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RAPD and SSR Polymorphisms in Mutant Lines of Transgenic Wheat Mediated by Low Energy Ion Beam

Abstract

Two types of markers-random amplified polymorphic DNA (RAPD) and simple sequence repeat DNA (SSR)-have been used to characterize the genetic diversity among nine mutant lines of transgenic wheat intermediated by low energy ion beam and their four receptor cultivars. The objectives of this study were to analyze RAPD-based and SSR-based genetic variance among transgenic wheat lines and with their receptors, and to find specific genetic markers of special traits of transgenic wheat lines. 170 RAPD primers were amplified to 733 fragments in all the experimental materials. There were 121 polymorphic fragments out of the 733 fragments with a ratio of polymorphic fragments of 16.5%. 29 SSR primer pairs were amplified to 83 fragments in all the experiment materials. There were 57 polymorphic fragments out of the 83 fragments with a ratio of polymorphic fragments of 68.7%. The dendrograms were prepared based on a genetic distance matrix using the UPGMA (Unweighted Pair-group Method with Arithmetic averaging) algorithm, which corresponded well to the results of the wheat pedigree analysis and separated the 13 genotypes into four groups. Association analysis between RAPD and SSR markers with the special traits of transgenic wheat mutant lines discovered that three RAPD markers, s1, opt-16, and f14,  More>>
Authors:
Tiegu, Wang; [1]  Qunce, Huang; [1]  Weisen, Feng [2] 
  1. Henan Provincial Key Laboratory of Ion Beam Bio-Engineering, Zhengzhou University, Zhengzhou 450052 (China)
  2. Luoyang Institute of Agricultural Science, Luoyang 471022 (China)
Publication Date:
Oct 15, 2007
Product Type:
Journal Article
Resource Relation:
Journal Name: Plasma Science and Technology; Journal Volume: 9; Journal Issue: 5; Other Information: DOI: 10.1088/1009-0630/9/5/27; Country of input: International Atomic Energy Agency (IAEA)
Subject:
60 APPLIED LIFE SCIENCES; ALGORITHMS; BIOLOGICAL RADIATION EFFECTS; CLONING; DNA; GENES; GENETIC VARIABILITY; GENOTYPE; ION BEAMS; ION IMPLANTATION; RECEPTORS; WHEAT
OSTI ID:
21030620
Country of Origin:
China
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 1009-0630; TRN: CN08P3282050822
Submitting Site:
INIS
Size:
page(s) 643-648
Announcement Date:
Jun 20, 2008

Citation Formats

Tiegu, Wang, Qunce, Huang, and Weisen, Feng. RAPD and SSR Polymorphisms in Mutant Lines of Transgenic Wheat Mediated by Low Energy Ion Beam. China: N. p., 2007. Web. doi:10.1088/1009-0630/9/5/27; COUNTRY OF INPUT: INTERNATIONAL ATOMIC ENERGY AGENCY (IAEA).
Tiegu, Wang, Qunce, Huang, & Weisen, Feng. RAPD and SSR Polymorphisms in Mutant Lines of Transgenic Wheat Mediated by Low Energy Ion Beam. China. doi:10.1088/1009-0630/9/5/27; COUNTRY OF INPUT: INTERNATIONAL ATOMIC ENERGY AGENCY (IAEA).
Tiegu, Wang, Qunce, Huang, and Weisen, Feng. 2007. "RAPD and SSR Polymorphisms in Mutant Lines of Transgenic Wheat Mediated by Low Energy Ion Beam." China. doi:10.1088/1009-0630/9/5/27; COUNTRY OF INPUT: INTERNATIONAL ATOMIC ENERGY AGENCY (IAEA). https://www.osti.gov/servlets/purl/10.1088/1009-0630/9/5/27; COUNTRY OF INPUT: INTERNATIONAL ATOMIC ENERGY AGENCY (IAEA).
@misc{etde_21030620,
title = {RAPD and SSR Polymorphisms in Mutant Lines of Transgenic Wheat Mediated by Low Energy Ion Beam}
author = {Tiegu, Wang, Qunce, Huang, and Weisen, Feng}
abstractNote = {Two types of markers-random amplified polymorphic DNA (RAPD) and simple sequence repeat DNA (SSR)-have been used to characterize the genetic diversity among nine mutant lines of transgenic wheat intermediated by low energy ion beam and their four receptor cultivars. The objectives of this study were to analyze RAPD-based and SSR-based genetic variance among transgenic wheat lines and with their receptors, and to find specific genetic markers of special traits of transgenic wheat lines. 170 RAPD primers were amplified to 733 fragments in all the experimental materials. There were 121 polymorphic fragments out of the 733 fragments with a ratio of polymorphic fragments of 16.5%. 29 SSR primer pairs were amplified to 83 fragments in all the experiment materials. There were 57 polymorphic fragments out of the 83 fragments with a ratio of polymorphic fragments of 68.7%. The dendrograms were prepared based on a genetic distance matrix using the UPGMA (Unweighted Pair-group Method with Arithmetic averaging) algorithm, which corresponded well to the results of the wheat pedigree analysis and separated the 13 genotypes into four groups. Association analysis between RAPD and SSR markers with the special traits of transgenic wheat mutant lines discovered that three RAPD markers, s1, opt-16, and f14, were significantly associated with the muticate trait, while three SSR markers, Rht8 (Xgwm261), Rht-B1b, and Rht-D1b, highly associated with the dwarf trait. These markers will be useful for marker-assistant breeding and can be used as candidate markers for further gene mapping and cloning.}
doi = {10.1088/1009-0630/9/5/27; COUNTRY OF INPUT: INTERNATIONAL ATOMIC ENERGY AGENCY (IAEA)}
journal = {Plasma Science and Technology}
issue = {5}
volume = {9}
place = {China}
year = {2007}
month = {Oct}
}