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A reversed-phase high performance liquid chromatography coupled with resonance Rayleigh scattering detection for the determination of four tetracycline antibiotics

Journal Article:

Abstract

A new reversed-phase high performance liquid chromatography with resonance Rayleigh scattering detection (HPLC-RRS) was developed for simultaneous separation and determination of four tetracycline antibiotics (TCs). A good chromatographic separation among the compounds was achieved using a Synergi Fusion-RP column (150 mm x 4.6 mm; 4 {mu}m) and a mobile phase consisting of methanol-acetonitrile-oxalic acid (5 mM) at the flow rate of 0.8 mL min{sup -1}. Column temperature was 30 deg. C. The RRS signal was detected at {lambda}{sub ex} = {lambda}{sub em} = 370 nm. The recoveries of sample added standard ranged from 95.3% to 103.5%, and the relative standard deviation was below 2.79%. A detection limit of 2.12-5.12 {mu}g mL{sup -1} was reached and a linear range was found between peak height and concentration in the range of 10.36-518.0 {mu}g mL{sup -1} for oxytetracycline (OTC), 12.11-605.5 {mu}g mL{sup -1} for tetracycline (TC), 11.79-589.5 {mu}g mL{sup -1} for chlortetracycline (CTC) and 10.32-516.0 {mu}g mL{sup -1} for doxycycline (DC). The linear regression coefficients were all above 0.999. The method has been applied successfully to the determination of OTC, TC, CTC, DC in pharmaceutical formulations and in honey. The method was simple, rapid and showed a better linear relation and high repeatability.
Authors:
Lifeng, Wang; [1]  Peng Jingdong , E-mail: hxpengjd@swu.edu.cn; [1]  Limin, Liu [1] 
  1. School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715 (China)
Publication Date:
Dec 07, 2008
Product Type:
Journal Article
Resource Relation:
Journal Name: Analytica Chimica Acta; Journal Volume: 630; Journal Issue: 1; Other Information: DOI: 10.1016/j.aca.2008.09.066; PII: S0003-2670(08)01743-1; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; OXYTETRACYCLINE; RAYLEIGH SCATTERING; SENSITIVITY; TEMPERATURE RANGE 0273-0400 K
OSTI ID:
21207385
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0003-2670; ACACAM; TRN: NL09R0015078134
Availability:
Available from http://dx.doi.org/10.1016/j.aca.2008.09.066;INIS
Submitting Site:
NLN
Size:
page(s) 101-106
Announcement Date:
Sep 02, 2009

Journal Article:

Citation Formats

Lifeng, Wang, Peng Jingdong , E-mail: hxpengjd@swu.edu.cn, and Limin, Liu. A reversed-phase high performance liquid chromatography coupled with resonance Rayleigh scattering detection for the determination of four tetracycline antibiotics. Netherlands: N. p., 2008. Web.
Lifeng, Wang, Peng Jingdong , E-mail: hxpengjd@swu.edu.cn, & Limin, Liu. A reversed-phase high performance liquid chromatography coupled with resonance Rayleigh scattering detection for the determination of four tetracycline antibiotics. Netherlands.
Lifeng, Wang, Peng Jingdong , E-mail: hxpengjd@swu.edu.cn, and Limin, Liu. 2008. "A reversed-phase high performance liquid chromatography coupled with resonance Rayleigh scattering detection for the determination of four tetracycline antibiotics." Netherlands.
@misc{etde_21207385,
title = {A reversed-phase high performance liquid chromatography coupled with resonance Rayleigh scattering detection for the determination of four tetracycline antibiotics}
author = {Lifeng, Wang, Peng Jingdong , E-mail: hxpengjd@swu.edu.cn, and Limin, Liu}
abstractNote = {A new reversed-phase high performance liquid chromatography with resonance Rayleigh scattering detection (HPLC-RRS) was developed for simultaneous separation and determination of four tetracycline antibiotics (TCs). A good chromatographic separation among the compounds was achieved using a Synergi Fusion-RP column (150 mm x 4.6 mm; 4 {mu}m) and a mobile phase consisting of methanol-acetonitrile-oxalic acid (5 mM) at the flow rate of 0.8 mL min{sup -1}. Column temperature was 30 deg. C. The RRS signal was detected at {lambda}{sub ex} = {lambda}{sub em} = 370 nm. The recoveries of sample added standard ranged from 95.3% to 103.5%, and the relative standard deviation was below 2.79%. A detection limit of 2.12-5.12 {mu}g mL{sup -1} was reached and a linear range was found between peak height and concentration in the range of 10.36-518.0 {mu}g mL{sup -1} for oxytetracycline (OTC), 12.11-605.5 {mu}g mL{sup -1} for tetracycline (TC), 11.79-589.5 {mu}g mL{sup -1} for chlortetracycline (CTC) and 10.32-516.0 {mu}g mL{sup -1} for doxycycline (DC). The linear regression coefficients were all above 0.999. The method has been applied successfully to the determination of OTC, TC, CTC, DC in pharmaceutical formulations and in honey. The method was simple, rapid and showed a better linear relation and high repeatability.}
journal = {Analytica Chimica Acta}
issue = {1}
volume = {630}
place = {Netherlands}
year = {2008}
month = {Dec}
}