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Cadmium cytotoxicity in albino mice hepatocytes and its possible implications on tropical environments; Citotoxicidad del cadmio en hepatocitos de raton albino y sus posibles implicaciones en ambientes tropicales

Journal Article:

Abstract

Analyzed phenotypic, structural and ultrastructural alterations induced by Cd{sup +2} in hepatocytes extracted from Swiss Albino mice. Cadmium was given orally in watery solution of CdCl{sub 2} during 100 days at concentrations of 50 ppm, 100 ppm and 150 ppm. In controls, distilled water alone was used. The samples were processed with the paraffin inclusion and hematoxylin-eosin coloration techniques for light microscopy. The conventional technique was used for transmission electron microscopy . Phenotypic (size and weight differences) and physiologic changes (muscular weakness, unrest) were found; at the structural level it was noticed loss of trabecular disposition and of lobular architecture, lymphocyte agglomeration, vacuolisation, dilatation of sinusoid and central vein, among others. The ultrastructural study evidenced alterations coincident with those seen with light microscopy, which were accentuated with the increase of metal concentration: nucleolus with a high number of fibrillar centers (50 ppm); voluminous lipidic drops in the cytoplasm, loose endoplasmic rough reticulum, cytoplasmic vacuolisation, altered lysosomes and peroxisomes (100 ppm); contracted nucleis with condensed chromatin, dilatation of intracellular space and mitochondria, and loss of fibrillar areas (150 ppm). Cadmium produces a toxic effect in the hepatic cells; the effect is more severe at higher concentration, leading to cellular necrosis. (author)  More>>
Authors:
Marcano, Letty; Carruyo, Ingrid; Montiel, Xiomara; [1]  com, letty_marcano@hotmail; Faria, Clarisa de R [2] 
  1. Univ. del Zulia, Facultad Experimental de Ciencias, Dept. de Biologia (Venezuela)
  2. Univ. del Zulia, Facultad de Medicina, Catedra de Histologia, Maracaibo, Zulia (Venezuela)
Publication Date:
Jun 15, 2006
Product Type:
Journal Article
Resource Relation:
Journal Name: Revista de Biologia Tropical; Journal Volume: 54; Journal Issue: 2; Other Information: Tabs., figs., refs
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; CADMIUM; CADMIUM CHLORIDES; CONTAMINATION; ELECTRON MICROSCOPY; ENVIRONMENT; EXPERIMENTAL DATA; HISTOLOGY; LIVER CELLS; MICE; NECROSIS; OPTICAL MICROSCOPY; TOXICITY
OSTI ID:
20964132
Country of Origin:
Costa Rica
Language:
Spanish
Other Identifying Numbers:
Journal ID: ISSN 0034-7744; RBTCAP; TRN: CR0700019112118
Availability:
Also available from Biblioteca Luis Demetrio Tinoco, Universidad de Costa Rica, www.ots.cuke.edu or www.ots.ac.cr;INIS
Submitting Site:
INIS
Size:
page(s) 257-263
Announcement Date:
Jan 07, 2008

Journal Article:

Citation Formats

Marcano, Letty, Carruyo, Ingrid, Montiel, Xiomara, com, letty_marcano@hotmail, and Faria, Clarisa de R. Cadmium cytotoxicity in albino mice hepatocytes and its possible implications on tropical environments; Citotoxicidad del cadmio en hepatocitos de raton albino y sus posibles implicaciones en ambientes tropicales. Costa Rica: N. p., 2006. Web.
Marcano, Letty, Carruyo, Ingrid, Montiel, Xiomara, com, letty_marcano@hotmail, & Faria, Clarisa de R. Cadmium cytotoxicity in albino mice hepatocytes and its possible implications on tropical environments; Citotoxicidad del cadmio en hepatocitos de raton albino y sus posibles implicaciones en ambientes tropicales. Costa Rica.
Marcano, Letty, Carruyo, Ingrid, Montiel, Xiomara, com, letty_marcano@hotmail, and Faria, Clarisa de R. 2006. "Cadmium cytotoxicity in albino mice hepatocytes and its possible implications on tropical environments; Citotoxicidad del cadmio en hepatocitos de raton albino y sus posibles implicaciones en ambientes tropicales." Costa Rica.
@misc{etde_20964132,
title = {Cadmium cytotoxicity in albino mice hepatocytes and its possible implications on tropical environments; Citotoxicidad del cadmio en hepatocitos de raton albino y sus posibles implicaciones en ambientes tropicales}
author = {Marcano, Letty, Carruyo, Ingrid, Montiel, Xiomara, com, letty_marcano@hotmail, and Faria, Clarisa de R}
abstractNote = {Analyzed phenotypic, structural and ultrastructural alterations induced by Cd{sup +2} in hepatocytes extracted from Swiss Albino mice. Cadmium was given orally in watery solution of CdCl{sub 2} during 100 days at concentrations of 50 ppm, 100 ppm and 150 ppm. In controls, distilled water alone was used. The samples were processed with the paraffin inclusion and hematoxylin-eosin coloration techniques for light microscopy. The conventional technique was used for transmission electron microscopy . Phenotypic (size and weight differences) and physiologic changes (muscular weakness, unrest) were found; at the structural level it was noticed loss of trabecular disposition and of lobular architecture, lymphocyte agglomeration, vacuolisation, dilatation of sinusoid and central vein, among others. The ultrastructural study evidenced alterations coincident with those seen with light microscopy, which were accentuated with the increase of metal concentration: nucleolus with a high number of fibrillar centers (50 ppm); voluminous lipidic drops in the cytoplasm, loose endoplasmic rough reticulum, cytoplasmic vacuolisation, altered lysosomes and peroxisomes (100 ppm); contracted nucleis with condensed chromatin, dilatation of intracellular space and mitochondria, and loss of fibrillar areas (150 ppm). Cadmium produces a toxic effect in the hepatic cells; the effect is more severe at higher concentration, leading to cellular necrosis. (author) [Spanish] Se realizo un analisis de las alteraciones fenotipicas, estructurales y ultraestructurales inducidas por Cd{sup +2} en hepatocitos de raton albino suizo. El Cadmio fue suministrado via oral en solucion acuosa de CdCl{sub 2} durante 100 dias a concentraciones de 50 ppm, 100 ppm y 150 ppm. En los controles la solucion de cadmio fue sustituida por agua destilada. Las muestras fueron procesadas utilizando la tecnica de inclusion en parafina y tenidas con hematoxilina-eosina para microscopia optica y por la tecnica convencional para microscopia electronica de transmision. Se identifico cambios fenotipicos (diferencias entre talla y peso) y fisiologicos (debilidad muscular e intranquilidad); a nivel histologico, perdida de la disposicion trabecular y de la arquitectura lobulillar, focos de aglomerados linfociticos, vacuolizacion, dilatacion de sinosoides y de la vena central, entre otros. El estudio ultraestructural senala diversas alteraciones tales como: nucleolo con un elevado numero de centros fibrilares (50 ppm); voluminosas gotas de lipidos en el citoplasma, reticulo endoplasmatico rugoso distendido, vacuolizacion citoplasmatica, lisosomas y peroxisomas alterados (100 ppm); nucleos contraidos con cromatina condensada, dilatacion en el espacio intracelular y areas de perdida mitocondrial y fibrilar (150 ppm). Se sugiere que el cadmio ejerce un efecto toxico en las celulas hepaticas el cual se hace mas severo con el aumento de la concentracion, llevando a la necrosis celular. (autor)}
journal = {Revista de Biologia Tropical}
issue = {2}
volume = {54}
place = {Costa Rica}
year = {2006}
month = {Jun}
}