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Low dose radiation enhancing inhibitory effect of tumor-associated antigen peptide extract on H-22 hepatocarcinoma

Journal Article:

Abstract

Objective: To determine whether there is synergically inhibitory effect of low dose radiation (LDR) and tumor-associated antigen peptides (TAP) on tumor growth in vivo, which may provide experimental basis for potential clinical co-application of these two approaches to treat cancers. Methods: TAP extract (MW {<=}3x10{sup 6}) from tumor cell membrane was prepared with mild acid elution method , as reported. The mice were whole-bodily irradiated with 75 mGy X-rays 12 h before immunization with TAP extract. After immunization , the levels of CD3, CD69, TCR{alpha}{beta} cells and T cell subsets in the spleen were detected with FACS. The tumor growth rate was estimated, and the responses to Con A, the cytokine productions and CTL activities of splenocytes were also analyzed 7 d after immunization with TAP. Results: The present experimental results showed that the TAP extract significantly reduced the incidence of the transplanted tumor, delayed the average appearing time and decreased the growth speed of the tumor. The response of splenocytes from mice immunized with TAP extract to Con A increased significantly compared with that in the control group. Irradiation with 75 mGy X-rays 12 h before immunization further enhanced the inhibitory effect of TAP extract on tumor growth, and  More>>
Authors:
Zuyue, Sun; Jingyi, Fu; Yong, Zhao; [1]  Jianxiang, Liu; Zhibo, Fu; Xiuyi, Li; Shuzheng, Liu; Shouliang, Gong
  1. Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane Biotechnology, Inst. of Zoology, Chinese Academy of Sciences, Beijing (China)
Publication Date:
Jun 15, 2005
Product Type:
Journal Article
Resource Relation:
Journal Name: Chinese Journal of Radiological Medicine and Protection; Journal Volume: 25; Journal Issue: 3; Other Information: 1 fig., 4 tabs., 14 refs
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; CARCINOEMBRYONIC ANTIGEN; CARCINOMAS; CELL MEMBRANES; GROWTH; IMMUNITY; IN VIVO; LIVER; LIVER CELLS; LOW DOSE IRRADIATION; MICE; PEPTIDES; SPLEEN CELLS; SYNERGISM; TUMOR CELLS; WHOLE-BODY IRRADIATION; X RADIATION
OSTI ID:
20972608
Country of Origin:
China
Language:
Chinese
Other Identifying Numbers:
Journal ID: ISSN 0254-5098; ZFYZDY; TRN: CN0800171002044
Submitting Site:
INIS
Size:
page(s) 248-251
Announcement Date:
Feb 06, 2008

Journal Article:

Citation Formats

Zuyue, Sun, Jingyi, Fu, Yong, Zhao, Jianxiang, Liu, Zhibo, Fu, Xiuyi, Li, Shuzheng, Liu, and Shouliang, Gong. Low dose radiation enhancing inhibitory effect of tumor-associated antigen peptide extract on H-22 hepatocarcinoma. China: N. p., 2005. Web.
Zuyue, Sun, Jingyi, Fu, Yong, Zhao, Jianxiang, Liu, Zhibo, Fu, Xiuyi, Li, Shuzheng, Liu, & Shouliang, Gong. Low dose radiation enhancing inhibitory effect of tumor-associated antigen peptide extract on H-22 hepatocarcinoma. China.
Zuyue, Sun, Jingyi, Fu, Yong, Zhao, Jianxiang, Liu, Zhibo, Fu, Xiuyi, Li, Shuzheng, Liu, and Shouliang, Gong. 2005. "Low dose radiation enhancing inhibitory effect of tumor-associated antigen peptide extract on H-22 hepatocarcinoma." China.
@misc{etde_20972608,
title = {Low dose radiation enhancing inhibitory effect of tumor-associated antigen peptide extract on H-22 hepatocarcinoma}
author = {Zuyue, Sun, Jingyi, Fu, Yong, Zhao, Jianxiang, Liu, Zhibo, Fu, Xiuyi, Li, Shuzheng, Liu, and Shouliang, Gong}
abstractNote = {Objective: To determine whether there is synergically inhibitory effect of low dose radiation (LDR) and tumor-associated antigen peptides (TAP) on tumor growth in vivo, which may provide experimental basis for potential clinical co-application of these two approaches to treat cancers. Methods: TAP extract (MW {<=}3x10{sup 6}) from tumor cell membrane was prepared with mild acid elution method , as reported. The mice were whole-bodily irradiated with 75 mGy X-rays 12 h before immunization with TAP extract. After immunization , the levels of CD3, CD69, TCR{alpha}{beta} cells and T cell subsets in the spleen were detected with FACS. The tumor growth rate was estimated, and the responses to Con A, the cytokine productions and CTL activities of splenocytes were also analyzed 7 d after immunization with TAP. Results: The present experimental results showed that the TAP extract significantly reduced the incidence of the transplanted tumor, delayed the average appearing time and decreased the growth speed of the tumor. The response of splenocytes from mice immunized with TAP extract to Con A increased significantly compared with that in the control group. Irradiation with 75 mGy X-rays 12 h before immunization further enhanced the inhibitory effect of TAP extract on tumor growth, and increased the percentage of CD8{sup +} splenocytes. Conclusion: These results suggest that whole-body irradiation with LDR exerts a synergic inhibitory effect with TAP on tumor growth in vivo, in which enhanced cellular immune responses may be involved. (authors)}
journal = {Chinese Journal of Radiological Medicine and Protection}
issue = {3}
volume = {25}
place = {China}
year = {2005}
month = {Jun}
}