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Isolation of 14{sub C} labelled amino acids by biosynthesis in maize plants (Zea mais L.); Obtencin de aminoacidos marcados con 14{sub C} por biosintesis en plantulas de maiz (Zea mais L)

Abstract

A method of obtaining 14{sub C} labelled amino acids by biosynthesis in maize plants which had assimilated 14CO{sub 2}, has been assayed. The plants were labelled for 60 minutes with 14{sub C}O2 produced from Ba 14{sub C}O3 (specific activity of 148 KBq/{mu}mol). An extract of the soluble compounds was obtained with 80% ethanol and the amino acids were separated from the rest of the soluble compounds by ion exchange chromatography on column of Dowex 50-X8 resin. Finally, seventeen amino acids were isolated and identified from the purified extract. The acid amino acids were separated in anionic column (Dowex 1-X8) and the neutral and basic amino acids in cationic column (Dowex 50-X4). (Author) 56 refs.
Publication Date:
Jul 01, 1983
Product Type:
Technical Report
Report Number:
JEN-536
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; AMINO ACIDS; CARBON 14 COMPOUNDS; BIOSYNTHESIS; MAIZE; ION EXCHANGE CHROMATOGRAPHY
OSTI ID:
20988456
Research Organizations:
Junta de Energia Nuclear (JEN), Madrid (Spain)
Country of Origin:
Spain
Language:
Spanish
Other Identifying Numbers:
TRN: ES08B0066
Availability:
Commercial reproduction prohibited; OSTI as DE20988456
Submitting Site:
SPN
Size:
77 pages
Announcement Date:
Apr 07, 2008

Citation Formats

Carreras, N, and Mazon, M P. Isolation of 14{sub C} labelled amino acids by biosynthesis in maize plants (Zea mais L.); Obtencin de aminoacidos marcados con 14{sub C} por biosintesis en plantulas de maiz (Zea mais L). Spain: N. p., 1983. Web.
Carreras, N, & Mazon, M P. Isolation of 14{sub C} labelled amino acids by biosynthesis in maize plants (Zea mais L.); Obtencin de aminoacidos marcados con 14{sub C} por biosintesis en plantulas de maiz (Zea mais L). Spain.
Carreras, N, and Mazon, M P. 1983. "Isolation of 14{sub C} labelled amino acids by biosynthesis in maize plants (Zea mais L.); Obtencin de aminoacidos marcados con 14{sub C} por biosintesis en plantulas de maiz (Zea mais L)." Spain.
@misc{etde_20988456,
title = {Isolation of 14{sub C} labelled amino acids by biosynthesis in maize plants (Zea mais L.); Obtencin de aminoacidos marcados con 14{sub C} por biosintesis en plantulas de maiz (Zea mais L)}
author = {Carreras, N, and Mazon, M P}
abstractNote = {A method of obtaining 14{sub C} labelled amino acids by biosynthesis in maize plants which had assimilated 14CO{sub 2}, has been assayed. The plants were labelled for 60 minutes with 14{sub C}O2 produced from Ba 14{sub C}O3 (specific activity of 148 KBq/{mu}mol). An extract of the soluble compounds was obtained with 80% ethanol and the amino acids were separated from the rest of the soluble compounds by ion exchange chromatography on column of Dowex 50-X8 resin. Finally, seventeen amino acids were isolated and identified from the purified extract. The acid amino acids were separated in anionic column (Dowex 1-X8) and the neutral and basic amino acids in cationic column (Dowex 50-X4). (Author) 56 refs.}
place = {Spain}
year = {1983}
month = {Jul}
}