Abstract
Radiation induced fibrosarcoma (RIF) cells did not show the adaptive response, i.e. a reduced effect from a higher challenging dose (4 Gy) of a radiation when a priming dose (1 cGy) had been applied 4 or 7 hrs earlier, but its thermoresistant clone (TR) did. Since inducible HSP70 expression was different between two cell lines, the role of inducible HSP70 in adaptive response was examined. When inducible hsp70 was transfected to RIF cells, the adaptive response was acquired. Inducible hsp70 transfected NIH3T3 mouse embryo cells also conferred radioresistance when detected by clonogenic survival, [3H]-thymidine incoporation, and ELISA cell death detection kit, as well as increased tendency of induction adaptive response. Interestingly, basal levels of Ca2{sup +}-dependent and Ca2{sup +}-independent PKC activities were increased by inducible HSP70, when compared to those of control vector cells. Irradiation of g-ray induced PKC activation within minutes in control vector cells, while it did not by inducible HSP70. Cellular redistribution to particulate fraction of PKCa, d and z by g-rays was not detected either. Furthermore, radioresistance by inducible HSP70 was disappeared by pre-treatment of PKC inhibitors, 1-(5-isoquinolinesulfomyI)-2-methylpepirazine (H7), prolonged treatment of 12-O-tetradecanoylphorbol-13-acetate (TPA), and GF109203X in clonogenic survival assay. Taken together, these data suggest that
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Lee, Su Jae;
Kim, Tae Hwan;
Cho, Chul Koo;
Yoo, Seong Yul;
Lee, Yun Sil
[1]
- Korea Cancer Center Hospital, Seoul (Korea, Republic of)
Citation Formats
Lee, Su Jae, Kim, Tae Hwan, Cho, Chul Koo, Yoo, Seong Yul, and Lee, Yun Sil.
Inducible heat shock protein 70 (HSP70) Involves in radioadaptive response.
Korea, Republic of: N. p.,
1999.
Web.
Lee, Su Jae, Kim, Tae Hwan, Cho, Chul Koo, Yoo, Seong Yul, & Lee, Yun Sil.
Inducible heat shock protein 70 (HSP70) Involves in radioadaptive response.
Korea, Republic of.
Lee, Su Jae, Kim, Tae Hwan, Cho, Chul Koo, Yoo, Seong Yul, and Lee, Yun Sil.
1999.
"Inducible heat shock protein 70 (HSP70) Involves in radioadaptive response."
Korea, Republic of.
@misc{etde_20932250,
title = {Inducible heat shock protein 70 (HSP70) Involves in radioadaptive response}
author = {Lee, Su Jae, Kim, Tae Hwan, Cho, Chul Koo, Yoo, Seong Yul, and Lee, Yun Sil}
abstractNote = {Radiation induced fibrosarcoma (RIF) cells did not show the adaptive response, i.e. a reduced effect from a higher challenging dose (4 Gy) of a radiation when a priming dose (1 cGy) had been applied 4 or 7 hrs earlier, but its thermoresistant clone (TR) did. Since inducible HSP70 expression was different between two cell lines, the role of inducible HSP70 in adaptive response was examined. When inducible hsp70 was transfected to RIF cells, the adaptive response was acquired. Inducible hsp70 transfected NIH3T3 mouse embryo cells also conferred radioresistance when detected by clonogenic survival, [3H]-thymidine incoporation, and ELISA cell death detection kit, as well as increased tendency of induction adaptive response. Interestingly, basal levels of Ca2{sup +}-dependent and Ca2{sup +}-independent PKC activities were increased by inducible HSP70, when compared to those of control vector cells. Irradiation of g-ray induced PKC activation within minutes in control vector cells, while it did not by inducible HSP70. Cellular redistribution to particulate fraction of PKCa, d and z by g-rays was not detected either. Furthermore, radioresistance by inducible HSP70 was disappeared by pre-treatment of PKC inhibitors, 1-(5-isoquinolinesulfomyI)-2-methylpepirazine (H7), prolonged treatment of 12-O-tetradecanoylphorbol-13-acetate (TPA), and GF109203X in clonogenic survival assay. Taken together, these data suggest that inducible HSP70-induced radioresistance is associated with its elevated level of PKC activity.}
place = {Korea, Republic of}
year = {1999}
month = {Jul}
}
title = {Inducible heat shock protein 70 (HSP70) Involves in radioadaptive response}
author = {Lee, Su Jae, Kim, Tae Hwan, Cho, Chul Koo, Yoo, Seong Yul, and Lee, Yun Sil}
abstractNote = {Radiation induced fibrosarcoma (RIF) cells did not show the adaptive response, i.e. a reduced effect from a higher challenging dose (4 Gy) of a radiation when a priming dose (1 cGy) had been applied 4 or 7 hrs earlier, but its thermoresistant clone (TR) did. Since inducible HSP70 expression was different between two cell lines, the role of inducible HSP70 in adaptive response was examined. When inducible hsp70 was transfected to RIF cells, the adaptive response was acquired. Inducible hsp70 transfected NIH3T3 mouse embryo cells also conferred radioresistance when detected by clonogenic survival, [3H]-thymidine incoporation, and ELISA cell death detection kit, as well as increased tendency of induction adaptive response. Interestingly, basal levels of Ca2{sup +}-dependent and Ca2{sup +}-independent PKC activities were increased by inducible HSP70, when compared to those of control vector cells. Irradiation of g-ray induced PKC activation within minutes in control vector cells, while it did not by inducible HSP70. Cellular redistribution to particulate fraction of PKCa, d and z by g-rays was not detected either. Furthermore, radioresistance by inducible HSP70 was disappeared by pre-treatment of PKC inhibitors, 1-(5-isoquinolinesulfomyI)-2-methylpepirazine (H7), prolonged treatment of 12-O-tetradecanoylphorbol-13-acetate (TPA), and GF109203X in clonogenic survival assay. Taken together, these data suggest that inducible HSP70-induced radioresistance is associated with its elevated level of PKC activity.}
place = {Korea, Republic of}
year = {1999}
month = {Jul}
}