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Induction and recovery of morphofunctional changes in the intestine of juvenile carnivorous fish (Epinephelus coioides) upon exposure to foodborne benzo[a]pyrene

Abstract

The sublethal toxicity of dietary benzo[a]pyrene, B[a]P, on fish growth and intestinal morphofunctional changes [as measured by epithelial turnover, cell proliferation, hyperplasia, de novo crypt formation and protein absorption efficiency (i.e. expression of proton/peptide co-transporter, PepT-1, on the mucosal brush border)] were studied for the carnivorous orange-spotted grouper (Epinephelus coioides). Juvenile fish were force-fed daily with pellets containing environmentally realistic concentrations of B[a]P (dissolved in corn oil) at 0.25 {mu}g/g body weight (low-dose) and 12.5 {mu}g/g body weight (high-dose) for 4 weeks, followed by a control diet for a further 4 weeks to assess recovery. Although growth inhibition was observed in fish treated with high-dose B[a]P during the exposure period, no mortality was observed throughout the 8-week experiment. Significant hyperplasia of basal enterocytes of mucosal folds was detected shortly after 3-day exposure to the high-dose B[a]P. Moreover, a faster epithelial turnover was measured in the high-dose B[a]P exposed fish at exposure week 1, which was followed by an increase of basal cell proliferation and a reduction of PepT-1 expression at exposure week 2. The formation of de novo crypts, resemblance to the cancer predisposition syndrome 'juvenile polyposis', was significantly higher in the intestine of high-dose treated fish as compared to  More>>
Authors:
Yuen, Bonny B.H.; [1]  Nicholas School of the Environment, Duke University, Durham, NC (United States)]; Wong, Chris K.C.; [2]  Woo, N Y.S.; [3]  Au, Doris W.T. [1] 
  1. Centre for Coastal Pollution and Conservation, Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong (China)
  2. Department of Biology, Baptist University of Hong Kong, Waterloo Road, Kowloon, Hong Kong (China)
  3. Department of Biology, Chinese University of Hong Kong, New Territory, Hong Kong (China)
Publication Date:
May 15, 2007
Product Type:
Journal Article
Resource Relation:
Journal Name: Aquatic Toxicology; Journal Volume: 82; Journal Issue: 3; Other Information: DOI: 10.1016/j.aquatox.2007.02.010; PII: S0166-445X(07)00076-8; Copyright (c) 2007 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Subject:
60 APPLIED LIFE SCIENCES; ABSORPTION; CELL PROLIFERATION; CORN OIL; DOSES; INTESTINES; JUVENILES; MORTALITY; NEOPLASMS; PELLETS; PEPTIDES; PYRENE; TOXICITY
OSTI ID:
20953100
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0166-445X; AQTODG; TRN: NL07S2570104375
Submitting Site:
NLN
Size:
page(s) 181-194
Announcement Date:
Dec 21, 2007

Citation Formats

Yuen, Bonny B.H., Nicholas School of the Environment, Duke University, Durham, NC (United States)], Wong, Chris K.C., Woo, N Y.S., and Au, Doris W.T. Induction and recovery of morphofunctional changes in the intestine of juvenile carnivorous fish (Epinephelus coioides) upon exposure to foodborne benzo[a]pyrene. Netherlands: N. p., 2007. Web. doi:10.1016/j.aquatox.2007.02.010.
Yuen, Bonny B.H., Nicholas School of the Environment, Duke University, Durham, NC (United States)], Wong, Chris K.C., Woo, N Y.S., & Au, Doris W.T. Induction and recovery of morphofunctional changes in the intestine of juvenile carnivorous fish (Epinephelus coioides) upon exposure to foodborne benzo[a]pyrene. Netherlands. doi:10.1016/j.aquatox.2007.02.010.
Yuen, Bonny B.H., Nicholas School of the Environment, Duke University, Durham, NC (United States)], Wong, Chris K.C., Woo, N Y.S., and Au, Doris W.T. 2007. "Induction and recovery of morphofunctional changes in the intestine of juvenile carnivorous fish (Epinephelus coioides) upon exposure to foodborne benzo[a]pyrene." Netherlands. doi:10.1016/j.aquatox.2007.02.010. https://www.osti.gov/servlets/purl/10.1016/j.aquatox.2007.02.010.
@misc{etde_20953100,
title = {Induction and recovery of morphofunctional changes in the intestine of juvenile carnivorous fish (Epinephelus coioides) upon exposure to foodborne benzo[a]pyrene}
author = {Yuen, Bonny B.H., Nicholas School of the Environment, Duke University, Durham, NC (United States)], Wong, Chris K.C., Woo, N Y.S., and Au, Doris W.T.}
abstractNote = {The sublethal toxicity of dietary benzo[a]pyrene, B[a]P, on fish growth and intestinal morphofunctional changes [as measured by epithelial turnover, cell proliferation, hyperplasia, de novo crypt formation and protein absorption efficiency (i.e. expression of proton/peptide co-transporter, PepT-1, on the mucosal brush border)] were studied for the carnivorous orange-spotted grouper (Epinephelus coioides). Juvenile fish were force-fed daily with pellets containing environmentally realistic concentrations of B[a]P (dissolved in corn oil) at 0.25 {mu}g/g body weight (low-dose) and 12.5 {mu}g/g body weight (high-dose) for 4 weeks, followed by a control diet for a further 4 weeks to assess recovery. Although growth inhibition was observed in fish treated with high-dose B[a]P during the exposure period, no mortality was observed throughout the 8-week experiment. Significant hyperplasia of basal enterocytes of mucosal folds was detected shortly after 3-day exposure to the high-dose B[a]P. Moreover, a faster epithelial turnover was measured in the high-dose B[a]P exposed fish at exposure week 1, which was followed by an increase of basal cell proliferation and a reduction of PepT-1 expression at exposure week 2. The formation of de novo crypts, resemblance to the cancer predisposition syndrome 'juvenile polyposis', was significantly higher in the intestine of high-dose treated fish as compared to the control at exposure week 2 and onwards. Abnormal cytoplasmic extrusions were frequently observed in mucosal folds of high-dose fish at exposure week 4. In the low-dose treatment group, only the expression of PepT-1 was significantly reduced at exposure week 2 and an early adaptive response was observed at exposure week 4. Despite all these intestinal disturbances were reversible in fish upon the abatement to dietary B[a]P (within 1-4 weeks), environmental realistic levels of foodborne B[a]P could induce sublethal toxicity to E. coioides, and probably impose potential risk to the marine environment. As an increase in de novo crypts was observed towards the end of the 4-week depuration period, the long-term impacts of dietary B[a]P on fish intestinal neoplasm formation worth further investigation.}
doi = {10.1016/j.aquatox.2007.02.010}
journal = {Aquatic Toxicology}
issue = {3}
volume = {82}
place = {Netherlands}
year = {2007}
month = {May}
}