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Protective effects of edaravone on the radiation response of oligodendrocyte in rats following whole brain irradiation

Abstract

Objective: To investigate the changes of the oligodendrocyte lineage cells in the cortex following whole brain irradiation and the effects of the neotype free radical scavenger, edaravone on radiation response of oligodendrocyte in rats. Methods: 120 male Sprague Dawley rats were randomly divided into sham- irradiation group, irradiation group and edaravone group. The model of whole-brain irradiation was established with exposure of the whole brain of the rats to 4 MeV X-rays with a single-dose of 10 Gy. The rats were injected intraperitoneally with edaravone at 0.3, 1.0 and 3.0 mg/kg. Tissue microarray of irradiation-induced brain injury in rats was constructed. The expression of A2BS, oligodendrocyte market 4(O4) and 2', 3'-cyclic nucleotide 3'- phosphodiesterase (CNPase) in the cortex was examined by tissue microarray technology and immunohistochemistry. The positive cells were counted. Results: Compared with the sham-irradiation group, the number of A2BS-positive cells increased and the number of O4, CNPase-positive cells decreased significantly at certain time in the irradiation group(P<0.05). Compared with irradiation group, A2BS-positive cells decreased significantly after edaravone treatment, while O4-positive cells and CNPase-positive cells increased significantly (P<0.05, or P<0.01). Conclusions: The number of oligodendrocyte precursor cells in the cortex of rats increased reactively following whole brain irradiation and  More>>
Authors:
Yingzhu, Chen; Ye, Tian; Shiyao, Bao; Huan, Bao; Zhilin, Zhan [1] 
  1. The Second Affiliated Hospital of Suzhou Univ., Suzhou (China)
Publication Date:
Aug 15, 2007
Product Type:
Journal Article
Resource Relation:
Journal Name: Chinese Journal of Radiological Medicine and Protection; Journal Volume: 27; Journal Issue: 4; Other Information: 1 fig., 1 tab., 14 refs
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; ANIMAL CELLS; BRAIN; CEREBRAL CORTEX; DOSE-RESPONSE RELATIONSHIPS; INTRAPERITONEAL INJECTION; IRRADIATION; MICROARRAY TECHNOLOGY; RADIATION INJURIES; RADIOPROTECTIVE SUBSTANCES; RATS; X RADIATION
OSTI ID:
21188222
Country of Origin:
China
Language:
Chinese
Other Identifying Numbers:
Journal ID: ISSN 0254-5098; ZFYZDY; TRN: CN0901609060322
Submitting Site:
INIS
Size:
page(s) 328-331
Announcement Date:
Aug 17, 2009

Citation Formats

Yingzhu, Chen, Ye, Tian, Shiyao, Bao, Huan, Bao, and Zhilin, Zhan. Protective effects of edaravone on the radiation response of oligodendrocyte in rats following whole brain irradiation. China: N. p., 2007. Web.
Yingzhu, Chen, Ye, Tian, Shiyao, Bao, Huan, Bao, &amp; Zhilin, Zhan. Protective effects of edaravone on the radiation response of oligodendrocyte in rats following whole brain irradiation. China.
Yingzhu, Chen, Ye, Tian, Shiyao, Bao, Huan, Bao, and Zhilin, Zhan. 2007. "Protective effects of edaravone on the radiation response of oligodendrocyte in rats following whole brain irradiation." China.
@misc{etde_21188222,
title = {Protective effects of edaravone on the radiation response of oligodendrocyte in rats following whole brain irradiation}
author = {Yingzhu, Chen, Ye, Tian, Shiyao, Bao, Huan, Bao, and Zhilin, Zhan}
abstractNote = {Objective: To investigate the changes of the oligodendrocyte lineage cells in the cortex following whole brain irradiation and the effects of the neotype free radical scavenger, edaravone on radiation response of oligodendrocyte in rats. Methods: 120 male Sprague Dawley rats were randomly divided into sham- irradiation group, irradiation group and edaravone group. The model of whole-brain irradiation was established with exposure of the whole brain of the rats to 4 MeV X-rays with a single-dose of 10 Gy. The rats were injected intraperitoneally with edaravone at 0.3, 1.0 and 3.0 mg/kg. Tissue microarray of irradiation-induced brain injury in rats was constructed. The expression of A2BS, oligodendrocyte market 4(O4) and 2', 3'-cyclic nucleotide 3'- phosphodiesterase (CNPase) in the cortex was examined by tissue microarray technology and immunohistochemistry. The positive cells were counted. Results: Compared with the sham-irradiation group, the number of A2BS-positive cells increased and the number of O4, CNPase-positive cells decreased significantly at certain time in the irradiation group(P<0.05). Compared with irradiation group, A2BS-positive cells decreased significantly after edaravone treatment, while O4-positive cells and CNPase-positive cells increased significantly (P<0.05, or P<0.01). Conclusions: The number of oligodendrocyte precursor cells in the cortex of rats increased reactively following whole brain irradiation and changed with time. Edaravone played a protective role in oligodendrocyte ischemic reaction in a dose-dependent manner. (authors)}
journal = []
issue = {4}
volume = {27}
place = {China}
year = {2007}
month = {Aug}
}