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Experimental research on treating hepatic carcinoma by arterial injection of liposome mediated p53 genes

Abstract

Objective: To investigate the transfection and expression of p53 genes mediated by liposome and its feasibility in treatment of liver cancer by transcatheter arterial injection on rabbit VX2 hepatocarcinoma model. Methods: pCMV-myc-p53 plasmids, LipofectAMINE and p53-LipofectAMINE complex were infused into tumor's feeding artery of rabbit VX2 hepatocarcinoma model, respectively, and then protein of cancer tissue was extracted, followed by measuring gene transfection and expression by western blot and immunohistochemistry, p53-LipofectAMlNE complex in different doses were infused into tumor's feeding artery of rabbit VX2 hepatocarcinoma model with the gene transfection and expression detected by the same way. Results: Liposome-mediated p53 gene injected through catheter could be successfully transfected and expressed in the cancer tissue of rabbit VX2 hepatocarcinoma model, with transfection efficiency higher than the gene delivery alone. The efficiency and the gene dose has dose-effect relationship. Conclusions: Treatment of liver cancer by transcatheter arterial injection of p53 genes mediated by liposome is a feasible and effective method, with wide prospect of application. (authors)
Authors:
Guangyu, Zhu; Qin, Lu; Gaojun, Teng; Jinhe, Guo; Hui, Yu; Gang, Deng; Shicheng, He; Wen, Fang; Guozhao, Li; Xiaoying, Wei [1] 
  1. Zhongda Hospital, Southeast Univ., Nanjing (China)
Publication Date:
Feb 15, 2007
Product Type:
Journal Article
Resource Relation:
Journal Name: Journal of Interventional Radiology; Journal Volume: 16; Journal Issue: 2; Other Information: 10 figs., 16 refs
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ARTERIES; CARCINOMAS; COMPLEXES; DELIVERY; DOSES; EFFICIENCY; FEEDING; GENES; INJECTION; LIVER; PLASMIDS; PROTEINS; RABBITS; USES
OSTI ID:
21164661
Country of Origin:
China
Language:
Chinese
Other Identifying Numbers:
Journal ID: ISSN 1008-794X; TRN: CN0901258041205
Submitting Site:
INIS
Size:
page(s) 109-114
Announcement Date:
Jun 04, 2009

Citation Formats

Guangyu, Zhu, Qin, Lu, Gaojun, Teng, Jinhe, Guo, Hui, Yu, Gang, Deng, Shicheng, He, Wen, Fang, Guozhao, Li, and Xiaoying, Wei. Experimental research on treating hepatic carcinoma by arterial injection of liposome mediated p53 genes. China: N. p., 2007. Web.
Guangyu, Zhu, Qin, Lu, Gaojun, Teng, Jinhe, Guo, Hui, Yu, Gang, Deng, Shicheng, He, Wen, Fang, Guozhao, Li, & Xiaoying, Wei. Experimental research on treating hepatic carcinoma by arterial injection of liposome mediated p53 genes. China.
Guangyu, Zhu, Qin, Lu, Gaojun, Teng, Jinhe, Guo, Hui, Yu, Gang, Deng, Shicheng, He, Wen, Fang, Guozhao, Li, and Xiaoying, Wei. 2007. "Experimental research on treating hepatic carcinoma by arterial injection of liposome mediated p53 genes." China.
@misc{etde_21164661,
title = {Experimental research on treating hepatic carcinoma by arterial injection of liposome mediated p53 genes}
author = {Guangyu, Zhu, Qin, Lu, Gaojun, Teng, Jinhe, Guo, Hui, Yu, Gang, Deng, Shicheng, He, Wen, Fang, Guozhao, Li, and Xiaoying, Wei}
abstractNote = {Objective: To investigate the transfection and expression of p53 genes mediated by liposome and its feasibility in treatment of liver cancer by transcatheter arterial injection on rabbit VX2 hepatocarcinoma model. Methods: pCMV-myc-p53 plasmids, LipofectAMINE and p53-LipofectAMINE complex were infused into tumor's feeding artery of rabbit VX2 hepatocarcinoma model, respectively, and then protein of cancer tissue was extracted, followed by measuring gene transfection and expression by western blot and immunohistochemistry, p53-LipofectAMlNE complex in different doses were infused into tumor's feeding artery of rabbit VX2 hepatocarcinoma model with the gene transfection and expression detected by the same way. Results: Liposome-mediated p53 gene injected through catheter could be successfully transfected and expressed in the cancer tissue of rabbit VX2 hepatocarcinoma model, with transfection efficiency higher than the gene delivery alone. The efficiency and the gene dose has dose-effect relationship. Conclusions: Treatment of liver cancer by transcatheter arterial injection of p53 genes mediated by liposome is a feasible and effective method, with wide prospect of application. (authors)}
journal = []
issue = {2}
volume = {16}
place = {China}
year = {2007}
month = {Feb}
}