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Genetic fingerprinting of mutant rose cultivars

Abstract

Six rose mutants evolved at the Indian Agricultural Research Institute, New Delhi from four parent cultivars were characterized based on RAPD markers. Contrary to the earlier findings our effort has conclusively proven that the RAPD markers are indeed robust tools to discern the mutants from their parents. Among 40 primers screened, 7 primers produced inconsistent banding pattern. The number of polymorphic bands varied between 4 (OPA 14) and 10 (OPA1) with an average of 6.5 bands per primer. The percentage polymorphism ranged from 62.5 (OPM 9) to 100 percent (OPA 1). Most of the primers produced monomorphic bands between parent and mutant rose cultivars. When primer OPA 2 was used a specific band of 2.5 kb was noticed in mutant cv. Pusa Urmil and cv. Pusa Abhishek but was absent in parent cv. Jantar Mantar. A polymorphic band of 750 bp was noticed in the parent Kiss of Fire and helped in differentiating the parent from its mutant when amplified with OPK 3. Primer OPS 16 produced discriminatory band of 800 bp in mutant cv. Pink Sport of Montezuma while it was absent in its parent cv. Montezuma. Another specific band of 650 bp was present in parent cv. Montezuma  More>>
Authors:
Kumar, S; Prasad, K V; Singh, K P; Singh, A.P. , E-mail: kvprasad66@gmail.com [1] 
  1. Division of Floriculture and Landscaping, Indian Agricultural Research Institute, Pusa, New Delhi (India)
Publication Date:
Jul 01, 2008
Product Type:
Conference
Report Number:
IAEA-CN-167; IAEA-CN-167-081P
Resource Relation:
Conference: International symposium on induced mutations in plants (ISIM), Vienna (Austria), 12-15 Aug 2008; Related Information: In: International symposium on induced mutations in plants (ISIM). Book of abstracts, 207 pages.
Subject:
60 APPLIED LIFE SCIENCES; ORNAMENTAL PLANTS; PLANT BREEDING; PLANT GROWTH; PRODUCTIVITY; RADIATION INDUCED MUTANTS
OSTI ID:
21141098
Research Organizations:
Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Plant Breeding Section, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
TRN: XA0801106020734
Availability:
Available from INIS in electronic form
Submitting Site:
INIS
Size:
page(s) 190
Announcement Date:
Mar 20, 2009

Citation Formats

Kumar, S, Prasad, K V, Singh, K P, and Singh, A.P. , E-mail: kvprasad66@gmail.com. Genetic fingerprinting of mutant rose cultivars. IAEA: N. p., 2008. Web.
Kumar, S, Prasad, K V, Singh, K P, & Singh, A.P. , E-mail: kvprasad66@gmail.com. Genetic fingerprinting of mutant rose cultivars. IAEA.
Kumar, S, Prasad, K V, Singh, K P, and Singh, A.P. , E-mail: kvprasad66@gmail.com. 2008. "Genetic fingerprinting of mutant rose cultivars." IAEA.
@misc{etde_21141098,
title = {Genetic fingerprinting of mutant rose cultivars}
author = {Kumar, S, Prasad, K V, Singh, K P, and Singh, A.P. , E-mail: kvprasad66@gmail.com}
abstractNote = {Six rose mutants evolved at the Indian Agricultural Research Institute, New Delhi from four parent cultivars were characterized based on RAPD markers. Contrary to the earlier findings our effort has conclusively proven that the RAPD markers are indeed robust tools to discern the mutants from their parents. Among 40 primers screened, 7 primers produced inconsistent banding pattern. The number of polymorphic bands varied between 4 (OPA 14) and 10 (OPA1) with an average of 6.5 bands per primer. The percentage polymorphism ranged from 62.5 (OPM 9) to 100 percent (OPA 1). Most of the primers produced monomorphic bands between parent and mutant rose cultivars. When primer OPA 2 was used a specific band of 2.5 kb was noticed in mutant cv. Pusa Urmil and cv. Pusa Abhishek but was absent in parent cv. Jantar Mantar. A polymorphic band of 750 bp was noticed in the parent Kiss of Fire and helped in differentiating the parent from its mutant when amplified with OPK 3. Primer OPS 16 produced discriminatory band of 800 bp in mutant cv. Pink Sport of Montezuma while it was absent in its parent cv. Montezuma. Another specific band of 650 bp was present in parent cv. Montezuma and absent in its mutant cv. Pink Sport of Montezuma signifying the uniqueness of the mutant. Primer OPM 5 brought out distinct polymorphism among the parent Jantar Mantar and its three mutants with absence of a specific band of 1.5 kb in the parent. The four parents and 6 mutants were divided into four distinct groups in the Dendogram constructed by UPGMA method. The most genetically similar cultivar among the 10 cultivars analyzed are Montezuma and its pink sport of Montezuma whereas Abhisarika a mutant of cv. Kiss of Fire was distinctly different and formed a separate cluster. (author)}
place = {IAEA}
year = {2008}
month = {Jul}
}