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Improvement of taro (Colocasia esculenta var esculenta) through in-vitro mutagenesis

Conference:

Abstract

An in-vitro mutation breeding program was implemented to improve taro (Colocasia esculenta (L). schott) for resistance to the fungus Phytophthora colocasiae. Apical shoot tips used as explants were cultured on Murashige and Skoog medium (1962) supplemented with varying concentrations of Indole-3- acetic acid, Thidiazuron (1-Phenyl-3-(1,2,3- thiadiazol-5-yl) urea (TDZ) and N6-benzylaminopurine (BA). Optimal culture initiation and multiplication was obtained on MS supplemented with 10 1mgL{sup -1}of IAA and 0.9 1mgL{sup -1}TDZ/BA at 20 1mgL{sup -1}respectively. Explants were exposed to various doses of Gamma radiation and effective mutation dose that causes 30% reduction in growth (LD{sub 30}) was found to 7.65 grays. 9 accessions of colocasia species (dasheen and eddoes type) and 2 from xanthosoma species were used for morphological and molecular characterization. 44 morphological characters were assessed and analysed with an unweighted pair group method using an arithmetic average (UPGMA). For RAPD analysis, eight 10 mer random primers were selected as they amplified more than 5 polymorphic bands. UPGMA cluster analysis using Nei and Li' distance coefficient were then performed. Both morphological and molecular analysis revealed low genetic diversity among germplasm accessions. Rapid primers screened will be useful for characterization of mutant line showing resistance to leaf blight and the micropropagation  More>>
Authors:
Seetohul, S; Maunkee, V; Gungadurdoss, M. , E-mail: areucrop@intnet.mu [1] 
  1. Agricultural Research and Extension Unit of FARC, Quatre-Bornes (Mauritius)
Publication Date:
Jul 01, 2008
Product Type:
Conference
Report Number:
IAEA-CN-167; IAEA-CN-167-196P
Resource Relation:
Conference: International symposium on induced mutations in plants (ISIM), Vienna (Austria), 12-15 Aug 2008; Related Information: In: International symposium on induced mutations in plants (ISIM). Book of abstracts, 207 pages.
Subject:
60 APPLIED LIFE SCIENCES; ACETIC ACID; FUNGI; GAMMA RADIATION; GENETIC VARIABILITY; IN VITRO; INDOLES; MUTAGENESIS; PLANT BREEDING; PLANT GROWTH; PRODUCTIVITY; RADIATION INDUCED MUTANTS; UREA
OSTI ID:
21128497
Research Organizations:
Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Plant Breeding Section, Vienna (Austria)
Country of Origin:
IAEA
Language:
English
Other Identifying Numbers:
TRN: XA0800973010128
Availability:
Available from INIS in electronic form
Submitting Site:
INIS
Size:
page(s) 119
Announcement Date:
Feb 26, 2009

Conference:

Citation Formats

Seetohul, S, Maunkee, V, and Gungadurdoss, M. , E-mail: areucrop@intnet.mu. Improvement of taro (Colocasia esculenta var esculenta) through in-vitro mutagenesis. IAEA: N. p., 2008. Web.
Seetohul, S, Maunkee, V, & Gungadurdoss, M. , E-mail: areucrop@intnet.mu. Improvement of taro (Colocasia esculenta var esculenta) through in-vitro mutagenesis. IAEA.
Seetohul, S, Maunkee, V, and Gungadurdoss, M. , E-mail: areucrop@intnet.mu. 2008. "Improvement of taro (Colocasia esculenta var esculenta) through in-vitro mutagenesis." IAEA.
@misc{etde_21128497,
title = {Improvement of taro (Colocasia esculenta var esculenta) through in-vitro mutagenesis}
author = {Seetohul, S, Maunkee, V, and Gungadurdoss, M. , E-mail: areucrop@intnet.mu}
abstractNote = {An in-vitro mutation breeding program was implemented to improve taro (Colocasia esculenta (L). schott) for resistance to the fungus Phytophthora colocasiae. Apical shoot tips used as explants were cultured on Murashige and Skoog medium (1962) supplemented with varying concentrations of Indole-3- acetic acid, Thidiazuron (1-Phenyl-3-(1,2,3- thiadiazol-5-yl) urea (TDZ) and N6-benzylaminopurine (BA). Optimal culture initiation and multiplication was obtained on MS supplemented with 10 1mgL{sup -1}of IAA and 0.9 1mgL{sup -1}TDZ/BA at 20 1mgL{sup -1}respectively. Explants were exposed to various doses of Gamma radiation and effective mutation dose that causes 30% reduction in growth (LD{sub 30}) was found to 7.65 grays. 9 accessions of colocasia species (dasheen and eddoes type) and 2 from xanthosoma species were used for morphological and molecular characterization. 44 morphological characters were assessed and analysed with an unweighted pair group method using an arithmetic average (UPGMA). For RAPD analysis, eight 10 mer random primers were selected as they amplified more than 5 polymorphic bands. UPGMA cluster analysis using Nei and Li' distance coefficient were then performed. Both morphological and molecular analysis revealed low genetic diversity among germplasm accessions. Rapid primers screened will be useful for characterization of mutant line showing resistance to leaf blight and the micropropagation methodology developed will be useful for rapid multiplication of mutants. (author)}
place = {IAEA}
year = {2008}
month = {Jul}
}