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Low-level quinolone-resistance in multi-drug resistant typhoid

Journal Article:

Abstract

To find out the frequency of low-level quinolone-resistance in Multi-Drug Resistant (MDR) typhoid using nalidixic acid screening disc. Blood was obtained from suspected cases of typhoid fever and cultured in to BacT/ALERT. The positive blood cultures bottles were subcultured. The isolates were identified by colony morphology and biochemical tests using API-20E galleries. Susceptibility testing of isolates was done by modified Kirby-Bauer disc diffusion method on Muellar Hinton Agar. For the isolates, which were resistant to nalidixic acid by disc diffusion method, Minimal Inhibitory Concentrations (MICs) of ciprofloxacin and nalidixic acid were determined by using the E-test strips. Disc diffusion susceptibility tests and MICs were interpreted according to the guidelines provided by National Committee for Control Laboratory Standard (NCCLS). A total of 21(65.5%) out of 32 isolates of Salmonellae were nalidixic acid-resistant by disk diffusion method. All the nalidixic acid-resistant isolates by disc diffusion method were confirmed by MICs for both ciprofloxacin and nalidixic acid. All the nalidixic acid-resistant isolates had a ciprofloxacin MIC of 0.25-1 microg/ml (reduced susceptibility) and nalidixic acid MICs > 32 microg (resistant). Out of all Salmonella isolates, 24 (75%) were found to be MDR, and all were S. typbi. Low-level quinolone-resistance in typhoid was high in  More>>
Authors:
Mirza, S H; Khan, M A [1] 
  1. Armed Forces Inst. of Pathology, Rawalpindi (Pakistan). Dept. of Microbiolgy
Publication Date:
Jan 15, 2008
Product Type:
Journal Article
Resource Relation:
Journal Name: JCPSP. Journal of the College of Physicians and Surgeons Pakistan; Journal Volume: 18; Journal Issue: 1
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; BACTERIAL DISEASES; FEVER; MICROBIAL DRUG RESISTANCE; MORPHOLOGY; PATIENTS; SALMONELLA; TYPHOID
OSTI ID:
21031898
Country of Origin:
Pakistan
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 1022-386X; TRN: PK0800154052100
Submitting Site:
INIS
Size:
page(s) 13-16
Announcement Date:
Jun 20, 2008

Journal Article:

Citation Formats

Mirza, S H, and Khan, M A. Low-level quinolone-resistance in multi-drug resistant typhoid. Pakistan: N. p., 2008. Web.
Mirza, S H, & Khan, M A. Low-level quinolone-resistance in multi-drug resistant typhoid. Pakistan.
Mirza, S H, and Khan, M A. 2008. "Low-level quinolone-resistance in multi-drug resistant typhoid." Pakistan.
@misc{etde_21031898,
title = {Low-level quinolone-resistance in multi-drug resistant typhoid}
author = {Mirza, S H, and Khan, M A}
abstractNote = {To find out the frequency of low-level quinolone-resistance in Multi-Drug Resistant (MDR) typhoid using nalidixic acid screening disc. Blood was obtained from suspected cases of typhoid fever and cultured in to BacT/ALERT. The positive blood cultures bottles were subcultured. The isolates were identified by colony morphology and biochemical tests using API-20E galleries. Susceptibility testing of isolates was done by modified Kirby-Bauer disc diffusion method on Muellar Hinton Agar. For the isolates, which were resistant to nalidixic acid by disc diffusion method, Minimal Inhibitory Concentrations (MICs) of ciprofloxacin and nalidixic acid were determined by using the E-test strips. Disc diffusion susceptibility tests and MICs were interpreted according to the guidelines provided by National Committee for Control Laboratory Standard (NCCLS). A total of 21(65.5%) out of 32 isolates of Salmonellae were nalidixic acid-resistant by disk diffusion method. All the nalidixic acid-resistant isolates by disc diffusion method were confirmed by MICs for both ciprofloxacin and nalidixic acid. All the nalidixic acid-resistant isolates had a ciprofloxacin MIC of 0.25-1 microg/ml (reduced susceptibility) and nalidixic acid MICs > 32 microg (resistant). Out of all Salmonella isolates, 24 (75%) were found to be MDR, and all were S. typbi. Low-level quinolone-resistance in typhoid was high in this small series. Screening for nalidixic acid resistance with a 30 microg nalidixic acid disk is a reliable and cost-effective method to detect low-level fluoroquinolone resistance, especially in the developing countries. (author)}
journal = {JCPSP. Journal of the College of Physicians and Surgeons Pakistan}
issue = {1}
volume = {18}
place = {Pakistan}
year = {2008}
month = {Jan}
}