You need JavaScript to view this

Effect of 8-MOP plus UVA treatment on survival and repair of plasmid pBR322; Efecto del tratamiento con 8-MOP mas UVA en la supervivencia y reparacion de pBR322

Abstract

We have studied the lethality produced in pBR322 DNA after PUVA treatment (8-MOP+UVA). As recipients, we used a collection of E. coli strains differing in their repair capacities and analysed the involvement of several DNA repair pathways in the removal of plasmid lesions. We have also studied the effect of UVA radiation alone, in order to determine more precisely the effect attributable only to psoralen molecules. Results showed a strong lethal effect derived from PUVA treatment; however, some plasmid recovery was achieved in bacterial hosts proficient in Excision repair and SOS repair. Another repair pathway, only detectable at high density of lesions, appeared to be relevant for the removal of 8-MOP:DNA adducts.(Author) 11 refs.
Publication Date:
Jul 01, 1991
Product Type:
Technical Report
Report Number:
CIEMAT-667
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; DNA; DNA REPAIR; DNA ADDUCTS; ESCHERICHIA COLI; PLASMIDS; NEAR ULTRAVIOLET RADIATION; PSORALEN; LETHAL DOSES; SURVIVAL TIME
OSTI ID:
20897446
Research Organizations:
Centro de Investigaciones Energeticas Medioambientales y Tecnologicas (CIEMAT), Madrid (Spain)
Country of Origin:
Spain
Language:
Spanish
Other Identifying Numbers:
TRN: ES07B0344
Availability:
Commercial reproduction prohibited; OSTI as DE20897446
Submitting Site:
SPN
Size:
100 pages
Announcement Date:
Aug 10, 2007

Citation Formats

Bauluz, C, and Vidania, R de. Effect of 8-MOP plus UVA treatment on survival and repair of plasmid pBR322; Efecto del tratamiento con 8-MOP mas UVA en la supervivencia y reparacion de pBR322. Spain: N. p., 1991. Web.
Bauluz, C, & Vidania, R de. Effect of 8-MOP plus UVA treatment on survival and repair of plasmid pBR322; Efecto del tratamiento con 8-MOP mas UVA en la supervivencia y reparacion de pBR322. Spain.
Bauluz, C, and Vidania, R de. 1991. "Effect of 8-MOP plus UVA treatment on survival and repair of plasmid pBR322; Efecto del tratamiento con 8-MOP mas UVA en la supervivencia y reparacion de pBR322." Spain.
@misc{etde_20897446,
title = {Effect of 8-MOP plus UVA treatment on survival and repair of plasmid pBR322; Efecto del tratamiento con 8-MOP mas UVA en la supervivencia y reparacion de pBR322}
author = {Bauluz, C, and Vidania, R de}
abstractNote = {We have studied the lethality produced in pBR322 DNA after PUVA treatment (8-MOP+UVA). As recipients, we used a collection of E. coli strains differing in their repair capacities and analysed the involvement of several DNA repair pathways in the removal of plasmid lesions. We have also studied the effect of UVA radiation alone, in order to determine more precisely the effect attributable only to psoralen molecules. Results showed a strong lethal effect derived from PUVA treatment; however, some plasmid recovery was achieved in bacterial hosts proficient in Excision repair and SOS repair. Another repair pathway, only detectable at high density of lesions, appeared to be relevant for the removal of 8-MOP:DNA adducts.(Author) 11 refs.}
place = {Spain}
year = {1991}
month = {Jul}
}