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Uptake of oxytetracycline and its phytotoxicity to alfalfa (Medicago sativa L.)

Abstract

A series of experiments were conducted in a hydroponic system to investigate the uptake of oxytetracycline (OTC) and its toxicity to alfalfa (Medicago sativa L.). OTC inhibited alfalfa shoot and root growth by up to 61% and 85%, respectively. The kinetics of OTC uptake could be well described by Michaelis-Menten equation with V {sub max} of 2.25 {mu}mol g{sup -1} fresh weight h{sup -1}, and K {sub m} of 0.036 mM. The uptake of OTC by alfalfa was strongly inhibited by the metabolic inhibitor, 2,4-DNP (2,4-dinitrophenol), at pH 3.5 and 6.0, but not by the aquaporin competitors, glycerol and Ag{sup +}. OTC uptake, however, was significantly inhibited by Hg{sup 2+}, suggesting that the inhibition of influx was due to general cellular stress rather than the specific action of Hg{sup 2+} on aquaporins. Results from the present study suggested that OTC uptake into alfalfa is an energy-dependent process. - Plant uptake of antibiotic oxytetracycline is energy-dependent.
Authors:
Kong, W D; [1]  Zhu, Y G; [1]  Liang, Y C; [2]  Zhang, J; [1]  Smith, F A; [3]  Yang, M [1] 
  1. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085 (China)
  2. Ministry of Agriculture Key Laboratory of Plant Nutrition and Nutrient Cycling, Institute of Soils and Fertilizers, Chinese Academy of Agricultural Sciences, Beijing 100081 (China)
  3. Soil and Land Systems, School of Earth and Environmental Sciences, University of Adelaide, DP 636, Adelaide, SA 5005 (Australia)
Publication Date:
May 15, 2007
Product Type:
Journal Article
Resource Relation:
Journal Name: Environmental Pollution (1987); Journal Volume: 147; Journal Issue: 1; Other Information: DOI: 10.1016/j.envpol.2006.08.016; PII: S0269-7491(06)00496-9; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Subject:
60 APPLIED LIFE SCIENCES; ALFALFA; BIOLOGICAL ACCUMULATION; BIOLOGICAL STRESS; DINITROPHENOL; ENERGY DEPENDENCE; GLYCEROL; MERCURY IONS; OXYTETRACYCLINE; PH VALUE; PLANT GROWTH; SILVER IONS; TOXICITY; UPTAKE
OSTI ID:
20973190
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0269-7491; ENPOEK; TRN: GB07R5757002628
Submitting Site:
GBN
Size:
page(s) 187-193
Announcement Date:
Feb 06, 2008

Citation Formats

Kong, W D, Zhu, Y G, Liang, Y C, Zhang, J, Smith, F A, and Yang, M. Uptake of oxytetracycline and its phytotoxicity to alfalfa (Medicago sativa L.). United Kingdom: N. p., 2007. Web. doi:10.1016/j.envpol.2006.08.016.
Kong, W D, Zhu, Y G, Liang, Y C, Zhang, J, Smith, F A, & Yang, M. Uptake of oxytetracycline and its phytotoxicity to alfalfa (Medicago sativa L.). United Kingdom. doi:10.1016/j.envpol.2006.08.016.
Kong, W D, Zhu, Y G, Liang, Y C, Zhang, J, Smith, F A, and Yang, M. 2007. "Uptake of oxytetracycline and its phytotoxicity to alfalfa (Medicago sativa L.)." United Kingdom. doi:10.1016/j.envpol.2006.08.016. https://www.osti.gov/servlets/purl/10.1016/j.envpol.2006.08.016.
@misc{etde_20973190,
title = {Uptake of oxytetracycline and its phytotoxicity to alfalfa (Medicago sativa L.)}
author = {Kong, W D, Zhu, Y G, Liang, Y C, Zhang, J, Smith, F A, and Yang, M}
abstractNote = {A series of experiments were conducted in a hydroponic system to investigate the uptake of oxytetracycline (OTC) and its toxicity to alfalfa (Medicago sativa L.). OTC inhibited alfalfa shoot and root growth by up to 61% and 85%, respectively. The kinetics of OTC uptake could be well described by Michaelis-Menten equation with V {sub max} of 2.25 {mu}mol g{sup -1} fresh weight h{sup -1}, and K {sub m} of 0.036 mM. The uptake of OTC by alfalfa was strongly inhibited by the metabolic inhibitor, 2,4-DNP (2,4-dinitrophenol), at pH 3.5 and 6.0, but not by the aquaporin competitors, glycerol and Ag{sup +}. OTC uptake, however, was significantly inhibited by Hg{sup 2+}, suggesting that the inhibition of influx was due to general cellular stress rather than the specific action of Hg{sup 2+} on aquaporins. Results from the present study suggested that OTC uptake into alfalfa is an energy-dependent process. - Plant uptake of antibiotic oxytetracycline is energy-dependent.}
doi = {10.1016/j.envpol.2006.08.016}
journal = {Environmental Pollution (1987)}
issue = {1}
volume = {147}
place = {United Kingdom}
year = {2007}
month = {May}
}