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Study of the 6-n-propyl-2 thiouracil (PTU) as radioprotector in the thyroid cancer; Estudio del 6 propil-2 tiouracilo (PTU) como radioprotector en el tratamiento del cancer de tiroides

Journal Article:

Abstract

Full text: Introduction: Many epidemiological studies have demonstrated that exposition to high doses of external radiation increases the frequency of thyroid neoplasia, particularly during childhood and adolescence. The use of thyroid radioprotectors would be convenient to avoid tumorigenic effects in the thyroid gland when irradiation in the neck area is the only possible therapy. Objectives: To study the possible radioprotector effect of the antithyroid drug 6-n-propyl-2 thiouracil (PTU). Normal thyroid cells (FRTL-5 rat thyroid cell line) and pathologic thyroid cells (human anaplastic thyroid carcinoma cell line ARO) were cultured. The same were irradiated with a Co-60 source (1Gy/min) with a dose range between 1 and 8 Gy, in presence and in absence of PTU (1mM). Afterwards post irradiation damage was assessed through the culture formation assay using the survival fraction as effect indicator. Results: the survival fraction increase over control of both cell lines for each doses. The relation PTU vs. Control was: 2,3 and 2,7 for ARO cells and FRTL-5 respectively. The radioprotector effect of PTU is similar in both cases: if it is added 24 hs before or immediately after irradiation. As long as it has been demonstrated that the increase of tissue radioresistance can be induced through  More>>
Authors:
Perona, Marina; Dagrosa, Maria A; Pisarev, Mario A; Juvenal, Guillelrmo J; [1]  Pagotto, R; Pignataro, O; [2]  Casal, M [3] 
  1. Comision Nacional de Energia Atomica, General San Martin (Argentina). Dept. de Radiogiologia
  2. Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires (Argentina). Instituto de Biologia y Medicina Experimental
  3. Instituto Nacional de Oncologia 'Angel H. Roffo', Buenos Aires (Argentina)
Publication Date:
Jul 01, 2007
Product Type:
Journal Article
Resource Relation:
Journal Name: Medicina (Buenos Aires); Journal Volume: 67; Journal Issue: suppl.3; Conference: 52. Annual scientific meeting of the Argentine Society of Clinical Investigation; 55. Annual scientific meeting of the Argentine Society of Immunology, 52. Reunion cientifica anual de la Sociedad Argentina de Investigacion Clinica (SAIC); 55. Reunion cientifica anual de la Sociedad Argentina de Inmunologia (SAI), Mar del Plata (Argentina), 21-24 Nov 2007; Other Information: Oral communication; Abstract only
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; CARCINOMAS; COBALT 60; RADIATION DOSES; RADIOPROTECTIVE SUBSTANCES; RADIOTHERAPY; THIOURACIL; THYROID
OSTI ID:
21092215
Country of Origin:
Argentina
Language:
Spanish
Other Identifying Numbers:
Journal ID: ISSN 0025-7680; MEDCAD; TRN: AR08C0035102033
Submitting Site:
INIS
Size:
page(s) 222
Announcement Date:
Nov 12, 2008

Journal Article:

