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Specific detection of oxytetracycline using DNA aptamer-immobilized interdigitated array electrode chip

Abstract

An electrochemical sensing system for oxytetracycline (OTC) detection was developed using ssDNA aptamer immobilized on gold interdigitated array (IDA) electrode chip. A highly specific ssDNA aptamer that bind to OTC with high affinity was employed to discriminate other tetracyclines (TCs), such as doxycycline (DOX) and tetracycline (TET). The immobilized thiol-modified aptamer on gold electrode chip served as a biorecognition element for the target molecules and the electrochemical signals generated from interactions between the aptamers and the target molecules was evaluated by cyclic voltammetry (CV) and square wave voltammetry (SWV). The current decrease due to the interference of bound OTC, DOX or TET was analyzed with the electron flow produced by a redox reaction between ferro- and ferricyanide. The specificity of developed EC-biosensor for OTC was highly distinguishable from the structurally similar antibiotics (DOX and TET). The dynamic range was determined to be 1-100 nM of OTC concentration in semi-logarithmic coordinates.
Authors:
Kim, Yeon Seok; Niazi, Javed H; [1]  Gu, Man Bock , E-mail: mbgu@korea.ac.kr [1] 
  1. School of Life Sciences and Biotechnology, Korea University, Anam-dong, Seongbuk-gu, Seoul 136-701 (Korea, Republic of)
Publication Date:
Feb 23, 2009
Product Type:
Journal Article
Resource Relation:
Journal Name: Analytica Chimica Acta; Journal Volume: 634; Journal Issue: 2; Other Information: DOI: 10.1016/j.aca.2008.12.025; PII: S0003-2670(08)02180-6; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; DNA; ELECTROCHEMISTRY; ELECTRODES; FERRICYANIDES; GOLD; OXYTETRACYCLINE; REDOX REACTIONS; THIOLS; VOLTAMETRY
OSTI ID:
21207444
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0003-2670; ACACAM; TRN: NL09R0077078193
Availability:
Available from http://dx.doi.org/10.1016/j.aca.2008.12.025;INIS
Submitting Site:
NLN
Size:
page(s) 250-254
Announcement Date:
Sep 02, 2009

Citation Formats

Kim, Yeon Seok, Niazi, Javed H, and Gu, Man Bock , E-mail: mbgu@korea.ac.kr. Specific detection of oxytetracycline using DNA aptamer-immobilized interdigitated array electrode chip. Netherlands: N. p., 2009. Web. doi:10.1016/j.aca.2008.12.025.
Kim, Yeon Seok, Niazi, Javed H, & Gu, Man Bock , E-mail: mbgu@korea.ac.kr. Specific detection of oxytetracycline using DNA aptamer-immobilized interdigitated array electrode chip. Netherlands. doi:10.1016/j.aca.2008.12.025.
Kim, Yeon Seok, Niazi, Javed H, and Gu, Man Bock , E-mail: mbgu@korea.ac.kr. 2009. "Specific detection of oxytetracycline using DNA aptamer-immobilized interdigitated array electrode chip." Netherlands. doi:10.1016/j.aca.2008.12.025. https://www.osti.gov/servlets/purl/10.1016/j.aca.2008.12.025.
@misc{etde_21207444,
title = {Specific detection of oxytetracycline using DNA aptamer-immobilized interdigitated array electrode chip}
author = {Kim, Yeon Seok, Niazi, Javed H, and Gu, Man Bock , E-mail: mbgu@korea.ac.kr}
abstractNote = {An electrochemical sensing system for oxytetracycline (OTC) detection was developed using ssDNA aptamer immobilized on gold interdigitated array (IDA) electrode chip. A highly specific ssDNA aptamer that bind to OTC with high affinity was employed to discriminate other tetracyclines (TCs), such as doxycycline (DOX) and tetracycline (TET). The immobilized thiol-modified aptamer on gold electrode chip served as a biorecognition element for the target molecules and the electrochemical signals generated from interactions between the aptamers and the target molecules was evaluated by cyclic voltammetry (CV) and square wave voltammetry (SWV). The current decrease due to the interference of bound OTC, DOX or TET was analyzed with the electron flow produced by a redox reaction between ferro- and ferricyanide. The specificity of developed EC-biosensor for OTC was highly distinguishable from the structurally similar antibiotics (DOX and TET). The dynamic range was determined to be 1-100 nM of OTC concentration in semi-logarithmic coordinates.}
doi = {10.1016/j.aca.2008.12.025}
journal = {Analytica Chimica Acta}
issue = {2}
volume = {634}
place = {Netherlands}
year = {2009}
month = {Feb}
}