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Determination of optimal conditions of oxytetracyclin production from streptomyces rimosus; Optimisation de la production de l'oxytetracycline par Streptomyces rimosus

Thesis/Dissertation:

Abstract

Streptomyces rimosus is an oxytetracycline (OTC) antibiotic producing bacteria that exhibited activities against gram positive and negative bacteria. OTC is used widely not only in medicine but also in production industry. The antibiotic production of streptomyces covers a very wide range of condition. However, antibiotic producers are particularly fastidious cultivated by proper selection of media such as carbon source. In present study we have optimised conditions of OTC production (Composition of production media, p H, shaking and temperature). The results have been shown that bran barley is the optimal media for OTC production at 28C pH5.8 at 150rpm for 5 days. For antibiotic determination, OTC was extracted with different organic solvent. Thin-layer chromatography system was used for separation and identification of OTC antibiotic. High performance liquid chromatographic (HPLC) method with ultraviolet detection for the analysis of OTC is applied to the determination of OTC purification. (Author). 24 refs.
Authors:
Zouaghi, Atef [1] 
  1. Institut National des Sciences Appliquees et de Technologie (Tunisia)
Publication Date:
Jul 01, 2007
Product Type:
Thesis/Dissertation
Report Number:
INIS-TN-0004
Resource Relation:
Other Information: TH: Memoire de Projet de Fin d'Etudes (Diplome National d'Ingenieur)
Subject:
60 APPLIED LIFE SCIENCES; ANTIBIOTICS; BACTERIA; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; ORGANIC SOLVENTS; OXYTETRACYCLINE; STREPTOMYCES; THIN-LAYER CHROMATOGRAPHY
OSTI ID:
21062654
Country of Origin:
Tunisia
Language:
French
Other Identifying Numbers:
TRN: TN0800025077498
Availability:
Available from INIS in electronic form; Also available from Institut National des Sciences Appliquees et de Technologie, Tunisia
Submitting Site:
INIS
Size:
45 pages
Announcement Date:
Aug 19, 2008

Thesis/Dissertation:

Citation Formats

Zouaghi, Atef. Determination of optimal conditions of oxytetracyclin production from streptomyces rimosus; Optimisation de la production de l'oxytetracycline par Streptomyces rimosus. Tunisia: N. p., 2007. Web.
Zouaghi, Atef. Determination of optimal conditions of oxytetracyclin production from streptomyces rimosus; Optimisation de la production de l'oxytetracycline par Streptomyces rimosus. Tunisia.
Zouaghi, Atef. 2007. "Determination of optimal conditions of oxytetracyclin production from streptomyces rimosus; Optimisation de la production de l'oxytetracycline par Streptomyces rimosus." Tunisia.
@misc{etde_21062654,
title = {Determination of optimal conditions of oxytetracyclin production from streptomyces rimosus; Optimisation de la production de l'oxytetracycline par Streptomyces rimosus}
author = {Zouaghi, Atef}
abstractNote = {Streptomyces rimosus is an oxytetracycline (OTC) antibiotic producing bacteria that exhibited activities against gram positive and negative bacteria. OTC is used widely not only in medicine but also in production industry. The antibiotic production of streptomyces covers a very wide range of condition. However, antibiotic producers are particularly fastidious cultivated by proper selection of media such as carbon source. In present study we have optimised conditions of OTC production (Composition of production media, p H, shaking and temperature). The results have been shown that bran barley is the optimal media for OTC production at 28C pH5.8 at 150rpm for 5 days. For antibiotic determination, OTC was extracted with different organic solvent. Thin-layer chromatography system was used for separation and identification of OTC antibiotic. High performance liquid chromatographic (HPLC) method with ultraviolet detection for the analysis of OTC is applied to the determination of OTC purification. (Author). 24 refs.}
place = {Tunisia}
year = {2007}
month = {Jul}
}