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Influence of ionizing radiation on Trypanosoma cruzi; Influencia da radiacao ionizante sobre o Trypanosoma cruzi

Abstract

Chagas's disease is one of the major public health problems in South America, promoting high prejudice to the local population. Despite the massive efforts to control it, this disease has no cure and presents puzzling unsolved questions. Considering that many researchers have used ionizing radiation to modify protozoans or biomolecules, we investigated the immunological response aspects of susceptible and resistant mice using irradiated parasites. Low radiation doses preserved the reproductive and invasive capacities of the parasite. Both susceptible and resistant animals, after immunization with irradiated parasites produced specific antibodies. After a challenge, the animals presented low parasitaemia, excepting those immunized with the antigen irradiated with higher doses. Using low radiation doses, we were able to selectively isolate trypomastigotes, leading to an improvement in the quality of the immune response, as previously reported when performing complement system assays. These data highlight the importance of selecting trypomastigote forms for immunization against T. cruz; and point towards ionizing radiation as an alternative to achieve this selection, since when this procedure is performed using complement, the subsequent steps are impaired by the difficulties to remove this component from the system. (author)
Publication Date:
Jul 01, 2006
Product Type:
Thesis/Dissertation
Report Number:
INIS-BR-4132
Resource Relation:
Other Information: TH: Dissertacao (M.Sc.); refs., 20 figs., 1 tab
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; BIOLOGICAL RADIATION EFFECTS; COBALT 60; DOSE-RESPONSE RELATIONSHIPS; ENZYME IMMUNOASSAY; EXPERIMENTAL DATA; GAMMA RADIATION; IMMUNE REACTIONS; MICE; RADIATION DOSES; TRYPANOSOMA; TRYPANOSOMIASIS
OSTI ID:
20902527
Research Organizations:
Instituto de Pesquisas Energeticas e Nucleares (IPEN/CNEN-SP), Sao Paulo, SP (Brazil)
Country of Origin:
Brazil
Language:
Portuguese
Other Identifying Numbers:
TRN: BR0746383065066
Availability:
Available from INIS in electronic form; Also available from the Library of the Brazilian Nuclear and Energy Research Institute (CNEN/IPEN-SP), Sao Paulo, SP
Submitting Site:
BRN
Size:
74 pages
Announcement Date:
Aug 28, 2007

Citation Formats

Szarota, Rosa Maria. Influence of ionizing radiation on Trypanosoma cruzi; Influencia da radiacao ionizante sobre o Trypanosoma cruzi. Brazil: N. p., 2006. Web.
Szarota, Rosa Maria. Influence of ionizing radiation on Trypanosoma cruzi; Influencia da radiacao ionizante sobre o Trypanosoma cruzi. Brazil.
Szarota, Rosa Maria. 2006. "Influence of ionizing radiation on Trypanosoma cruzi; Influencia da radiacao ionizante sobre o Trypanosoma cruzi." Brazil.
@misc{etde_20902527,
title = {Influence of ionizing radiation on Trypanosoma cruzi; Influencia da radiacao ionizante sobre o Trypanosoma cruzi}
author = {Szarota, Rosa Maria}
abstractNote = {Chagas's disease is one of the major public health problems in South America, promoting high prejudice to the local population. Despite the massive efforts to control it, this disease has no cure and presents puzzling unsolved questions. Considering that many researchers have used ionizing radiation to modify protozoans or biomolecules, we investigated the immunological response aspects of susceptible and resistant mice using irradiated parasites. Low radiation doses preserved the reproductive and invasive capacities of the parasite. Both susceptible and resistant animals, after immunization with irradiated parasites produced specific antibodies. After a challenge, the animals presented low parasitaemia, excepting those immunized with the antigen irradiated with higher doses. Using low radiation doses, we were able to selectively isolate trypomastigotes, leading to an improvement in the quality of the immune response, as previously reported when performing complement system assays. These data highlight the importance of selecting trypomastigote forms for immunization against T. cruz; and point towards ionizing radiation as an alternative to achieve this selection, since when this procedure is performed using complement, the subsequent steps are impaired by the difficulties to remove this component from the system. (author)}
place = {Brazil}
year = {2006}
month = {Jul}
}