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Effect of essential oil from Cordia verbeancea on the fermentation of a high concentrate diet by using the in vitro gas production technique

Conference:

Abstract

Studies with plant secondary metabolites as rumen fermentation modifiers have increased as an attempt to reproduce the effects of ionophores. Cordia verbenacea D.C. is a Brazilian bush with antimicrobial properties attributed to its essential oil (EO). The objective of this experiment was to determine the effects of C. verbenacea EO on the ruminal fermentation by using an in vitro gas production system. Treatments were defined as: Control - without addition of monensin or EO; MON - addition of monensin (Sigma Aldrich Inc.) at 3 {mu}M as a positive control; COR37.5 . addition of 37.5 {mu}L of EO in 75 mL of buffered rumen fluid; COR75 - addition of 75 {mu}L of EO in 75 mL of buffered rumen fluid. A complete randomized design was utilized with six replicates for gas production (mL/g OM{sub incub}) and three replicates for all other variables. Two conditions were independently assessed: a) Coastcross (Cynodon sp.) hay (89.2% DM, 9.7% CP, 1.3% EE, 7.9% ash, 60.2% NDF, and 30.6% ADF) as substrate + inoculum of sheep on pasture; b) 80:20 concentrate:forage diet (20.0% Coastcross hay, 62.7% corn, 15.0% soybean meal, 1.0% limestone, and 1.3% mineral premix on DM basis; 91.5% DM, 15.7% CP, 3.3% EE, 4.3%  More>>
Authors:
Araujo, R C; Pires, A V; [1]  Abdalla, A.L. , E-mail: rcanonenco@hotmail.com (and others) [2] 
  1. ESALQ, Universidade de Sao Paulo, Piracicaba, SP (Brazil)
  2. CENA, Universidade de Sao Paulo, Piracicaba, SP (Brazil)
Publication Date:
Jul 01, 2009
Product Type:
Conference
Report Number:
IAEA-CN-174; IAEA-CN-174/81
Resource Relation:
Conference: FAO/IAEA international symposium on sustainable improvement of animal production and health, Vienna (Austria), 8-11 Jun 2009; Other Information: 2 refs, 2 tabs; Related Information: In: FAO/IAEA international symposium on sustainable improvement of animal production and health. Synopses, 461 pages.
Subject:
60 APPLIED LIFE SCIENCES; DIET; ESSENTIAL OILS; FERMENTATION; IN VITRO; METHANE; PRODUCTION; SHEEP; STOMACH
OSTI ID:
21301740
Research Organizations:
Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna (Austria); United Nations, New York, NY (United States); World Organization for Animal Health, Paris (France); World Health Organization, Geneva (Switzerland); European Commission, Brussels (Belgium)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
TRN: XA0900559046539
Availability:
Available from INIS in electronic form. Also available on-line: http://www-naweb.iaea.org/nafa/aph/BookOfExtendedSynopses.pdf
Submitting Site:
INIS
Size:
page(s) 170-171
Announcement Date:
Jun 10, 2010

Conference:

Citation Formats

Araujo, R C, Pires, A V, and Abdalla, A.L. , E-mail: rcanonenco@hotmail.com (and others). Effect of essential oil from Cordia verbeancea on the fermentation of a high concentrate diet by using the in vitro gas production technique. IAEA: N. p., 2009. Web.
Araujo, R C, Pires, A V, & Abdalla, A.L. , E-mail: rcanonenco@hotmail.com (and others). Effect of essential oil from Cordia verbeancea on the fermentation of a high concentrate diet by using the in vitro gas production technique. IAEA.
Araujo, R C, Pires, A V, and Abdalla, A.L. , E-mail: rcanonenco@hotmail.com (and others). 2009. "Effect of essential oil from Cordia verbeancea on the fermentation of a high concentrate diet by using the in vitro gas production technique." IAEA.
@misc{etde_21301740,
title = {Effect of essential oil from Cordia verbeancea on the fermentation of a high concentrate diet by using the in vitro gas production technique}
author = {Araujo, R C, Pires, A V, and Abdalla, A.L. , E-mail: rcanonenco@hotmail.com (and others)}
abstractNote = {Studies with plant secondary metabolites as rumen fermentation modifiers have increased as an attempt to reproduce the effects of ionophores. Cordia verbenacea D.C. is a Brazilian bush with antimicrobial properties attributed to its essential oil (EO). The objective of this experiment was to determine the effects of C. verbenacea EO on the ruminal fermentation by using an in vitro gas production system. Treatments were defined as: Control - without addition of monensin or EO; MON - addition of monensin (Sigma Aldrich Inc.) at 3 {mu}M as a positive control; COR37.5 . addition of 37.5 {mu}L of EO in 75 mL of buffered rumen fluid; COR75 - addition of 75 {mu}L of EO in 75 mL of buffered rumen fluid. A complete randomized design was utilized with six replicates for gas production (mL/g OM{sub incub}) and three replicates for all other variables. Two conditions were independently assessed: a) Coastcross (Cynodon sp.) hay (89.2% DM, 9.7% CP, 1.3% EE, 7.9% ash, 60.2% NDF, and 30.6% ADF) as substrate + inoculum of sheep on pasture; b) 80:20 concentrate:forage diet (20.0% Coastcross hay, 62.7% corn, 15.0% soybean meal, 1.0% limestone, and 1.3% mineral premix on DM basis; 91.5% DM, 15.7% CP, 3.3% EE, 4.3% ash, 20.3% NDF, and 8.8% ADF) as substrate + inoculum of sheep adapted to this diet. Two different inocula for each condition were used as source of variation. In each flask (160 mL), 0.5 g of substrate was incubated with 50 mL of incubation medium and 25 mL of rumen fluid at 39 deg C. Incubation time was 24 h for the hay and 16 h for the high-concentrate diet. Flasks without substrate (blanks) and flasks containing standard hay were also included. According to the GC-MS analysis, the major compounds of C. verbenacea EO were: transcaryophyllene (28.19%), alpha-pinene (23.58%), aloaromadendrene (6.90%), and alpha-humulene (4.54%). Considering both substrates, MON reduced gas and methane productions, increased propionate concentration, and decreased acetate:propionate ratio when compared with Control. Especially for hay, TDDOM was reduced by MON and COR75, which is at least for MON a limitation imposed by short-term in vitro trials. The most promising effect observed with EO inclusion was related to the inhibition of methanogenesis using hay as substrate. Methane produced per unit of OM{sub incub} was reduced by 30% comparing COR75 with Control. Although not statistically different, methane production expressed as mL/g OM{sub degrad} showed an intermediary value for COR75 (32.9) when contrasted with Control (38.9) and MON (25.8). The partitioning factor was reduced comparing COR75 with Control in the hay condition, which indicates lower microbial production efficiency. In general, no effects were observed with EO inclusion using the high-concentrate diet as substrate. In this condition, the doses seemed to be very low to manipulate rumen fermentation. In conclusion, the EO from C. verbenacea was able to modify in vitro ruminal fermentation. This study indicates that doses greater than 1 {mu}L/mL of buffered rumen fluid may decrease methane production as much as monensin when hay is used as substrate. (author)}
place = {IAEA}
year = {2009}
month = {Jul}
}