You need JavaScript to view this

Preparation and characterization of gold nanocrystals and nanomultilayer mirrors for X-ray diffraction experiments; Praeparation und Charakterisierung von Goldnanokristallen und Nanomultilayerspiegeln fuer Roentgenbeugungsexperimente

Abstract

In order to make possible studies on the dynamics of protein molecules in their natural environment Sasaki has developed in the last years a new X-ray diffraction procedure. In this procedure, which is called dynamical X-ray tracking (DXT), the diffraction occurs not directly on the protein molecule, but on a nanomirror rigidly bound to the protein molecule. Measured is hereby the time variation od the alignment of the nanocrystal, which is determined by means of the position of the Laue-diffraction points. By means of these position variations statements on structure variations of the studied protein can be derived with a high spatial accuracy in the time domain. The scientific aim of this thesis is the construction of a DXT measuring place as well as the preparation of the requireds nanocrystalline X-ray diffracting protein labels including their characterization. First a short survey about the foundations of the X radiation and their interactions with matter, especially under regardment of X-ray diffraction on crystals, is given. The measuring methods for the determination of the crystal alignment as well as the vertical and lateral crystal size are presented. In the following chapter a comprehensive survey about the different devices and analysis methods used for  More>>
Authors:
Publication Date:
Mar 15, 2009
Product Type:
Thesis/Dissertation
Report Number:
INIS-DE-0704
Resource Relation:
Other Information: TH: Diss.
Subject:
75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; PROTEINS; MOLECULES; ALIGNMENT; FABRICATION; GOLD; MOLECULAR BEAM EPITAXY; PHOTON BEAMS; BEAM PROFILES; FOCUSING; X-RAY SPECTRA; DAMAGE; CYSTEINE; COLLOIDS; SHAPE; SUBSTRATES; MONOCRYSTALS; MIRRORS; TRANSMISSION ELECTRON MICROSCOPY; X-RAY DIFFRACTION; NANOSTRUCTURES; PHYSICAL RADIATION EFFECTS; X RADIATION; MOLYBDENUM; SILICON; X-RAY EQUIPMENT; LAUE METHOD; SPATIAL RESOLUTION; ATOMIC FORCE MICROSCOPY; SODIUM CHLORIDES; SURFACES
OSTI ID:
21196653
Research Organizations:
Bielefeld Univ. (Germany). Fakultaet fuer Physik
Country of Origin:
Germany
Language:
German
Other Identifying Numbers:
TRN: DE09F7599
Availability:
Commercial reproduction prohibited; INIS; OSTI as DE21196653
Submitting Site:
DEN
Size:
148 pages
Announcement Date:
Jul 22, 2009

