You need JavaScript to view this

Strong synergistic induction of CYP1A1 expression by andrographolide plus typical CYP1A inducers in mouse hepatocytes

Abstract

The effects of andrographolide, the major diterpenoid constituent of Andrographis paniculata, on the expression of cytochrome P450 superfamily 1 members, including CYP1A1, CYP1A2, and CYP1B1, as well as on aryl hydrocarbon receptor (AhR) expression in primary cultures of mouse hepatocytes were investigated in comparison with the effects of typical CYP1A inducers, including benz[a]anthracene, {beta}-naphthoflavone, and 2,3,7,8-tetrachlorodibenzo-p-dioxin. Andrographolide significantly induced the expression of CYP1A1 and CYP1A2 mRNAs in a concentration-dependent manner, as did the typical CYP1A inducers, but did not induce that of CYP1B1 or AhR. Interestingly, andrographolide plus the typical CYP1A inducers synergistically induced CYP1A1 expression, and the synergism was blocked by an AhR antagonist, resveratrol. The CYP1A1 enzyme activity showed a similar pattern of induction. This is the first report that shows that andrographolide has a potency to induce CYP1A1 enzyme and indicates that andrographolide could be a very useful compound for investigating the regulatory mechanism of the CYP1A1 induction pathway. In addition, our findings suggest preparing advice for rational administration of A. paniculata, according to its ability to induce CYP1A1 expression.
Authors:
Jaruchotikamol, Atika; [1]  Jarukamjorn, Kanokwan; [2]  Sirisangtrakul, Wanna; [1]  Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002 (Thailand)]; Sakuma, Tsutomu; Kawasaki, Yuki; [1]  Nemoto, Nobuo [1] 
  1. Department of Toxicology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194 (Japan)
  2. Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002 (Thailand)
Publication Date:
Oct 15, 2007
Product Type:
Journal Article
Resource Relation:
Journal Name: Toxicology and Applied Pharmacology; Journal Volume: 224; Journal Issue: 2; Other Information: DOI: 10.1016/j.taap.2007.07.008; PII: S0041-008X(07)00326-2; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Subject:
60 APPLIED LIFE SCIENCES; ANTHRACENE; DIOXIN; DMSO; ENZYME ACTIVITY; ENZYMES; INDIUM OXIDES; LIVER CELLS; MICE; NITRIC OXIDE; PHOSPHATES; RECEPTORS; SYNERGISM
OSTI ID:
21077823
Country of Origin:
United States
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0041-008X; TXAPA9; TRN: US08R1591090242
Availability:
Available from http://dx.doi.org/10.1016/j.taap.2007.07.008;INIS
Submitting Site:
INIS
Size:
page(s) 156-162
Announcement Date:
Oct 04, 2008

Citation Formats

Jaruchotikamol, Atika, Jarukamjorn, Kanokwan, Sirisangtrakul, Wanna, Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002 (Thailand)], Sakuma, Tsutomu, Kawasaki, Yuki, and Nemoto, Nobuo. Strong synergistic induction of CYP1A1 expression by andrographolide plus typical CYP1A inducers in mouse hepatocytes. United States: N. p., 2007. Web. doi:10.1016/j.taap.2007.07.008.
Jaruchotikamol, Atika, Jarukamjorn, Kanokwan, Sirisangtrakul, Wanna, Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002 (Thailand)], Sakuma, Tsutomu, Kawasaki, Yuki, & Nemoto, Nobuo. Strong synergistic induction of CYP1A1 expression by andrographolide plus typical CYP1A inducers in mouse hepatocytes. United States. doi:10.1016/j.taap.2007.07.008.
Jaruchotikamol, Atika, Jarukamjorn, Kanokwan, Sirisangtrakul, Wanna, Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002 (Thailand)], Sakuma, Tsutomu, Kawasaki, Yuki, and Nemoto, Nobuo. 2007. "Strong synergistic induction of CYP1A1 expression by andrographolide plus typical CYP1A inducers in mouse hepatocytes." United States. doi:10.1016/j.taap.2007.07.008. https://www.osti.gov/servlets/purl/10.1016/j.taap.2007.07.008.
@misc{etde_21077823,
title = {Strong synergistic induction of CYP1A1 expression by andrographolide plus typical CYP1A inducers in mouse hepatocytes}
author = {Jaruchotikamol, Atika, Jarukamjorn, Kanokwan, Sirisangtrakul, Wanna, Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002 (Thailand)], Sakuma, Tsutomu, Kawasaki, Yuki, and Nemoto, Nobuo}
abstractNote = {The effects of andrographolide, the major diterpenoid constituent of Andrographis paniculata, on the expression of cytochrome P450 superfamily 1 members, including CYP1A1, CYP1A2, and CYP1B1, as well as on aryl hydrocarbon receptor (AhR) expression in primary cultures of mouse hepatocytes were investigated in comparison with the effects of typical CYP1A inducers, including benz[a]anthracene, {beta}-naphthoflavone, and 2,3,7,8-tetrachlorodibenzo-p-dioxin. Andrographolide significantly induced the expression of CYP1A1 and CYP1A2 mRNAs in a concentration-dependent manner, as did the typical CYP1A inducers, but did not induce that of CYP1B1 or AhR. Interestingly, andrographolide plus the typical CYP1A inducers synergistically induced CYP1A1 expression, and the synergism was blocked by an AhR antagonist, resveratrol. The CYP1A1 enzyme activity showed a similar pattern of induction. This is the first report that shows that andrographolide has a potency to induce CYP1A1 enzyme and indicates that andrographolide could be a very useful compound for investigating the regulatory mechanism of the CYP1A1 induction pathway. In addition, our findings suggest preparing advice for rational administration of A. paniculata, according to its ability to induce CYP1A1 expression.}
doi = {10.1016/j.taap.2007.07.008}
journal = {Toxicology and Applied Pharmacology}
issue = {2}
volume = {224}
place = {United States}
year = {2007}
month = {Oct}
}