Abstract
We have developed a dynamic atomic force microscopy (AFM) method based on the simultaneous excitation of the first two flexural modes of the cantilever. The instrument, called a bimodal atomic force microscope, allows us to resolve the structural components of antibodies in both monomer and pentameric forms. The instrument operates in both high and low quality factor environments, i.e., air and liquids. We show that under the same experimental conditions, bimodal AFM is more sensitive to compositional changes than amplitude modulation AFM. By using theoretical and numerical methods, we study the material contrast sensitivity as well as the forces applied on the sample during bimodal AFM operation.
MartInez, N F;
Lozano, J R;
Herruzo, E T;
Garcia, F;
Garcia, R;
[1]
Richter, C;
Sulzbach, T
[2]
- Instituto de Microelectronica de Madrid, CSIC, Isaac Newton 8, 28760 Tres Cantos, Madrid (Spain)
- NanoWorld Services GmbH, Schottkystrasse 10, 91058 Erlangen (Germany)
Citation Formats
MartInez, N F, Lozano, J R, Herruzo, E T, Garcia, F, Garcia, R, Richter, C, and Sulzbach, T.
Bimodal atomic force microscopy imaging of isolated antibodies in air and liquids.
United Kingdom: N. p.,
2008.
Web.
doi:10.1088/0957-4484/19/38/384011; COUNTRY OF INPUT: INTERNATIONAL ATOMIC ENERGY AGENCY (IAEA).
MartInez, N F, Lozano, J R, Herruzo, E T, Garcia, F, Garcia, R, Richter, C, & Sulzbach, T.
Bimodal atomic force microscopy imaging of isolated antibodies in air and liquids.
United Kingdom.
https://doi.org/10.1088/0957-4484/19/38/384011; COUNTRY OF INPUT: INTERNATIONAL ATOMIC ENERGY AGENCY (IAEA)
MartInez, N F, Lozano, J R, Herruzo, E T, Garcia, F, Garcia, R, Richter, C, and Sulzbach, T.
2008.
"Bimodal atomic force microscopy imaging of isolated antibodies in air and liquids."
United Kingdom.
https://doi.org/10.1088/0957-4484/19/38/384011; COUNTRY OF INPUT: INTERNATIONAL ATOMIC ENERGY AGENCY (IAEA).
@misc{etde_21103177,
title = {Bimodal atomic force microscopy imaging of isolated antibodies in air and liquids}
author = {MartInez, N F, Lozano, J R, Herruzo, E T, Garcia, F, Garcia, R, Richter, C, and Sulzbach, T}
abstractNote = {We have developed a dynamic atomic force microscopy (AFM) method based on the simultaneous excitation of the first two flexural modes of the cantilever. The instrument, called a bimodal atomic force microscope, allows us to resolve the structural components of antibodies in both monomer and pentameric forms. The instrument operates in both high and low quality factor environments, i.e., air and liquids. We show that under the same experimental conditions, bimodal AFM is more sensitive to compositional changes than amplitude modulation AFM. By using theoretical and numerical methods, we study the material contrast sensitivity as well as the forces applied on the sample during bimodal AFM operation.}
doi = {10.1088/0957-4484/19/38/384011; COUNTRY OF INPUT: INTERNATIONAL ATOMIC ENERGY AGENCY (IAEA)}
journal = []
issue = {38}
volume = {19}
place = {United Kingdom}
year = {2008}
month = {Sep}
}
title = {Bimodal atomic force microscopy imaging of isolated antibodies in air and liquids}
author = {MartInez, N F, Lozano, J R, Herruzo, E T, Garcia, F, Garcia, R, Richter, C, and Sulzbach, T}
abstractNote = {We have developed a dynamic atomic force microscopy (AFM) method based on the simultaneous excitation of the first two flexural modes of the cantilever. The instrument, called a bimodal atomic force microscope, allows us to resolve the structural components of antibodies in both monomer and pentameric forms. The instrument operates in both high and low quality factor environments, i.e., air and liquids. We show that under the same experimental conditions, bimodal AFM is more sensitive to compositional changes than amplitude modulation AFM. By using theoretical and numerical methods, we study the material contrast sensitivity as well as the forces applied on the sample during bimodal AFM operation.}
doi = {10.1088/0957-4484/19/38/384011; COUNTRY OF INPUT: INTERNATIONAL ATOMIC ENERGY AGENCY (IAEA)}
journal = []
issue = {38}
volume = {19}
place = {United Kingdom}
year = {2008}
month = {Sep}
}