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Protective effects of egg-milk with MT on mice damaged by irradiation

Journal Article:

Abstract

Objective: To investigate the influences of low dose radiation (LDR) on the inhibitory effects of tumorassociated antigen peptide (TAP) extract of H-22 hepatocarcinoma in mice. Methods: Mild acid elution method was applied to prepare TAP extract (MW {<=} 3000) from tumor cell membrane. The mice were given by whole-body irradiation (WBI) with 75 mGy X-rays 12 h before immunization with TAP extract. After immunization, the cell cycle progression of the thymocytes was detected with flow cytometry, the response of the splenocytes to Con A and the percentage of T cell subsets in splenocytes were analyzed. Meantime, the tumor-inhibited effect was observed in vive. Results: The present experiment showed that the TAP extract reduced the incidence of the transplanted tumor, delayed the average appearing time and decreased the growth speed of the tumor. The response of the splenocytes to Con A increased significantly as compared with that in the control group after mice were immunized with TAP extract, but there were no changes in the cell cycle progression of thymocytes. WBI with 75 mGy X-rays given to the mice 12 h before immunization could enhance the inhibitory effects of TAP extract, the percentage of S phase increased significantly as compared with  More>>
Authors:
Hongguang, Zhao; Zhicheng, Wang; Xiang, Du; Zhe, Lu; Xiaoyan, Jiang; Shouliang, Gong; [1]  Jingyu, Shi [2] 
  1. MH Radiobiology Research Unit, School of Public Health, Jilin Univ., Changchun (China)
  2. Haerbin Chunyuan Biotechnology Development, Co., LTD, Haerbin (China)
Publication Date:
Jul 15, 2005
Product Type:
Journal Article
Resource Relation:
Journal Name: Journal of Jilin University. Medicine Edition; Journal Volume: 31; Journal Issue: 4; Other Information: 3 tabs., 9 refs
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; ANTIGENS; BIOLOGICAL FUNCTIONS; BIOLOGICAL RADIATION EFFECTS; CELL CYCLE; CELL MEMBRANES; HEPATOMAS; IMMUNITY; INHIBITION; LOW DOSE IRRADIATION; MICE; NEOPLASMS; PEPTIDES; RADIATION DOSES; SPLEEN CELLS; THYMOCYTES; TUMOR CELLS; WHOLE-BODY IRRADIATION; X RADIATION
OSTI ID:
21089299
Country of Origin:
China
Language:
Chinese
Other Identifying Numbers:
Journal ID: ISSN 1671-587X; TRN: CN0802698099509
Submitting Site:
INIS
Size:
page(s) 543-546
Announcement Date:
Nov 11, 2008

Journal Article:

Citation Formats

Hongguang, Zhao, Zhicheng, Wang, Xiang, Du, Zhe, Lu, Xiaoyan, Jiang, Shouliang, Gong, and Jingyu, Shi. Protective effects of egg-milk with MT on mice damaged by irradiation. China: N. p., 2005. Web.
Hongguang, Zhao, Zhicheng, Wang, Xiang, Du, Zhe, Lu, Xiaoyan, Jiang, Shouliang, Gong, & Jingyu, Shi. Protective effects of egg-milk with MT on mice damaged by irradiation. China.
Hongguang, Zhao, Zhicheng, Wang, Xiang, Du, Zhe, Lu, Xiaoyan, Jiang, Shouliang, Gong, and Jingyu, Shi. 2005. "Protective effects of egg-milk with MT on mice damaged by irradiation." China.
@misc{etde_21089299,
title = {Protective effects of egg-milk with MT on mice damaged by irradiation}
author = {Hongguang, Zhao, Zhicheng, Wang, Xiang, Du, Zhe, Lu, Xiaoyan, Jiang, Shouliang, Gong, and Jingyu, Shi}
abstractNote = {Objective: To investigate the influences of low dose radiation (LDR) on the inhibitory effects of tumorassociated antigen peptide (TAP) extract of H-22 hepatocarcinoma in mice. Methods: Mild acid elution method was applied to prepare TAP extract (MW {<=} 3000) from tumor cell membrane. The mice were given by whole-body irradiation (WBI) with 75 mGy X-rays 12 h before immunization with TAP extract. After immunization, the cell cycle progression of the thymocytes was detected with flow cytometry, the response of the splenocytes to Con A and the percentage of T cell subsets in splenocytes were analyzed. Meantime, the tumor-inhibited effect was observed in vive. Results: The present experiment showed that the TAP extract reduced the incidence of the transplanted tumor, delayed the average appearing time and decreased the growth speed of the tumor. The response of the splenocytes to Con A increased significantly as compared with that in the control group after mice were immunized with TAP extract, but there were no changes in the cell cycle progression of thymocytes. WBI with 75 mGy X-rays given to the mice 12 h before immunization could enhance the inhibitory effects of TAP extract, the percentage of S phase increased significantly as compared with that in the TAP extract group, and the percentage of the CD8{sup +} splenocytes increased. Conclusion: The results suggest that LDR can efficiently activate the function of immune system, and enhanced the inhibitory effects of the TAP extract. (authors)}
journal = {Journal of Jilin University. Medicine Edition}
issue = {4}
volume = {31}
place = {China}
year = {2005}
month = {Jul}
}