Citation Formats

Perona, Marina, Dagrosa, Maria A, Pisarev, Mario A, Juvenal, Guillelrmo J, Pagotto, R, Pignataro, O, and Casal, M. Study of the 6-n-propyl-2 thiouracil (PTU) as radioprotector in the thyroid cancer; Estudio del 6 propil-2 tiouracilo (PTU) como radioprotector en el tratamiento del cancer de tiroides. Argentina: N. p., 2007. Web.
Perona, Marina, Dagrosa, Maria A, Pisarev, Mario A, Juvenal, Guillelrmo J, Pagotto, R, Pignataro, O, & Casal, M. Study of the 6-n-propyl-2 thiouracil (PTU) as radioprotector in the thyroid cancer; Estudio del 6 propil-2 tiouracilo (PTU) como radioprotector en el tratamiento del cancer de tiroides. Argentina.
Perona, Marina, Dagrosa, Maria A, Pisarev, Mario A, Juvenal, Guillelrmo J, Pagotto, R, Pignataro, O, and Casal, M. 2007. "Study of the 6-n-propyl-2 thiouracil (PTU) as radioprotector in the thyroid cancer; Estudio del 6 propil-2 tiouracilo (PTU) como radioprotector en el tratamiento del cancer de tiroides." Argentina.
@misc{etde_21092215,
title = {Study of the 6-n-propyl-2 thiouracil (PTU) as radioprotector in the thyroid cancer; Estudio del 6 propil-2 tiouracilo (PTU) como radioprotector en el tratamiento del cancer de tiroides}
author = {Perona, Marina, Dagrosa, Maria A, Pisarev, Mario A, Juvenal, Guillelrmo J, Pagotto, R, Pignataro, O, and Casal, M}
abstractNote = {Full text: Introduction: Many epidemiological studies have demonstrated that exposition to high doses of external radiation increases the frequency of thyroid neoplasia, particularly during childhood and adolescence. The use of thyroid radioprotectors would be convenient to avoid tumorigenic effects in the thyroid gland when irradiation in the neck area is the only possible therapy. Objectives: To study the possible radioprotector effect of the antithyroid drug 6-n-propyl-2 thiouracil (PTU). Normal thyroid cells (FRTL-5 rat thyroid cell line) and pathologic thyroid cells (human anaplastic thyroid carcinoma cell line ARO) were cultured. The same were irradiated with a Co-60 source (1Gy/min) with a dose range between 1 and 8 Gy, in presence and in absence of PTU (1mM). Afterwards post irradiation damage was assessed through the culture formation assay using the survival fraction as effect indicator. Results: the survival fraction increase over control of both cell lines for each doses. The relation PTU vs. Control was: 2,3 and 2,7 for ARO cells and FRTL-5 respectively. The radioprotector effect of PTU is similar in both cases: if it is added 24 hs before or immediately after irradiation. As long as it has been demonstrated that the increase of tissue radioresistance can be induced through the stimulation of the cyclic AMP (cAMP) pathway, the levels of the second messenger were measured after the incubation of the cell lines during 5, 24, 48 and 72 hours with different concentrations of PTU (0; 0,1 mM; 1 mM and 2 mM). The PTU augmented the intracellular and extracellular cAPM levels in each treatment. After 24 hours a peak was observed in the extracellular levels incubated with PTU 1 mM of 36,97 {+-} 6,37 (fmol/{mu}g prot) vs. Control 16,67 {+-} 3,92 (fmol/{mu}g prot). The radioprotector effect was mimicked by the cAMP . Conclusion: The PTU exerts a radioprotector effect by stimulating the cAMP pathway. [Spanish] Texto completo: Introduccion: Numerosos estudios epidemiologicos han demostrado que la exposicion a altas dosis de radiacion externa incrementa la frecuencia de neoplasias de tiroides, particularmente cuando la misma ocurre en la niniez o en la adolescencia. El uso de radioprotectores de la tiroides seria de utilidad para evitar efectos tumorigenicos en la glandula cuando la radiacion ionizante en la zona del cuello es la unica terapia posible. Objetivos: Estudiar el posible efecto radioprotector de la droga antitiroidea 6 propil-2 tiouracilo (PTU). Se cultivaron celulas tiroideas normales (linea FRTL-5 de rata) y patologicas (lineas de cancer humano tiroideo anaplasico ARO). Las mismas fueron irradiadas con una fuente de 60Co (1Gy/min) en dosis variables entre 1 y 8 Gy, en presencia y ausencia de PTU (1 mM). A posteriori se evaluo el danio post radiacion mediante el ensayo de formacion de colonias tomando la fraccion de sobrevida (FS) como indicador del efecto. Resultados: La FS aumento respecto del Control en ambas lineas celulares para todas las dosis utilizadas. La relacion PTU vs. Control fue de 2,3 y 2,7 para las celulas ARO y FRTL-5 respectivamente. El efecto radioprotector del PTU es el mismo si es agregado 24 hs antes o inmediatamente post irradiacion. Dado que se ha demostrado que el aumento de la radioresistencia de los tejidos puede inducirse mediante la estimulacion de las vias del AMP ciclico (cAMP), se midieron los niveles del segundo mensajero luego de incubar las lineas celulares durante 5, 24, 48 y 72 horas con diferentes concentraciones de PTU (0; 0,1 mM; 1 mM y 2 mM). El PTU aumento los niveles intra y extracelulares del cAMP en todos los tratamientos. Se observo un pico a las 24 hs en los niveles extracelulares incubados con PTU 1 mM de 36,97 {+-} 6,37 (fmol/{mu}g de prot) vs Control 16,67 {+-} 3,92 (fmol/{mu}g de prot). El efecto radioprotector del PTU fue mimetizado por el cAMP. Conclusion: El PTU ejerce un efecto radioprotector estimulando la via del cAMP. (autor)}
journal = {Medicina (Buenos Aires)}
issue = {suppl.3}
volume = {67}
place = {Argentina}
year = {2007}
month = {Jul}
}