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Posttranslational ruling of xanthine oxidase activity in bovine milk by its substrates

Abstract

The aims of this study were to test the hypothesis that the substrates of xanthine oxidase (XO), xanthine and hypoxanthine, are consumed while the milk is stored in the gland between milkings, and to explore how XO activity responds to bacteria commonly associated with subclinical infections in the mammary gland. Freshly secreted milk was obtained following complete evacuation of the gland and induction of milk ejection with oxytocin. In bacteria-free fresh milk xanthine and hypoxanthine were converted to uric acid within 30 min (T{sub 1/2} {approx} 10 min), which in turn provides electrons for formation of hydrogen peroxide and endows the alveolar lumen with passive protection against invading bacteria. On the other hand, the longer residence time of milk in the cistern compartment was not associated with oxidative stress as a result of XO idleness caused by exhaustion of its physiological fuels. The specific response of XO to bacteria species and the resulting bacteria-dependent nitrosative stress further demonstrates that it is part of the gland immune system.
Authors:
Silanikove, Nissim; [1]  Shapiro, Fira; [1]  Leitner, Gabriel [2] 
  1. Ruminant Physiology, Institute of Animal Science, Agricultural Research Organization, Volcani Center, P.O. Box 6, Bet Dagan 50250 (Israel)
  2. National Mastitis Reference Center, Kimron Veterinary Institute, Bet Dagan 50250 (Israel)
Publication Date:
Nov 23, 2007
Product Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 363; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2007.08.188; PII: S0006-291X(07)01939-0; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Subject:
60 APPLIED LIFE SCIENCES; BACTERIA; BIOLOGICAL STRESS; CATTLE; HYDROGEN PEROXIDE; HYPOXANTHINE; IMMUNITY; MAMMARY GLANDS; MILK; OXIDASES; OXIDATION; OXYTOCIN; SUBSTRATES; URIC ACID
OSTI ID:
21032980
Country of Origin:
United States
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0006-291X; BBRCA9; TRN: US08R0168053187
Availability:
Available from http://dx.doi.org/10.1016/j.bbrc.2007.08.188;INIS
Submitting Site:
INIS
Size:
page(s) 561-565
Announcement Date:
Jun 20, 2008

Citation Formats

Silanikove, Nissim, Shapiro, Fira, and Leitner, Gabriel. Posttranslational ruling of xanthine oxidase activity in bovine milk by its substrates. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.08.188.
Silanikove, Nissim, Shapiro, Fira, & Leitner, Gabriel. Posttranslational ruling of xanthine oxidase activity in bovine milk by its substrates. United States. doi:10.1016/j.bbrc.2007.08.188.
Silanikove, Nissim, Shapiro, Fira, and Leitner, Gabriel. 2007. "Posttranslational ruling of xanthine oxidase activity in bovine milk by its substrates." United States. doi:10.1016/j.bbrc.2007.08.188. https://www.osti.gov/servlets/purl/10.1016/j.bbrc.2007.08.188.
@misc{etde_21032980,
title = {Posttranslational ruling of xanthine oxidase activity in bovine milk by its substrates}
author = {Silanikove, Nissim, Shapiro, Fira, and Leitner, Gabriel}
abstractNote = {The aims of this study were to test the hypothesis that the substrates of xanthine oxidase (XO), xanthine and hypoxanthine, are consumed while the milk is stored in the gland between milkings, and to explore how XO activity responds to bacteria commonly associated with subclinical infections in the mammary gland. Freshly secreted milk was obtained following complete evacuation of the gland and induction of milk ejection with oxytocin. In bacteria-free fresh milk xanthine and hypoxanthine were converted to uric acid within 30 min (T{sub 1/2} {approx} 10 min), which in turn provides electrons for formation of hydrogen peroxide and endows the alveolar lumen with passive protection against invading bacteria. On the other hand, the longer residence time of milk in the cistern compartment was not associated with oxidative stress as a result of XO idleness caused by exhaustion of its physiological fuels. The specific response of XO to bacteria species and the resulting bacteria-dependent nitrosative stress further demonstrates that it is part of the gland immune system.}
doi = {10.1016/j.bbrc.2007.08.188}
journal = {Biochemical and Biophysical Research Communications}
issue = {3}
volume = {363}
place = {United States}
year = {2007}
month = {Nov}
}