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Molecular genetic studies on some irradiated medicinal plants

Thesis/Dissertation:

Abstract

This thesis aimed to study the molecular characterization , the phylogenetic relationships among the four mentha and the three ocimum species and to get some species-specific markers. twenty-one RAPD and 10 ISSR primers were used which showed high polymorphism among the species and detected 150 molecular markers for these genotypes (100 using RAPD and 50 by ISSR-analyses). detection of the phylogenetic relationships based on the three studied systems (RAPD,ISSR and their combined analyses ) indicated that these techniques succeeded in separating the seven species into two main clusters of the two mentha and ocimum genera. SDS-protein patterns characterized the seven genotypes based on presence/ absence and staining intensities of 14 polypeptide bands into two main groups.the effect of four doses of gamma irradiation on eight active components of volatile oils and SDS-protein pattern of stems of mentha viridis indicated that low levels of gamma irradiation could improve the value of some active components of medicinal plants such as menthol in mentha viridis.
Publication Date:
Jul 01, 2007
Product Type:
Thesis/Dissertation
Resource Relation:
Other Information: TH: Thesis(MS.c); 45 tabs.,37 figs.,101 refs
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; BIOLOGICAL MARKERS; DNA; EXPERIMENTAL DATA; GAMMA RADIATION; GENETICS; MEDICINAL PLANTS; MOLECULAR BIOLOGY; POLYPEPTIDES; PROTEINS; VOLATILE MATTER
OSTI ID:
21141738
Research Organizations:
Ain Shams University, Genetics Department (Egypt)
Country of Origin:
Egypt
Language:
English
Other Identifying Numbers:
TRN: EG0900008021649
Availability:
Available from Liaison Officer for Egypt. Free of charge
Submitting Site:
INIS
Size:
148 pages
Announcement Date:
Apr 09, 2009

Thesis/Dissertation:

Citation Formats

Alam el-din, H.F.M. Molecular genetic studies on some irradiated medicinal plants. Egypt: N. p., 2007. Web.
Alam el-din, H.F.M. Molecular genetic studies on some irradiated medicinal plants. Egypt.
Alam el-din, H.F.M. 2007. "Molecular genetic studies on some irradiated medicinal plants." Egypt.
@misc{etde_21141738,
title = {Molecular genetic studies on some irradiated medicinal plants}
author = {Alam el-din, H.F.M}
abstractNote = {This thesis aimed to study the molecular characterization , the phylogenetic relationships among the four mentha and the three ocimum species and to get some species-specific markers. twenty-one RAPD and 10 ISSR primers were used which showed high polymorphism among the species and detected 150 molecular markers for these genotypes (100 using RAPD and 50 by ISSR-analyses). detection of the phylogenetic relationships based on the three studied systems (RAPD,ISSR and their combined analyses ) indicated that these techniques succeeded in separating the seven species into two main clusters of the two mentha and ocimum genera. SDS-protein patterns characterized the seven genotypes based on presence/ absence and staining intensities of 14 polypeptide bands into two main groups.the effect of four doses of gamma irradiation on eight active components of volatile oils and SDS-protein pattern of stems of mentha viridis indicated that low levels of gamma irradiation could improve the value of some active components of medicinal plants such as menthol in mentha viridis.}
place = {Egypt}
year = {2007}
month = {Jul}
}