Abstract
An in-vitro mutation breeding program was implemented to improve taro (Colocasia esculenta (L). schott) for resistance to the fungus Phytophthora colocasiae. Apical shoot tips used as explants were cultured on Murashige and Skoog medium (1962) supplemented with varying concentrations of Indole-3- acetic acid, Thidiazuron (1-Phenyl-3-(1,2,3- thiadiazol-5-yl) urea (TDZ) and N6-benzylaminopurine (BA). Optimal culture initiation and multiplication was obtained on MS supplemented with 10 1mgL{sup -1}of IAA and 0.9 1mgL{sup -1}TDZ/BA at 20 1mgL{sup -1}respectively. Explants were exposed to various doses of Gamma radiation and effective mutation dose that causes 30% reduction in growth (LD{sub 30}) was found to 7.65 grays. 9 accessions of colocasia species (dasheen and eddoes type) and 2 from xanthosoma species were used for morphological and molecular characterization. 44 morphological characters were assessed and analysed with an unweighted pair group method using an arithmetic average (UPGMA). For RAPD analysis, eight 10 mer random primers were selected as they amplified more than 5 polymorphic bands. UPGMA cluster analysis using Nei and Li' distance coefficient were then performed. Both morphological and molecular analysis revealed low genetic diversity among germplasm accessions. Rapid primers screened will be useful for characterization of mutant line showing resistance to leaf blight and the micropropagation
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Seetohul, S;
Maunkee, V;
Gungadurdoss, M. , E-mail: areucrop@intnet.mu
[1]
- Agricultural Research and Extension Unit of FARC, Quatre-Bornes (Mauritius)
Citation Formats
Seetohul, S, Maunkee, V, and Gungadurdoss, M. , E-mail: areucrop@intnet.mu.
Improvement of taro (Colocasia esculenta var esculenta) through in-vitro mutagenesis.
IAEA: N. p.,
2008.
Web.
Seetohul, S, Maunkee, V, & Gungadurdoss, M. , E-mail: areucrop@intnet.mu.
Improvement of taro (Colocasia esculenta var esculenta) through in-vitro mutagenesis.
IAEA.
Seetohul, S, Maunkee, V, and Gungadurdoss, M. , E-mail: areucrop@intnet.mu.
2008.
"Improvement of taro (Colocasia esculenta var esculenta) through in-vitro mutagenesis."
IAEA.
@misc{etde_21128497,
title = {Improvement of taro (Colocasia esculenta var esculenta) through in-vitro mutagenesis}
author = {Seetohul, S, Maunkee, V, and Gungadurdoss, M. , E-mail: areucrop@intnet.mu}
abstractNote = {An in-vitro mutation breeding program was implemented to improve taro (Colocasia esculenta (L). schott) for resistance to the fungus Phytophthora colocasiae. Apical shoot tips used as explants were cultured on Murashige and Skoog medium (1962) supplemented with varying concentrations of Indole-3- acetic acid, Thidiazuron (1-Phenyl-3-(1,2,3- thiadiazol-5-yl) urea (TDZ) and N6-benzylaminopurine (BA). Optimal culture initiation and multiplication was obtained on MS supplemented with 10 1mgL{sup -1}of IAA and 0.9 1mgL{sup -1}TDZ/BA at 20 1mgL{sup -1}respectively. Explants were exposed to various doses of Gamma radiation and effective mutation dose that causes 30% reduction in growth (LD{sub 30}) was found to 7.65 grays. 9 accessions of colocasia species (dasheen and eddoes type) and 2 from xanthosoma species were used for morphological and molecular characterization. 44 morphological characters were assessed and analysed with an unweighted pair group method using an arithmetic average (UPGMA). For RAPD analysis, eight 10 mer random primers were selected as they amplified more than 5 polymorphic bands. UPGMA cluster analysis using Nei and Li' distance coefficient were then performed. Both morphological and molecular analysis revealed low genetic diversity among germplasm accessions. Rapid primers screened will be useful for characterization of mutant line showing resistance to leaf blight and the micropropagation methodology developed will be useful for rapid multiplication of mutants. (author)}
place = {IAEA}
year = {2008}
month = {Jul}
}
title = {Improvement of taro (Colocasia esculenta var esculenta) through in-vitro mutagenesis}
author = {Seetohul, S, Maunkee, V, and Gungadurdoss, M. , E-mail: areucrop@intnet.mu}
abstractNote = {An in-vitro mutation breeding program was implemented to improve taro (Colocasia esculenta (L). schott) for resistance to the fungus Phytophthora colocasiae. Apical shoot tips used as explants were cultured on Murashige and Skoog medium (1962) supplemented with varying concentrations of Indole-3- acetic acid, Thidiazuron (1-Phenyl-3-(1,2,3- thiadiazol-5-yl) urea (TDZ) and N6-benzylaminopurine (BA). Optimal culture initiation and multiplication was obtained on MS supplemented with 10 1mgL{sup -1}of IAA and 0.9 1mgL{sup -1}TDZ/BA at 20 1mgL{sup -1}respectively. Explants were exposed to various doses of Gamma radiation and effective mutation dose that causes 30% reduction in growth (LD{sub 30}) was found to 7.65 grays. 9 accessions of colocasia species (dasheen and eddoes type) and 2 from xanthosoma species were used for morphological and molecular characterization. 44 morphological characters were assessed and analysed with an unweighted pair group method using an arithmetic average (UPGMA). For RAPD analysis, eight 10 mer random primers were selected as they amplified more than 5 polymorphic bands. UPGMA cluster analysis using Nei and Li' distance coefficient were then performed. Both morphological and molecular analysis revealed low genetic diversity among germplasm accessions. Rapid primers screened will be useful for characterization of mutant line showing resistance to leaf blight and the micropropagation methodology developed will be useful for rapid multiplication of mutants. (author)}
place = {IAEA}
year = {2008}
month = {Jul}
}