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In vitro transfection of the hepatitis B virus PreS2 gene into the human hepatocarcinoma cell line HepG2 induces upregulation of human telomerase reverse transcriptase

Abstract

The preS2 domain is the minimal functional unit of transcription activators that is encoded by the Hepatitis B virus (HBV) surface (S) gene. It is present in more than one-third of the HBV-integrates in HBV induced hepatocarcinoma (HCC). To further understand the functional role of PreS2 in hepatocytes, a PreS2 expression plasmid, pcS2, was constructed and stably transfected into HepG2 cells. We conducted growth curve and colony-forming assays to study the impact of PreS2 expression on cell proliferation. Cells transfected with PreS2 proliferated more rapidly and formed colonies in soft agar. PreS2 expressing cells also induced upregulation of human telomerase reverse transcriptase (hTERT) and telomerase activation by RT-PCR and the modified TRAP assay. Blocking expression of hTERT with antisense oligonuleotide reversed the growth rate in cells stably transfected with PreS2. Our data suggest that PreS2 may increase the malignant transformation of human HCC cell line HepG2 by upregulating hTERT and inducing telomerase activation.
Authors:
Hua, Liu; [1]  Fang, Luan; [1]  Ying, Ju; [1]  Hongyu, Shen; [1]  Lifen, Gao; [1]  Xiaoyan, Wang; [1]  Suxia, Liu; [1]  Lining, Zhang; [1]  Wensheng, Sun; [1]  Chunhong, Ma; [1]  Key Laboratory for Experimental Teratology, Ministry of Education (China)]. E-mail: machunhong@sdu.edu.cn
  1. Institute of Immunology, Shandong University School of Medicine, 44 Wenhua Xi Road, Jinan 250012 (China)
Publication Date:
Apr 06, 2007
Product Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 355; Journal Issue: 2; Other Information: DOI: 10.1016/j.bbrc.2007.01.160; PII: S0006-291X(07)00233-1; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Subject:
60 APPLIED LIFE SCIENCES; AGAR; CELL PROLIFERATION; GENES; HEPATITIS; HEPATOMAS; HUMAN POPULATIONS; IN VITRO; LIVER CELLS; POLYMERASE CHAIN REACTION; TRANSCRIPTION; VIRUSES
OSTI ID:
20979869
Country of Origin:
United States
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0006-291X; BBRCA9; TRN: US07R2082008578
Submitting Site:
INIS
Size:
page(s) 379-384
Announcement Date:
Mar 07, 2008

Citation Formats

Hua, Liu, Fang, Luan, Ying, Ju, Hongyu, Shen, Lifen, Gao, Xiaoyan, Wang, Suxia, Liu, Lining, Zhang, Wensheng, Sun, Chunhong, Ma, and Key Laboratory for Experimental Teratology, Ministry of Education (China)]. E-mail: machunhong@sdu.edu.cn. In vitro transfection of the hepatitis B virus PreS2 gene into the human hepatocarcinoma cell line HepG2 induces upregulation of human telomerase reverse transcriptase. United States: N. p., 2007. Web. doi:10.1016/j.bbrc.2007.01.160.
Hua, Liu, Fang, Luan, Ying, Ju, Hongyu, Shen, Lifen, Gao, Xiaoyan, Wang, Suxia, Liu, Lining, Zhang, Wensheng, Sun, Chunhong, Ma, & Key Laboratory for Experimental Teratology, Ministry of Education (China)]. E-mail: machunhong@sdu.edu.cn. In vitro transfection of the hepatitis B virus PreS2 gene into the human hepatocarcinoma cell line HepG2 induces upregulation of human telomerase reverse transcriptase. United States. doi:10.1016/j.bbrc.2007.01.160.
Hua, Liu, Fang, Luan, Ying, Ju, Hongyu, Shen, Lifen, Gao, Xiaoyan, Wang, Suxia, Liu, Lining, Zhang, Wensheng, Sun, Chunhong, Ma, and Key Laboratory for Experimental Teratology, Ministry of Education (China)]. E-mail: machunhong@sdu.edu.cn. 2007. "In vitro transfection of the hepatitis B virus PreS2 gene into the human hepatocarcinoma cell line HepG2 induces upregulation of human telomerase reverse transcriptase." United States. doi:10.1016/j.bbrc.2007.01.160. https://www.osti.gov/servlets/purl/10.1016/j.bbrc.2007.01.160.
@misc{etde_20979869,
title = {In vitro transfection of the hepatitis B virus PreS2 gene into the human hepatocarcinoma cell line HepG2 induces upregulation of human telomerase reverse transcriptase}
author = {Hua, Liu, Fang, Luan, Ying, Ju, Hongyu, Shen, Lifen, Gao, Xiaoyan, Wang, Suxia, Liu, Lining, Zhang, Wensheng, Sun, Chunhong, Ma, and Key Laboratory for Experimental Teratology, Ministry of Education (China)]. E-mail: machunhong@sdu.edu.cn}
abstractNote = {The preS2 domain is the minimal functional unit of transcription activators that is encoded by the Hepatitis B virus (HBV) surface (S) gene. It is present in more than one-third of the HBV-integrates in HBV induced hepatocarcinoma (HCC). To further understand the functional role of PreS2 in hepatocytes, a PreS2 expression plasmid, pcS2, was constructed and stably transfected into HepG2 cells. We conducted growth curve and colony-forming assays to study the impact of PreS2 expression on cell proliferation. Cells transfected with PreS2 proliferated more rapidly and formed colonies in soft agar. PreS2 expressing cells also induced upregulation of human telomerase reverse transcriptase (hTERT) and telomerase activation by RT-PCR and the modified TRAP assay. Blocking expression of hTERT with antisense oligonuleotide reversed the growth rate in cells stably transfected with PreS2. Our data suggest that PreS2 may increase the malignant transformation of human HCC cell line HepG2 by upregulating hTERT and inducing telomerase activation.}
doi = {10.1016/j.bbrc.2007.01.160}
journal = {Biochemical and Biophysical Research Communications}
issue = {2}
volume = {355}
place = {United States}
year = {2007}
month = {Apr}
}