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Structural elucidation of nanocrystalline biomaterials

Abstract

Bone diseases, such as osteoporosis and osteoarthritis, are the second most prevalent health problem worldwide. In Germany approximately 5 millions people are affected by arthritis. Investigating biomineralization processes and bone molecular structure is of key importance for developing new drugs for preventing and healing bone diseases. Nuclear magnetic resonance (NMR) was the primary technique used due to its advantages in characterising poorly ordered and disordered materials. Compared to all the diffraction techniques that widely applied in structural investigations, the usefulness of NMR is independent of long range molecular order. This makes NMR an outstanding technique for studies of complex/amorphous materials. Conventional NMR experiments (single pulse, spin-echo, cross polarization (CP), etc.) as well as their modifications and high-end techniques (2D HETCOR, REDOR, etc.) were used in this work. Combining the contributions from different techniques enhances the information content of the investigations and can increase the precision of the overall conclusions. Also XRD, TEM and FTIR were applied to different extent in order to get a general idea of nanocrystalline hydroxyapatite crystallite structure. Results: - A new approach named 'Solid-state NMR spectroscopy using the lost I spin magnetization in polarization transfer experiments' has been developed for measuring the transferred I spin magnetization  More>>
Authors:
Publication Date:
Oct 23, 2008
Product Type:
Thesis/Dissertation
Report Number:
ETDE-DE-1846
Resource Relation:
Other Information: TH: Diss. (Dr.rer.nat.); Related Information: BAM-Dissertationsreihev. 34
Subject:
60 APPLIED LIFE SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; APATITES; CALCIUM PHOSPHATES; MINERALIZATION; NMR IMAGING; NMR SPECTRA; RHEUMATIC DISEASES; SKELETAL DISEASES; SPIN ECHO; TRANSMISSION ELECTRON MICROSCOPY; X-RAY DIFFRACTION
OSTI ID:
21073972
Research Organizations:
Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin (Germany); Leipzig Univ. (Germany). Fakultaet fuer Physik und Geowissenschaften
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Other: ISBN 978-3-9812072-4-8; ISSN 1613-4249; TRN: DE08GA151
Availability:
Commercial reproduction prohibited; OSTI as DE21073972
Submitting Site:
DE
Size:
137 pages
Announcement Date:
Sep 30, 2008

Citation Formats

Maltsev, S. Structural elucidation of nanocrystalline biomaterials. Germany: N. p., 2008. Web.
Maltsev, S. Structural elucidation of nanocrystalline biomaterials. Germany.
Maltsev, S. 2008. "Structural elucidation of nanocrystalline biomaterials." Germany.
@misc{etde_21073972,
title = {Structural elucidation of nanocrystalline biomaterials}
author = {Maltsev, S}
abstractNote = {Bone diseases, such as osteoporosis and osteoarthritis, are the second most prevalent health problem worldwide. In Germany approximately 5 millions people are affected by arthritis. Investigating biomineralization processes and bone molecular structure is of key importance for developing new drugs for preventing and healing bone diseases. Nuclear magnetic resonance (NMR) was the primary technique used due to its advantages in characterising poorly ordered and disordered materials. Compared to all the diffraction techniques that widely applied in structural investigations, the usefulness of NMR is independent of long range molecular order. This makes NMR an outstanding technique for studies of complex/amorphous materials. Conventional NMR experiments (single pulse, spin-echo, cross polarization (CP), etc.) as well as their modifications and high-end techniques (2D HETCOR, REDOR, etc.) were used in this work. Combining the contributions from different techniques enhances the information content of the investigations and can increase the precision of the overall conclusions. Also XRD, TEM and FTIR were applied to different extent in order to get a general idea of nanocrystalline hydroxyapatite crystallite structure. Results: - A new approach named 'Solid-state NMR spectroscopy using the lost I spin magnetization in polarization transfer experiments' has been developed for measuring the transferred I spin magnetization from abundant nuclei, which is normally lost when detecting the S spin magnetization. - A detailed investigation of nanocrystalline hydroxyapatite core was made to prove that proton environment of the phosphates units and phosphorus environment of hydroxyl units are the same as in highly crystalline hydroxyapatite sample. - Using XRD it was found that the surface of the hydroxyapatite nanocrystals is not completely disordered, as it was suggested before, but resembles the hydroxyapatite structure with HPO{sub 4}{sup 2-} (and some CO{sub 3}{sup 2-}) groups instead of PO{sub 4}{sup 3-} groups and water instead of OH{sup -} groups. - The organic-mineral interface of bone was studied in order to clarify which organic molecules are in the closest spatial proximity to the bone mineral phase and to investigate the influence of the organic matrix on the mineral formation. It was found that most probably these molecules are glycosaminoglycan rather than a protein. (orig.)}
place = {Germany}
year = {2008}
month = {Oct}
}