs of 0.42 and 0.76 for ordered subsets expectation maximization (OSEM) and PSF reconstruction, respectively. For PSF reconstruction, SOR{sub air} and SOR{sub water} were 5.3 and 7.5%, respectively. A strong correlation (r {sup 2} = 0.97, p < 0.0001) between quantitative data obtained in mice imaged alone and simultaneously in a group of three was found following PSF reconstruction. The correlation
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Aide, Nicolas;
[1]
Caen University Hospital and Francois Baclesse Cancer Centre, PET Unit, Caen (France);
Peter MacCallum Cancer Centre, Centre for Molecular Imaging, East Melbourne (Australia);
Centre Francois Baclesse, Nuclear Medicine Department, Caen cedex 5 (France)];
Desmonts, Cedric;
Agostini, Denis;
Bardet, Stephane;
Bouvard, Gerard;
[2]
Beauregard, Jean-Mathieu;
Roselt, Peter;
Neels, Oliver;
[3]
Beyer, Thomas;
[4]
University Hospital Essen, Department of Nuclear Medicine, Essen (Germany);
University Hospital Bern, Institute of Nuclear Medicine, Bern (Switzerland)];
Kinross, Kathryn;
[3]
Peter MacCallum Cancer Centre, Sir Donald and Lady Trescowthick Laboratories, East Melbourne (Australia)];
Hicks, Rodney J;
[3]
University of Melbourne, The Department of Medicine, Parkville (Australia)]
- Francois Baclesse Cancer Centre and Caen University, Bioticla Team, EA1792, IFR 146 ICORE, GRECAN, Caen (France)
- Caen University Hospital and Francois Baclesse Cancer Centre, PET Unit, Caen (France)
- Peter MacCallum Cancer Centre, Centre for Molecular Imaging, East Melbourne (Australia)
- cmi-experts GmbH, Zurich (Switzerland)
Citation Formats
Aide, Nicolas, Caen University Hospital and Francois Baclesse Cancer Centre, PET Unit, Caen (France), Peter MacCallum Cancer Centre, Centre for Molecular Imaging, East Melbourne (Australia), Centre Francois Baclesse, Nuclear Medicine Department, Caen cedex 5 (France)], Desmonts, Cedric, Agostini, Denis, Bardet, Stephane, Bouvard, Gerard, Beauregard, Jean-Mathieu, Roselt, Peter, Neels, Oliver, Beyer, Thomas, University Hospital Essen, Department of Nuclear Medicine, Essen (Germany), University Hospital Bern, Institute of Nuclear Medicine, Bern (Switzerland)], Kinross, Kathryn, Peter MacCallum Cancer Centre, Sir Donald and Lady Trescowthick Laboratories, East Melbourne (Australia)], Hicks, Rodney J, and University of Melbourne, The Department of Medicine, Parkville (Australia)].
High throughput static and dynamic small animal imaging using clinical PET/CT: potential preclinical applications.
Germany: N. p.,
2010.
Web.
doi:10.1007/S00259-009-1352-1.
Aide, Nicolas, Caen University Hospital and Francois Baclesse Cancer Centre, PET Unit, Caen (France), Peter MacCallum Cancer Centre, Centre for Molecular Imaging, East Melbourne (Australia), Centre Francois Baclesse, Nuclear Medicine Department, Caen cedex 5 (France)], Desmonts, Cedric, Agostini, Denis, Bardet, Stephane, Bouvard, Gerard, Beauregard, Jean-Mathieu, Roselt, Peter, Neels, Oliver, Beyer, Thomas, University Hospital Essen, Department of Nuclear Medicine, Essen (Germany), University Hospital Bern, Institute of Nuclear Medicine, Bern (Switzerland)], Kinross, Kathryn, Peter MacCallum Cancer Centre, Sir Donald and Lady Trescowthick Laboratories, East Melbourne (Australia)], Hicks, Rodney J, & University of Melbourne, The Department of Medicine, Parkville (Australia)].
High throughput static and dynamic small animal imaging using clinical PET/CT: potential preclinical applications.
Germany.
https://doi.org/10.1007/S00259-009-1352-1
Aide, Nicolas, Caen University Hospital and Francois Baclesse Cancer Centre, PET Unit, Caen (France), Peter MacCallum Cancer Centre, Centre for Molecular Imaging, East Melbourne (Australia), Centre Francois Baclesse, Nuclear Medicine Department, Caen cedex 5 (France)], Desmonts, Cedric, Agostini, Denis, Bardet, Stephane, Bouvard, Gerard, Beauregard, Jean-Mathieu, Roselt, Peter, Neels, Oliver, Beyer, Thomas, University Hospital Essen, Department of Nuclear Medicine, Essen (Germany), University Hospital Bern, Institute of Nuclear Medicine, Bern (Switzerland)], Kinross, Kathryn, Peter MacCallum Cancer Centre, Sir Donald and Lady Trescowthick Laboratories, East Melbourne (Australia)], Hicks, Rodney J, and University of Melbourne, The Department of Medicine, Parkville (Australia)].
2010.
"High throughput static and dynamic small animal imaging using clinical PET/CT: potential preclinical applications."
Germany.
https://doi.org/10.1007/S00259-009-1352-1.
