Abstract
Using in vivo molecular imaging with microPET/SPECT has been expected to facilitate drug discovery and development. In this study, we applied quantitative microPET to the preclinical evaluation of the effects of two neuroprotective drug candidates to the nigrostriatal dopaminergic neuronal damage. Fifteen SD rats were divided into three groups. The rats of each group were orally administrated one of neuroprotective candidate; NeuProtec (100mg/kg bid) and SureCero (10mg/kg, qd) or normal saline (0.1ml, qd) for 3 weeks. 6-OHDA was sterotactically placed to the right striatum on eighth day after starting while continuing the medication for additional 14 days. [{sup 124}I]FP-ClT PET scans were obtained using microPET R4 scanner. The behavioral test by amphetamine-induced rotation and the histological examination after thyrosine hydroxylase (TH) immunohistochemical staining were performed. Different uptake in the lesioned striatum among the groups were demonstrated on [{sup 124}I]FP-CIT PET images. The rats with NeuProtec showed higher binding in the lesion than controls. No differences were observed in SureCere groups. The FP-CIT uptake in the lesioned striatum was well correlated with the % reduction of TH(+) cells (rho =0.73, p=0.025), and also correlated with rotation test (rho =0.79, p=0.001) [{sup 124}I]FP-CIT animal PET depicted the neuroprotective effects of NeuProtec to
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Son, Ji Yeon;
Kim, Yu Kyeong;
Kim, Ji Sun;
Lee, Byung Chul;
Kim, Kyeong Min;
Choi, Tae Hyun;
Cheon, Gi Jeong;
Lee, Won Woo;
Kim, Sang Eun
[1]
- Seoul National Univ. College of Medicine, Seoul (Korea, Republic of)
Citation Formats
Son, Ji Yeon, Kim, Yu Kyeong, Kim, Ji Sun, Lee, Byung Chul, Kim, Kyeong Min, Choi, Tae Hyun, Cheon, Gi Jeong, Lee, Won Woo, and Kim, Sang Eun.
Preclinical quantitative MicroPET imaging in evaluation of neuroprotective drug candidates.
Korea, Republic of: N. p.,
2007.
Web.
Son, Ji Yeon, Kim, Yu Kyeong, Kim, Ji Sun, Lee, Byung Chul, Kim, Kyeong Min, Choi, Tae Hyun, Cheon, Gi Jeong, Lee, Won Woo, & Kim, Sang Eun.
Preclinical quantitative MicroPET imaging in evaluation of neuroprotective drug candidates.
Korea, Republic of.
Son, Ji Yeon, Kim, Yu Kyeong, Kim, Ji Sun, Lee, Byung Chul, Kim, Kyeong Min, Choi, Tae Hyun, Cheon, Gi Jeong, Lee, Won Woo, and Kim, Sang Eun.
2007.
"Preclinical quantitative MicroPET imaging in evaluation of neuroprotective drug candidates."
Korea, Republic of.
@misc{etde_21243750,
title = {Preclinical quantitative MicroPET imaging in evaluation of neuroprotective drug candidates}
author = {Son, Ji Yeon, Kim, Yu Kyeong, Kim, Ji Sun, Lee, Byung Chul, Kim, Kyeong Min, Choi, Tae Hyun, Cheon, Gi Jeong, Lee, Won Woo, and Kim, Sang Eun}
abstractNote = {Using in vivo molecular imaging with microPET/SPECT has been expected to facilitate drug discovery and development. In this study, we applied quantitative microPET to the preclinical evaluation of the effects of two neuroprotective drug candidates to the nigrostriatal dopaminergic neuronal damage. Fifteen SD rats were divided into three groups. The rats of each group were orally administrated one of neuroprotective candidate; NeuProtec (100mg/kg bid) and SureCero (10mg/kg, qd) or normal saline (0.1ml, qd) for 3 weeks. 6-OHDA was sterotactically placed to the right striatum on eighth day after starting while continuing the medication for additional 14 days. [{sup 124}I]FP-ClT PET scans were obtained using microPET R4 scanner. The behavioral test by amphetamine-induced rotation and the histological examination after thyrosine hydroxylase (TH) immunohistochemical staining were performed. Different uptake in the lesioned striatum among the groups were demonstrated on [{sup 124}I]FP-CIT PET images. The rats with NeuProtec showed higher binding in the lesion than controls. No differences were observed in SureCere groups. The FP-CIT uptake in the lesioned striatum was well correlated with the % reduction of TH(+) cells (rho =0.73, p=0.025), and also correlated with rotation test (rho =0.79, p=0.001) [{sup 124}I]FP-CIT animal PET depicted the neuroprotective effects of NeuProtec to the 6-OHDA neurotoxicity in the rat striatum. No demonstrable effect of SureCero might indicate that inadequate dosage was used in this study. MicroPET imaging with small animal could be a great tool in preclinical evaluation of drug efficacy.}
place = {Korea, Republic of}
year = {2007}
month = {Jul}
}
title = {Preclinical quantitative MicroPET imaging in evaluation of neuroprotective drug candidates}
author = {Son, Ji Yeon, Kim, Yu Kyeong, Kim, Ji Sun, Lee, Byung Chul, Kim, Kyeong Min, Choi, Tae Hyun, Cheon, Gi Jeong, Lee, Won Woo, and Kim, Sang Eun}
abstractNote = {Using in vivo molecular imaging with microPET/SPECT has been expected to facilitate drug discovery and development. In this study, we applied quantitative microPET to the preclinical evaluation of the effects of two neuroprotective drug candidates to the nigrostriatal dopaminergic neuronal damage. Fifteen SD rats were divided into three groups. The rats of each group were orally administrated one of neuroprotective candidate; NeuProtec (100mg/kg bid) and SureCero (10mg/kg, qd) or normal saline (0.1ml, qd) for 3 weeks. 6-OHDA was sterotactically placed to the right striatum on eighth day after starting while continuing the medication for additional 14 days. [{sup 124}I]FP-ClT PET scans were obtained using microPET R4 scanner. The behavioral test by amphetamine-induced rotation and the histological examination after thyrosine hydroxylase (TH) immunohistochemical staining were performed. Different uptake in the lesioned striatum among the groups were demonstrated on [{sup 124}I]FP-CIT PET images. The rats with NeuProtec showed higher binding in the lesion than controls. No differences were observed in SureCere groups. The FP-CIT uptake in the lesioned striatum was well correlated with the % reduction of TH(+) cells (rho =0.73, p=0.025), and also correlated with rotation test (rho =0.79, p=0.001) [{sup 124}I]FP-CIT animal PET depicted the neuroprotective effects of NeuProtec to the 6-OHDA neurotoxicity in the rat striatum. No demonstrable effect of SureCero might indicate that inadequate dosage was used in this study. MicroPET imaging with small animal could be a great tool in preclinical evaluation of drug efficacy.}
place = {Korea, Republic of}
year = {2007}
month = {Jul}
}