Abstract
Previous studies have established that ethanol induces apoptosis, but the precise molecular mechanisms are currently unclear. Here, we show that 0.3-1.0% (w/v) ethanol induces apoptosis in mouse blastocysts and that resveratrol, a grape-derived phytoalexin with known antioxidant and anti-inflammatory properties, prevents ethanol-induced apoptosis and inhibition of cell proliferation. Moreover, ethanol-treated blastocysts show normal levels of implantation on culture dishes in vitro but a reduced ability to reach the later stages of embryonic development. Pretreatment with resveratrol prevented ethanol-induced disruption of embryonic development in vitro and in vivo. In an in vitro cell-based assay, we further found that ethanol increases the production of reactive oxygen species in ESC-B5 embryonic stem cells, leading to an increase in the intracellular concentrations of cytoplasmic free Ca{sup 2+} and NO, loss of mitochondrial membrane potential, mitochondrial release of cytochrome c, activation of caspase-9 and -3, and apoptosis. These changes were blocked by pretreatment with resveratrol. Based on these results, we propose a model for the protective effect of resveratrol on ethanol-induced cell injury in blastocysts and ESC-B5 cells.
Huang, L -H;
Shiao, N -H;
[1]
Hsuuw, Y -D;
[2]
Chan, W -H;
[1]
R and D Center for Membrane Technology, Chung Yuan Christian University, Taiwan (China)], E-mail: whchan@cycu.edu.tw
- Department of Bioscience Technology and Center for Nanotechnology, Chung Yuan Christian University, Chung Li 32023, Taiwan (China)
- Department of Life Science, National Pingtung University of Science and Technology, Pingtung, Taiwan (China)
Citation Formats
Huang, L -H, Shiao, N -H, Hsuuw, Y -D, Chan, W -H, and R and D Center for Membrane Technology, Chung Yuan Christian University, Taiwan (China)], E-mail: whchan@cycu.edu.tw.
Protective effects of resveratrol on ethanol-induced apoptosis in embryonic stem cells and disruption of embryonic development in mouse blastocysts.
Ireland: N. p.,
2007.
Web.
doi:10.1016/j.tox.2007.09.015.
Huang, L -H, Shiao, N -H, Hsuuw, Y -D, Chan, W -H, & R and D Center for Membrane Technology, Chung Yuan Christian University, Taiwan (China)], E-mail: whchan@cycu.edu.tw.
Protective effects of resveratrol on ethanol-induced apoptosis in embryonic stem cells and disruption of embryonic development in mouse blastocysts.
Ireland.
https://doi.org/10.1016/j.tox.2007.09.015
Huang, L -H, Shiao, N -H, Hsuuw, Y -D, Chan, W -H, and R and D Center for Membrane Technology, Chung Yuan Christian University, Taiwan (China)], E-mail: whchan@cycu.edu.tw.
2007.
"Protective effects of resveratrol on ethanol-induced apoptosis in embryonic stem cells and disruption of embryonic development in mouse blastocysts."
Ireland.
https://doi.org/10.1016/j.tox.2007.09.015.
@misc{etde_21042633,
title = {Protective effects of resveratrol on ethanol-induced apoptosis in embryonic stem cells and disruption of embryonic development in mouse blastocysts}
author = {Huang, L -H, Shiao, N -H, Hsuuw, Y -D, Chan, W -H, and R and D Center for Membrane Technology, Chung Yuan Christian University, Taiwan (China)], E-mail: whchan@cycu.edu.tw}
abstractNote = {Previous studies have established that ethanol induces apoptosis, but the precise molecular mechanisms are currently unclear. Here, we show that 0.3-1.0% (w/v) ethanol induces apoptosis in mouse blastocysts and that resveratrol, a grape-derived phytoalexin with known antioxidant and anti-inflammatory properties, prevents ethanol-induced apoptosis and inhibition of cell proliferation. Moreover, ethanol-treated blastocysts show normal levels of implantation on culture dishes in vitro but a reduced ability to reach the later stages of embryonic development. Pretreatment with resveratrol prevented ethanol-induced disruption of embryonic development in vitro and in vivo. In an in vitro cell-based assay, we further found that ethanol increases the production of reactive oxygen species in ESC-B5 embryonic stem cells, leading to an increase in the intracellular concentrations of cytoplasmic free Ca{sup 2+} and NO, loss of mitochondrial membrane potential, mitochondrial release of cytochrome c, activation of caspase-9 and -3, and apoptosis. These changes were blocked by pretreatment with resveratrol. Based on these results, we propose a model for the protective effect of resveratrol on ethanol-induced cell injury in blastocysts and ESC-B5 cells.}
doi = {10.1016/j.tox.2007.09.015}
journal = []
issue = {1-3}
volume = {242}
place = {Ireland}
year = {2007}
month = {Dec}
}
title = {Protective effects of resveratrol on ethanol-induced apoptosis in embryonic stem cells and disruption of embryonic development in mouse blastocysts}
author = {Huang, L -H, Shiao, N -H, Hsuuw, Y -D, Chan, W -H, and R and D Center for Membrane Technology, Chung Yuan Christian University, Taiwan (China)], E-mail: whchan@cycu.edu.tw}
abstractNote = {Previous studies have established that ethanol induces apoptosis, but the precise molecular mechanisms are currently unclear. Here, we show that 0.3-1.0% (w/v) ethanol induces apoptosis in mouse blastocysts and that resveratrol, a grape-derived phytoalexin with known antioxidant and anti-inflammatory properties, prevents ethanol-induced apoptosis and inhibition of cell proliferation. Moreover, ethanol-treated blastocysts show normal levels of implantation on culture dishes in vitro but a reduced ability to reach the later stages of embryonic development. Pretreatment with resveratrol prevented ethanol-induced disruption of embryonic development in vitro and in vivo. In an in vitro cell-based assay, we further found that ethanol increases the production of reactive oxygen species in ESC-B5 embryonic stem cells, leading to an increase in the intracellular concentrations of cytoplasmic free Ca{sup 2+} and NO, loss of mitochondrial membrane potential, mitochondrial release of cytochrome c, activation of caspase-9 and -3, and apoptosis. These changes were blocked by pretreatment with resveratrol. Based on these results, we propose a model for the protective effect of resveratrol on ethanol-induced cell injury in blastocysts and ESC-B5 cells.}
doi = {10.1016/j.tox.2007.09.015}
journal = []
issue = {1-3}
volume = {242}
place = {Ireland}
year = {2007}
month = {Dec}
}