@misc{etde_21320723,
title = {High throughput static and dynamic small animal imaging using clinical PET/CT: potential preclinical applications}
author = {Aide, Nicolas, Caen University Hospital and Francois Baclesse Cancer Centre, PET Unit, Caen (France), Peter MacCallum Cancer Centre, Centre for Molecular Imaging, East Melbourne (Australia), Centre Francois Baclesse, Nuclear Medicine Department, Caen cedex 5 (France)], Desmonts, Cedric, Agostini, Denis, Bardet, Stephane, Bouvard, Gerard, Beauregard, Jean-Mathieu, Roselt, Peter, Neels, Oliver, Beyer, Thomas, University Hospital Essen, Department of Nuclear Medicine, Essen (Germany), University Hospital Bern, Institute of Nuclear Medicine, Bern (Switzerland)], Kinross, Kathryn, Peter MacCallum Cancer Centre, Sir Donald and Lady Trescowthick Laboratories, East Melbourne (Australia)], Hicks, Rodney J, and University of Melbourne, The Department of Medicine, Parkville (Australia)]}
abstractNote = {The objective of the study was to evaluate state-of-the-art clinical PET/CT technology in performing static and dynamic imaging of several mice simultaneously. A mouse-sized phantom was imaged mimicking simultaneous imaging of three mice with computation of recovery coefficients (RCs) and spillover ratios (SORs). Fifteen mice harbouring abdominal or subcutaneous tumours were imaged on clinical PET/CT with point spread function (PSF) reconstruction after injection of [18F]fluorodeoxyglucose or [18F]fluorothymidine. Three of these mice were imaged alone and simultaneously at radial positions -5, 0 and 5 cm. The remaining 12 tumour-bearing mice were imaged in groups of 3 to establish the quantitative accuracy of PET data using ex vivo gamma counting as the reference. Finally, a dynamic scan was performed in three mice simultaneously after the injection of {sup 68}Ga-ethylenediaminetetraacetic acid (EDTA). For typical lesion sizes of 7-8 mm phantom experiments indicated RCs of 0.42 and 0.76 for ordered subsets expectation maximization (OSEM) and PSF reconstruction, respectively. For PSF reconstruction, SOR{sub air} and SOR{sub water} were 5.3 and 7.5%, respectively. A strong correlation (r {sup 2} = 0.97, p < 0.0001) between quantitative data obtained in mice imaged alone and simultaneously in a group of three was found following PSF reconstruction. The correlation between ex vivo counting and PET/CT data was better with PSF reconstruction (r {sup 2} = 0.98; slope = 0.89, p < 0.0001) than without (r {sup 2} = 0.96; slope = 0.62, p < 0.001). Valid time-activity curves of the blood pool, kidneys and bladder could be derived from {sup 68}Ga-EDTA dynamic acquisition. New generation clinical PET/CT can be used for simultaneous imaging of multiple small animals in experiments requiring high throughput and where a dedicated small animal PET system is not available. (orig.)}
doi = {10.1007/S00259-009-1352-1}
journal = []
issue = {5}
volume = {37}
place = {Germany}
year = {2010}
month = {May}
}
title = {High throughput static and dynamic small animal imaging using clinical PET/CT: potential preclinical applications}
author = {Aide, Nicolas, Caen University Hospital and Francois Baclesse Cancer Centre, PET Unit, Caen (France), Peter MacCallum Cancer Centre, Centre for Molecular Imaging, East Melbourne (Australia), Centre Francois Baclesse, Nuclear Medicine Department, Caen cedex 5 (France)], Desmonts, Cedric, Agostini, Denis, Bardet, Stephane, Bouvard, Gerard, Beauregard, Jean-Mathieu, Roselt, Peter, Neels, Oliver, Beyer, Thomas, University Hospital Essen, Department of Nuclear Medicine, Essen (Germany), University Hospital Bern, Institute of Nuclear Medicine, Bern (Switzerland)], Kinross, Kathryn, Peter MacCallum Cancer Centre, Sir Donald and Lady Trescowthick Laboratories, East Melbourne (Australia)], Hicks, Rodney J, and University of Melbourne, The Department of Medicine, Parkville (Australia)]}
abstractNote = {The objective of the study was to evaluate state-of-the-art clinical PET/CT technology in performing static and dynamic imaging of several mice simultaneously. A mouse-sized phantom was imaged mimicking simultaneous imaging of three mice with computation of recovery coefficients (RCs) and spillover ratios (SORs). Fifteen mice harbouring abdominal or subcutaneous tumours were imaged on clinical PET/CT with point spread function (PSF) reconstruction after injection of [18F]fluorodeoxyglucose or [18F]fluorothymidine. Three of these mice were imaged alone and simultaneously at radial positions -5, 0 and 5 cm. The remaining 12 tumour-bearing mice were imaged in groups of 3 to establish the quantitative accuracy of PET data using ex vivo gamma counting as the reference. Finally, a dynamic scan was performed in three mice simultaneously after the injection of {sup 68}Ga-ethylenediaminetetraacetic acid (EDTA). For typical lesion sizes of 7-8 mm phantom experiments indicated RCs of 0.42 and 0.76 for ordered subsets expectation maximization (OSEM) and PSF reconstruction, respectively. For PSF reconstruction, SOR{sub air} and SOR{sub water} were 5.3 and 7.5%, respectively. A strong correlation (r {sup 2} = 0.97, p < 0.0001) between quantitative data obtained in mice imaged alone and simultaneously in a group of three was found following PSF reconstruction. The correlation between ex vivo counting and PET/CT data was better with PSF reconstruction (r {sup 2} = 0.98; slope = 0.89, p < 0.0001) than without (r {sup 2} = 0.96; slope = 0.62, p < 0.001). Valid time-activity curves of the blood pool, kidneys and bladder could be derived from {sup 68}Ga-EDTA dynamic acquisition. New generation clinical PET/CT can be used for simultaneous imaging of multiple small animals in experiments requiring high throughput and where a dedicated small animal PET system is not available. (orig.)}
doi = {10.1007/S00259-009-1352-1}
journal = []
issue = {5}
volume = {37}
place = {Germany}
year = {2010}
month = {May}
}