Abstract
Objective: To investigate the transfection and expression of p53 genes mediated by liposome and its feasibility in treatment of liver cancer by transcatheter arterial injection on rabbit VX2 hepatocarcinoma model. Methods: pCMV-myc-p53 plasmids, LipofectAMINE and p53-LipofectAMINE complex were infused into tumor's feeding artery of rabbit VX2 hepatocarcinoma model, respectively, and then protein of cancer tissue was extracted, followed by measuring gene transfection and expression by western blot and immunohistochemistry, p53-LipofectAMlNE complex in different doses were infused into tumor's feeding artery of rabbit VX2 hepatocarcinoma model with the gene transfection and expression detected by the same way. Results: Liposome-mediated p53 gene injected through catheter could be successfully transfected and expressed in the cancer tissue of rabbit VX2 hepatocarcinoma model, with transfection efficiency higher than the gene delivery alone. The efficiency and the gene dose has dose-effect relationship. Conclusions: Treatment of liver cancer by transcatheter arterial injection of p53 genes mediated by liposome is a feasible and effective method, with wide prospect of application. (authors)
Guangyu, Zhu;
Qin, Lu;
Gaojun, Teng;
Jinhe, Guo;
Hui, Yu;
Gang, Deng;
Shicheng, He;
Wen, Fang;
Guozhao, Li;
Xiaoying, Wei
[1]
- Zhongda Hospital, Southeast Univ., Nanjing (China)
Citation Formats
Guangyu, Zhu, Qin, Lu, Gaojun, Teng, Jinhe, Guo, Hui, Yu, Gang, Deng, Shicheng, He, Wen, Fang, Guozhao, Li, and Xiaoying, Wei.
Experimental research on treating hepatic carcinoma by arterial injection of liposome mediated p53 genes.
China: N. p.,
2007.
Web.
Guangyu, Zhu, Qin, Lu, Gaojun, Teng, Jinhe, Guo, Hui, Yu, Gang, Deng, Shicheng, He, Wen, Fang, Guozhao, Li, & Xiaoying, Wei.
Experimental research on treating hepatic carcinoma by arterial injection of liposome mediated p53 genes.
China.
Guangyu, Zhu, Qin, Lu, Gaojun, Teng, Jinhe, Guo, Hui, Yu, Gang, Deng, Shicheng, He, Wen, Fang, Guozhao, Li, and Xiaoying, Wei.
2007.
"Experimental research on treating hepatic carcinoma by arterial injection of liposome mediated p53 genes."
China.
@misc{etde_21164661,
title = {Experimental research on treating hepatic carcinoma by arterial injection of liposome mediated p53 genes}
author = {Guangyu, Zhu, Qin, Lu, Gaojun, Teng, Jinhe, Guo, Hui, Yu, Gang, Deng, Shicheng, He, Wen, Fang, Guozhao, Li, and Xiaoying, Wei}
abstractNote = {Objective: To investigate the transfection and expression of p53 genes mediated by liposome and its feasibility in treatment of liver cancer by transcatheter arterial injection on rabbit VX2 hepatocarcinoma model. Methods: pCMV-myc-p53 plasmids, LipofectAMINE and p53-LipofectAMINE complex were infused into tumor's feeding artery of rabbit VX2 hepatocarcinoma model, respectively, and then protein of cancer tissue was extracted, followed by measuring gene transfection and expression by western blot and immunohistochemistry, p53-LipofectAMlNE complex in different doses were infused into tumor's feeding artery of rabbit VX2 hepatocarcinoma model with the gene transfection and expression detected by the same way. Results: Liposome-mediated p53 gene injected through catheter could be successfully transfected and expressed in the cancer tissue of rabbit VX2 hepatocarcinoma model, with transfection efficiency higher than the gene delivery alone. The efficiency and the gene dose has dose-effect relationship. Conclusions: Treatment of liver cancer by transcatheter arterial injection of p53 genes mediated by liposome is a feasible and effective method, with wide prospect of application. (authors)}
journal = []
issue = {2}
volume = {16}
place = {China}
year = {2007}
month = {Feb}
}
title = {Experimental research on treating hepatic carcinoma by arterial injection of liposome mediated p53 genes}
author = {Guangyu, Zhu, Qin, Lu, Gaojun, Teng, Jinhe, Guo, Hui, Yu, Gang, Deng, Shicheng, He, Wen, Fang, Guozhao, Li, and Xiaoying, Wei}
abstractNote = {Objective: To investigate the transfection and expression of p53 genes mediated by liposome and its feasibility in treatment of liver cancer by transcatheter arterial injection on rabbit VX2 hepatocarcinoma model. Methods: pCMV-myc-p53 plasmids, LipofectAMINE and p53-LipofectAMINE complex were infused into tumor's feeding artery of rabbit VX2 hepatocarcinoma model, respectively, and then protein of cancer tissue was extracted, followed by measuring gene transfection and expression by western blot and immunohistochemistry, p53-LipofectAMlNE complex in different doses were infused into tumor's feeding artery of rabbit VX2 hepatocarcinoma model with the gene transfection and expression detected by the same way. Results: Liposome-mediated p53 gene injected through catheter could be successfully transfected and expressed in the cancer tissue of rabbit VX2 hepatocarcinoma model, with transfection efficiency higher than the gene delivery alone. The efficiency and the gene dose has dose-effect relationship. Conclusions: Treatment of liver cancer by transcatheter arterial injection of p53 genes mediated by liposome is a feasible and effective method, with wide prospect of application. (authors)}
journal = []
issue = {2}
volume = {16}
place = {China}
year = {2007}
month = {Feb}
}