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Free radical scavenger edaravone suppresses X-ray-induced apoptosis through p53 inhibition in MOLT-4 cells

Journal Article:

Abstract

Edaravone, a clinical drug used widely for the treatment of acute cerebral infarction, is reported to scavenge free radicals. In the present study, we investigated the radioprotective effect of edaravone on X-ray-induced apoptosis in MOLT-4 cells. Apoptosis was determined by the dye exclusion test, Annexin V binding assay, cleavage of caspase, and DNA fragmentation. We found that edaravone significantly suppressed the X-ray-induced apoptosis. The amount of intracellular reactive oxygen species (ROS) production was determined by the chloromethyl-2', 7'-dichlorodihydro-fluorescein diacetate system. We found that the intracellular ROS production by X-irradiation was completely suppressed by the addition of edaravone. The accumulation and phosphorylation of p53 and the expression of p21{sup WAF1}, a target protein of p53, which were induced by X-irradiation, were also suppressed by adding edaravone. We conclude that the free radical scavenger edaravone suppresses X-ray-induced apoptosis in MOLT-4 cells by inhibiting p53. (author)
Authors:
Sasano, Nakashi; Shiraishi, Kenshiro; Igaki, Hiroshi; Nakagawa, Keiichi; [1]  Enomoto, Atsushi; Hosoi, Yoshio; Matsumoto, Yoshihisa; Miyagawa, Kiyoshi; [2]  Katsumura, Yosuke [3] 
  1. Tokyo Univ., Graduate School of Medicine, Tokyo (Japan)
  2. Tokyo Univ., Faculty of Medicine, Tokyo (Japan)
  3. Tokyo Univ., Graduate School of Engineering, Tokyo (Japan)
Publication Date:
Nov 15, 2007
Product Type:
Journal Article
Resource Relation:
Journal Name: Journal of Radiation Research; Journal Volume: 48; Journal Issue: 6
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; ANIMAL CELLS; APOPTOSIS; CELL CULTURES; DNA DAMAGES; GENETIC RADIATION EFFECTS; INHIBITION; RADIATION DOSES; RADICALS; SCAVENGING; SURVIVAL CURVES; X RADIATION
OSTI ID:
20996299
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0449-3060; JRARAX; TRN: JP0800763020757
Submitting Site:
INIS
Size:
page(s) 495-503
Announcement Date:
Apr 07, 2008

Journal Article:

Citation Formats

Sasano, Nakashi, Shiraishi, Kenshiro, Igaki, Hiroshi, Nakagawa, Keiichi, Enomoto, Atsushi, Hosoi, Yoshio, Matsumoto, Yoshihisa, Miyagawa, Kiyoshi, and Katsumura, Yosuke. Free radical scavenger edaravone suppresses X-ray-induced apoptosis through p53 inhibition in MOLT-4 cells. Japan: N. p., 2007. Web. doi:10.1269/jrr.07061.
Sasano, Nakashi, Shiraishi, Kenshiro, Igaki, Hiroshi, Nakagawa, Keiichi, Enomoto, Atsushi, Hosoi, Yoshio, Matsumoto, Yoshihisa, Miyagawa, Kiyoshi, & Katsumura, Yosuke. Free radical scavenger edaravone suppresses X-ray-induced apoptosis through p53 inhibition in MOLT-4 cells. Japan. doi:10.1269/jrr.07061.
Sasano, Nakashi, Shiraishi, Kenshiro, Igaki, Hiroshi, Nakagawa, Keiichi, Enomoto, Atsushi, Hosoi, Yoshio, Matsumoto, Yoshihisa, Miyagawa, Kiyoshi, and Katsumura, Yosuke. 2007. "Free radical scavenger edaravone suppresses X-ray-induced apoptosis through p53 inhibition in MOLT-4 cells." Japan. doi:10.1269/jrr.07061. https://www.osti.gov/servlets/purl/10.1269/jrr.07061.
@misc{etde_20996299,
title = {Free radical scavenger edaravone suppresses X-ray-induced apoptosis through p53 inhibition in MOLT-4 cells}
author = {Sasano, Nakashi, Shiraishi, Kenshiro, Igaki, Hiroshi, Nakagawa, Keiichi, Enomoto, Atsushi, Hosoi, Yoshio, Matsumoto, Yoshihisa, Miyagawa, Kiyoshi, and Katsumura, Yosuke}
abstractNote = {Edaravone, a clinical drug used widely for the treatment of acute cerebral infarction, is reported to scavenge free radicals. In the present study, we investigated the radioprotective effect of edaravone on X-ray-induced apoptosis in MOLT-4 cells. Apoptosis was determined by the dye exclusion test, Annexin V binding assay, cleavage of caspase, and DNA fragmentation. We found that edaravone significantly suppressed the X-ray-induced apoptosis. The amount of intracellular reactive oxygen species (ROS) production was determined by the chloromethyl-2', 7'-dichlorodihydro-fluorescein diacetate system. We found that the intracellular ROS production by X-irradiation was completely suppressed by the addition of edaravone. The accumulation and phosphorylation of p53 and the expression of p21{sup WAF1}, a target protein of p53, which were induced by X-irradiation, were also suppressed by adding edaravone. We conclude that the free radical scavenger edaravone suppresses X-ray-induced apoptosis in MOLT-4 cells by inhibiting p53. (author)}
doi = {10.1269/jrr.07061}
journal = {Journal of Radiation Research}
issue = {6}
volume = {48}
place = {Japan}
year = {2007}
month = {Nov}
}