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Management of chimera and in vitro mutagenesis for development of new flower color/shape and chlorophyll variegated mutants in chrysanthemum

Conference:

Abstract

Induced mutagenesis has played a major role in the development of many new flower color/shape mutant varieties in ornamentals. The main bottleneck with vegetatively propagated plants is that the mutation appears as a chimera whether developed through bud sport or through induced mutation. The size of the mutant sector varies from a narrow streak on a petal to the entire flower and from a portion of a branch to the entire branch. When a portion of a branch or entire branch is mutated, the mutant tissue can be isolated; on the other hand, a small sector of a mutated branch or flower cannot be isolated using the available conventional propagation techniques. A novel technique has been standardized in our laboratory for the management of chimeric tissues through direct shoot regeneration from chrysanthemum florets. 'Kasturba Gandhi', a large white flowered chrysanthemum, developed few chimeric yellow florets due to spontaneous mutation. Using in vitro protocol new yellow florets were established in pure form. In vitro mutagenesis experiments were conducted treating ray florets of chrysanthemum cultivars using gamma rays. Induced chimeric yellow, white, light yellow, light mauve and dark mauve floret color sectors and chlorophyll variegation in leaves of cv. 'Maghi' (with mauve  More>>
Authors:
Datta, S.K. , E-mail: subodhskdatta@rediffmail.com; [1]  Chakrabarty, D [2] 
  1. CSIR, Madhyamgram Experimental Farm, Bose Institute, Kolkata (India)
  2. Floriculture Laboratory, National Botanical Research Institute, Lucknow (India)
Publication Date:
Jul 01, 2008
Product Type:
Conference
Report Number:
IAEA-CN-167; IAEA-CN-167-284P
Resource Relation:
Conference: International symposium on induced mutations in plants (ISIM), Vienna (Austria), 12-15 Aug 2008; Related Information: In: International symposium on induced mutations in plants (ISIM). Book of abstracts, 207 pages.
Subject:
60 APPLIED LIFE SCIENCES; BUDS; CHIMERAS; CHLOROPHYLL; FLOWERS; GAMMA RADIATION; IN VITRO; LEAVES; MUTAGENESIS; PLANTS; RADIATION INDUCED MUTANTS; SPONTANEOUS MUTATIONS; TISSUE CULTURES
OSTI ID:
21128509
Research Organizations:
Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Plant Breeding Section, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
TRN: XA0800985010140
Availability:
Available from INIS in electronic form
Submitting Site:
INIS
Size:
page(s) 124
Announcement Date:
Feb 26, 2009

Conference:

Citation Formats

Datta, S.K. , E-mail: subodhskdatta@rediffmail.com, and Chakrabarty, D. Management of chimera and in vitro mutagenesis for development of new flower color/shape and chlorophyll variegated mutants in chrysanthemum. IAEA: N. p., 2008. Web.
Datta, S.K. , E-mail: subodhskdatta@rediffmail.com, & Chakrabarty, D. Management of chimera and in vitro mutagenesis for development of new flower color/shape and chlorophyll variegated mutants in chrysanthemum. IAEA.
Datta, S.K. , E-mail: subodhskdatta@rediffmail.com, and Chakrabarty, D. 2008. "Management of chimera and in vitro mutagenesis for development of new flower color/shape and chlorophyll variegated mutants in chrysanthemum." IAEA.
@misc{etde_21128509,
title = {Management of chimera and in vitro mutagenesis for development of new flower color/shape and chlorophyll variegated mutants in chrysanthemum}
author = {Datta, S.K. , E-mail: subodhskdatta@rediffmail.com, and Chakrabarty, D}
abstractNote = {Induced mutagenesis has played a major role in the development of many new flower color/shape mutant varieties in ornamentals. The main bottleneck with vegetatively propagated plants is that the mutation appears as a chimera whether developed through bud sport or through induced mutation. The size of the mutant sector varies from a narrow streak on a petal to the entire flower and from a portion of a branch to the entire branch. When a portion of a branch or entire branch is mutated, the mutant tissue can be isolated; on the other hand, a small sector of a mutated branch or flower cannot be isolated using the available conventional propagation techniques. A novel technique has been standardized in our laboratory for the management of chimeric tissues through direct shoot regeneration from chrysanthemum florets. 'Kasturba Gandhi', a large white flowered chrysanthemum, developed few chimeric yellow florets due to spontaneous mutation. Using in vitro protocol new yellow florets were established in pure form. In vitro mutagenesis experiments were conducted treating ray florets of chrysanthemum cultivars using gamma rays. Induced chimeric yellow, white, light yellow, light mauve and dark mauve floret color sectors and chlorophyll variegation in leaves of cv. 'Maghi' (with mauve floret and green leaves) have been established in pure form. Gamma ray induced sectorial yellow florets of cv. 'Lilith' (white floret) and yellow ray florets in both the cvs. 'Purnima' (with white florets) and 'Colchi Bahar' (with red florets) have been isolated in pure form through in vitro management. Induced sectorial flower color/shape mutations in cvs. 'Puja', 'Lalima', 'Flirt', 'Maghi' and 'Sunil' have been isolated in pure form through in vitro culture. Gamma radiation procedure and tissue culture techniques have been optimized to regenerate plants from stem internodes, stem node, shoot tip and ray florets. Present technique has opened a new way for isolating new flower color/shape ornamental cultivars through retrieval of single mutated cell. (author)}
place = {IAEA}
year = {2008}
month = {Jul}
}