Citation Formats

Slieh, Jawad. Preparation and characterization of gold nanocrystals and nanomultilayer mirrors for X-ray diffraction experiments; Praeparation und Charakterisierung von Goldnanokristallen und Nanomultilayerspiegeln fuer Roentgenbeugungsexperimente. Germany: N. p., 2009. Web.
Slieh, Jawad. Preparation and characterization of gold nanocrystals and nanomultilayer mirrors for X-ray diffraction experiments; Praeparation und Charakterisierung von Goldnanokristallen und Nanomultilayerspiegeln fuer Roentgenbeugungsexperimente. Germany.
Slieh, Jawad. 2009. "Preparation and characterization of gold nanocrystals and nanomultilayer mirrors for X-ray diffraction experiments; Praeparation und Charakterisierung von Goldnanokristallen und Nanomultilayerspiegeln fuer Roentgenbeugungsexperimente." Germany.
@misc{etde_21196653,
title = {Preparation and characterization of gold nanocrystals and nanomultilayer mirrors for X-ray diffraction experiments; Praeparation und Charakterisierung von Goldnanokristallen und Nanomultilayerspiegeln fuer Roentgenbeugungsexperimente}
author = {Slieh, Jawad}
abstractNote = {In order to make possible studies on the dynamics of protein molecules in their natural environment Sasaki has developed in the last years a new X-ray diffraction procedure. In this procedure, which is called dynamical X-ray tracking (DXT), the diffraction occurs not directly on the protein molecule, but on a nanomirror rigidly bound to the protein molecule. Measured is hereby the time variation od the alignment of the nanocrystal, which is determined by means of the position of the Laue-diffraction points. By means of these position variations statements on structure variations of the studied protein can be derived with a high spatial accuracy in the time domain. The scientific aim of this thesis is the construction of a DXT measuring place as well as the preparation of the requireds nanocrystalline X-ray diffracting protein labels including their characterization. First a short survey about the foundations of the X radiation and their interactions with matter, especially under regardment of X-ray diffraction on crystals, is given. The measuring methods for the determination of the crystal alignment as well as the vertical and lateral crystal size are presented. In the following chapter a comprehensive survey about the different devices and analysis methods used for the fabrication and characterization of gold crystals is presented. Additionally with precise technical statements the self-constructed MBE apparature is described. This apparature has the purpose to fabricate gold nanocrystals by means of the molecular-beam-epitaxy (MBE) procedure. In the fourth chapter the construction of the DXT laboratory are presented and its beam profile in the focus, its divergence, and its beam spectrum determined. Based on this in the fifth chapter the study of the radiation damage of 2 cysteine-peroxyredoxine (2CP) proteins and the detection of this radiation damage without Au colloids and with Au colloids are presented. The main content of the sixth chapter is the precise description of the preparation of gold nano- and microcrystals. In the chapters seven and eight the results of the characterization of the gold nano- as well as microcrystals are considere. Especially hereby the influence of different preparation parameters on the crystal size as well as size distribution, the crystal shape, the crystal alignment relatively to the substrate as well as the contribution of the monocrystals is studied. In chapter nine the structure studies performed by means of TEM measurements on free gold crystals as well as Mo/Si nanomultilayer mirrors are presented. [German] Um Untersuchungen zur Dynamik von Proteinmolekuelen in ihrer natuerlichen Umgebung zu ermoeglichen, hat Sasaki in den letzten Jahren ein neues Roentgenbeugungsverfahren entwickelt. Bei diesem Verfahren, das Dynamical X-Ray-Tracking (DXT) genannt wird, findet die Beugung nicht direkt am Proteinmolekuel, sondern an einem starr an das Proteinmolekuel gebundenen Nanospiegel statt. Gemessen wird hierbei die zeitliche Veraenderung der Ausrichtung des Nanokristalls, die anhand der Lage der Laue-Beugungspunkte bestimmt wird. Anhand dieser Lageveraenderungen lassen sich dann wieder Aussagen ueber Strukturaenderungen des untersuchten Proteins mit einer hohen Ortsgenauigkeit in der Zeitdomaene herleiten. Die wissenschaftliche Zielsetzung dieser Arbeit ist der Aufbau eines DXT-Messplatzes sowie die Praeparation der benoetigten nanokristallinen roentgenbeugenden Proteinlabels einschliesslich deren Charakterisierung. Zunaechst wird ein kurzer Ueberblick ueber die Grundlagen der Roentgenstrahlung und deren Wechselwirkung mit Materie, insbesondere unter Beruecksichtigung von Roentgenbeugung an Kristallen, gegeben. Es werden die Messmethoden zur Bestimmung der Kristallausrichtung sowie der vertikalen und lateralen Kristallgroesse dargestellt. Im darauf folgenden Kapitel wird ein umfassender Ueberblick ueber die verschiedenen zur Herstellung und Charakterisierung von Goldkristallen verwendeten Geraete und Analysemethoden vorgestellt. Zusaetzlich wird hier mit genauen technischen Angaben die selbstgebaute MBE-Apparatur beschrieben. Diese Apparatur hat den Zweck, Nanogoldkristalle mit Hilfe des Molekularstrahlepitaxie-(MBE-)Verfahrens herzustellen. Im vierten Kapitel werden der Aufbau des DXT-Laborsystems dargestellt und sein Strahlprofil im Fokus, seine Divergenz und sein Strahlspektrum bestimmt. Hierauf aufbauend wird im fuenften Kapitel die Untersuchung der Strahlenschaedigung von 2 Cystein-Peroxyredoxin (2CP) Proteinen und der Nachweis dieser Strahlenschaedigung ohne Au-Kolloiden und mit Au-Kolloiden dargestellt. Der Hauptinhalt des sechsten Kapitels ist die genaue Beschreibung der Praeparation von Nano- und Mikro-Goldkristallen. In den Kapiteln sieben und acht wird auf die Ergebnisse der Charakterisierung der nano- sowie mikrokristallinen Goldkristalle eingegangen. Insbesondere wird dabei der Einfluss verschiedener Praeparationsparameter auf die Kristallgroesse sowie Groessenverteilung, die Kristallform, die Kristallausrichtung bezueglich des Substrates sowie der Anteil der Einkristalle untersucht. In Kapitel neun werden die mittels TEM-Messungen durchgefuehrten Strukturuntersuchungen an freien Goldkristallen sowie nano-Mo/Si-Multilayerspiegeln vorgestellt. (orig.)}
place = {Germany}
year = {2009}
month = {Mar}
}