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The expression of Smad4 after radiation of electromagnetic pulses and apoptosis in spermatogenic cells in mouse

Abstract

The aim of the study was to investigate the relationships between apoptosis induced by radiation of electromagnetic pulses (EMP) and the expression of Smad4 in mouse spermatogenic cells. 40 adult Balb/c mice were used, and 20 were irradiated with whole-body 400kV/m EMP. The mice were sacrificed and specimens were harvested at 1, 7, 14, 21 and 28 days after the irradiation. Histological changes were observed through Hematoxylin-Eosin staining (H-E staining), the apoptosis of spermatogenic cells was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling method (TUNEL method) and the Smad4 expression was observed using immunohistochemistry SABC methods. Severe injuries were observed 1 day after the radiation and seminiferous epithelium was mostly recovered 28d after the radiation. The localization of smad4 was significantly different in EMP group compared to the control group, and the expression densities of smad4 decreased significantly at 7, 14 and 21d after irradiation (p<0.05). TUNEL assays demonstrated that there was a significant increase in the mean apoptotic index (AI) in irradiation groups than that of control groups (p<0.01). The results suggested that Smad4 and TGF-13/Smad signal pathway might play an important role in spermatogenic cells apoptosis induced by radiation of EMP. (authors)
Authors:
Xinxin, Ji; Wugang, Hou; Jie, Zhao; Yong, Zhao; Wei, Li; Yuanqiang, Zhang [1] 
  1. Department of Histology and Embryology, Fourth Military Medical Univ., Xi'an (China)
Publication Date:
Apr 15, 2007
Product Type:
Journal Article
Resource Relation:
Journal Name: Journal of Radiation Research and Radiation Processing; Journal Volume: 25; Journal Issue: 2; Other Information: 9 figs., 14 refs
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; ADULTS; APOPTOSIS; DENSITY; ELECTROMAGNETIC PULSES; EOSIN; EPITHELIUM; HEMATOXYLIN; IRRADIATION; LABELLING; MICE; RADIATION INJURIES; SIGNALS; SPERMATOZOA
OSTI ID:
21306218
Country of Origin:
China
Language:
Chinese
Other Identifying Numbers:
Journal ID: ISSN 1000-3436; FYYXEA; TRN: CN1000707049963
Submitting Site:
INIS
Size:
page(s) 124-128
Announcement Date:
Jun 24, 2010

Citation Formats

Xinxin, Ji, Wugang, Hou, Jie, Zhao, Yong, Zhao, Wei, Li, and Yuanqiang, Zhang. The expression of Smad4 after radiation of electromagnetic pulses and apoptosis in spermatogenic cells in mouse. China: N. p., 2007. Web.
Xinxin, Ji, Wugang, Hou, Jie, Zhao, Yong, Zhao, Wei, Li, &amp; Yuanqiang, Zhang. The expression of Smad4 after radiation of electromagnetic pulses and apoptosis in spermatogenic cells in mouse. China.
Xinxin, Ji, Wugang, Hou, Jie, Zhao, Yong, Zhao, Wei, Li, and Yuanqiang, Zhang. 2007. "The expression of Smad4 after radiation of electromagnetic pulses and apoptosis in spermatogenic cells in mouse." China.
@misc{etde_21306218,
title = {The expression of Smad4 after radiation of electromagnetic pulses and apoptosis in spermatogenic cells in mouse}
author = {Xinxin, Ji, Wugang, Hou, Jie, Zhao, Yong, Zhao, Wei, Li, and Yuanqiang, Zhang}
abstractNote = {The aim of the study was to investigate the relationships between apoptosis induced by radiation of electromagnetic pulses (EMP) and the expression of Smad4 in mouse spermatogenic cells. 40 adult Balb/c mice were used, and 20 were irradiated with whole-body 400kV/m EMP. The mice were sacrificed and specimens were harvested at 1, 7, 14, 21 and 28 days after the irradiation. Histological changes were observed through Hematoxylin-Eosin staining (H-E staining), the apoptosis of spermatogenic cells was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling method (TUNEL method) and the Smad4 expression was observed using immunohistochemistry SABC methods. Severe injuries were observed 1 day after the radiation and seminiferous epithelium was mostly recovered 28d after the radiation. The localization of smad4 was significantly different in EMP group compared to the control group, and the expression densities of smad4 decreased significantly at 7, 14 and 21d after irradiation (p<0.05). TUNEL assays demonstrated that there was a significant increase in the mean apoptotic index (AI) in irradiation groups than that of control groups (p<0.01). The results suggested that Smad4 and TGF-13/Smad signal pathway might play an important role in spermatogenic cells apoptosis induced by radiation of EMP. (authors)}
journal = []
issue = {2}
volume = {25}
place = {China}
year = {2007}
month = {Apr}
}