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Radiosensitivity of three species of ground orchids (Spathoglottis plicata, S. kimballiana var. angustifolia and S. tomentosa) to acute gamma radiation

Abstract

A radiosensitivity study coupled with tissue culture technique was conducted as preliminary to mutation breeding of the three species of ground orchids (Spathoglottis plicata, S. kimballiana var. angustifolia, and S. tomentosa). It aimed to compare the effects of varying dose levels of gamma radiation applied to the germinated embryos (protocorms) of the three species. Also it sought to determine the lethal dose of gamma radiation on the three species and to determine their optimum dose or the dose level that will lead to production of more mutants. The protocorms of the three species were irradiated at 10 Gy, 20 Gy, 30 Gy, 40 Gy, and 50 Gy dose levels of gamma radiation. Results of the study showed that as the dose level administered increases, percent mortality of seedlings also increases. Further, seedling height, number of roots and root length decreases. However, there was an increase in number of leaves at certain dose levels due to the emergence of furcations, but further increase in the dose levels of radiation decreases the number of leaves.Furthermore, some qualitative characters such as albinism, pigmentation, forked leaves, furcations, and multiple branching came out as responses to gamma radiation. It further shows that the three species  More>>
Publication Date:
Apr 15, 2007
Product Type:
Thesis/Dissertation
Report Number:
INIS-PH-031
Resource Relation:
Other Information: TH: B.S. Agriculture; 34 refs.; 26 figs.; 16 tabs
Subject:
60 APPLIED LIFE SCIENCES; EMBRYOS; EXPERIMENTAL DATA; GAMMA RADIATION; LETHAL DOSES; MUTATIONS; ORNAMENTAL PLANTS; PLANT BREEDING; PLANTS; RADIOSENSITIVITY; RADIOSENSITIVITY EFFECTS; TISSUE CULTURES
OSTI ID:
20899220
Research Organizations:
Central Luzon State University, Science City of Munoz, Nueva Ecija (Philippines)
Country of Origin:
Philippines
Language:
English
Other Identifying Numbers:
TRN: PH0700003061417
Availability:
Available from INIS in electronic form; Also available from PNRI library
Submitting Site:
INIS
Size:
83 pages
Announcement Date:
Aug 28, 2007

Citation Formats

Gonzales, Marcial Alvaran. Radiosensitivity of three species of ground orchids (Spathoglottis plicata, S. kimballiana var. angustifolia and S. tomentosa) to acute gamma radiation. Philippines: N. p., 2007. Web.
Gonzales, Marcial Alvaran. Radiosensitivity of three species of ground orchids (Spathoglottis plicata, S. kimballiana var. angustifolia and S. tomentosa) to acute gamma radiation. Philippines.
Gonzales, Marcial Alvaran. 2007. "Radiosensitivity of three species of ground orchids (Spathoglottis plicata, S. kimballiana var. angustifolia and S. tomentosa) to acute gamma radiation." Philippines.
@misc{etde_20899220,
title = {Radiosensitivity of three species of ground orchids (Spathoglottis plicata, S. kimballiana var. angustifolia and S. tomentosa) to acute gamma radiation}
author = {Gonzales, Marcial Alvaran}
abstractNote = {A radiosensitivity study coupled with tissue culture technique was conducted as preliminary to mutation breeding of the three species of ground orchids (Spathoglottis plicata, S. kimballiana var. angustifolia, and S. tomentosa). It aimed to compare the effects of varying dose levels of gamma radiation applied to the germinated embryos (protocorms) of the three species. Also it sought to determine the lethal dose of gamma radiation on the three species and to determine their optimum dose or the dose level that will lead to production of more mutants. The protocorms of the three species were irradiated at 10 Gy, 20 Gy, 30 Gy, 40 Gy, and 50 Gy dose levels of gamma radiation. Results of the study showed that as the dose level administered increases, percent mortality of seedlings also increases. Further, seedling height, number of roots and root length decreases. However, there was an increase in number of leaves at certain dose levels due to the emergence of furcations, but further increase in the dose levels of radiation decreases the number of leaves.Furthermore, some qualitative characters such as albinism, pigmentation, forked leaves, furcations, and multiple branching came out as responses to gamma radiation. It further shows that the three species have varied radiosensitivity as affected by their individual phenotype. It was found that S. kimballiana var. angustifolia was the least radiosensitive among the species, and could have a great potential for a wide array of genetic variations due to the observed emergence of more morphological mutations that came out as effect of gamma radiation. (Author)}
place = {Philippines}
year = {2007}
month = {Apr}
}