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Inhibitory effect of recombinant adenovirus carrying immunocaspase-3 on hepatocellular carcinoma

Abstract

Previously, Srinivasula devised a contiguous molecule (C-cp-3 or immunocaspase-3) containing the small and large subunits similar to that in the active form of caspas-3 and found C-cp-3 had similar cleavage activity to the active form of caspase-3. To search for a new clinical application of C-cp-3 to treat hepatocellular carcinoma, recombinant adenoviruses carrying the C-cp-3 and a-fetoprotein (AFP) promoter (Ad-rAFP-C-cp-3) were constructed through a bacterial homologous recombinant system. The efficiency of adenovirus-mediated gene transfer and the inhibitory effect of Ad-rAFP-C-cp-3 on the proliferation of hepatocarcinoma cells were determined by X-gal stain and MTT assay, respectively. The tumorigenicity of hepatocarcinoma cells transfected by Ad-rAFP-C-cp-3 and the antitumor effect of Ad-rAFP-C-cp-3 on transplanted tumor in nude mice were detected in vivo. The results suggested that Ad-rAFP-C-cp-3 can inhibit specifically proliferation of AFP-producing human hepatocarcinoma cells in vitro and in vivo and adenovirus-mediated C-cp-3 transfer could be used as a new method to treat human hepatocarcinoma.
Authors:
Li, Xiaohua; [1]  Fan, Rui; [1]  Zou, Xue; [1]  Gao, Lin; [1]  Jin, Haifeng; [1]  Du, Rui; [1]  Xia, Lin; [1]  Fan, Daiming [1] 
  1. State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, 17 Changle Western Road, Xi'an 710032 (China)
Publication Date:
Jun 29, 2007
Product Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 358; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2007.04.134; PII: S0006-291X(07)00880-7; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Subject:
60 APPLIED LIFE SCIENCES; ADENOVIRUS; CELL PROLIFERATION; EFFICIENCY; GENE THERAPY; GENES; HEPATOMAS; IN VITRO; IN VIVO; MICE; PROMOTERS
OSTI ID:
20991411
Country of Origin:
United States
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0006-291X; BBRCA9; TRN: US07R2206014800
Submitting Site:
INIS
Size:
page(s) 489-494
Announcement Date:
Mar 26, 2008

Citation Formats

Li, Xiaohua, Fan, Rui, Zou, Xue, Gao, Lin, Jin, Haifeng, Du, Rui, Xia, Lin, and Fan, Daiming. Inhibitory effect of recombinant adenovirus carrying immunocaspase-3 on hepatocellular carcinoma. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.04.134.
Li, Xiaohua, Fan, Rui, Zou, Xue, Gao, Lin, Jin, Haifeng, Du, Rui, Xia, Lin, & Fan, Daiming. Inhibitory effect of recombinant adenovirus carrying immunocaspase-3 on hepatocellular carcinoma. United States. doi:10.1016/j.bbrc.2007.04.134.
Li, Xiaohua, Fan, Rui, Zou, Xue, Gao, Lin, Jin, Haifeng, Du, Rui, Xia, Lin, and Fan, Daiming. 2007. "Inhibitory effect of recombinant adenovirus carrying immunocaspase-3 on hepatocellular carcinoma." United States. doi:10.1016/j.bbrc.2007.04.134. https://www.osti.gov/servlets/purl/10.1016/j.bbrc.2007.04.134.
@misc{etde_20991411,
title = {Inhibitory effect of recombinant adenovirus carrying immunocaspase-3 on hepatocellular carcinoma}
author = {Li, Xiaohua, Fan, Rui, Zou, Xue, Gao, Lin, Jin, Haifeng, Du, Rui, Xia, Lin, and Fan, Daiming}
abstractNote = {Previously, Srinivasula devised a contiguous molecule (C-cp-3 or immunocaspase-3) containing the small and large subunits similar to that in the active form of caspas-3 and found C-cp-3 had similar cleavage activity to the active form of caspase-3. To search for a new clinical application of C-cp-3 to treat hepatocellular carcinoma, recombinant adenoviruses carrying the C-cp-3 and a-fetoprotein (AFP) promoter (Ad-rAFP-C-cp-3) were constructed through a bacterial homologous recombinant system. The efficiency of adenovirus-mediated gene transfer and the inhibitory effect of Ad-rAFP-C-cp-3 on the proliferation of hepatocarcinoma cells were determined by X-gal stain and MTT assay, respectively. The tumorigenicity of hepatocarcinoma cells transfected by Ad-rAFP-C-cp-3 and the antitumor effect of Ad-rAFP-C-cp-3 on transplanted tumor in nude mice were detected in vivo. The results suggested that Ad-rAFP-C-cp-3 can inhibit specifically proliferation of AFP-producing human hepatocarcinoma cells in vitro and in vivo and adenovirus-mediated C-cp-3 transfer could be used as a new method to treat human hepatocarcinoma.}
doi = {10.1016/j.bbrc.2007.04.134}
journal = {Biochemical and Biophysical Research Communications}
issue = {2}
volume = {358}
place = {United States}
year = {2007}
month = {Jun}
}