Abstract
Objective: To investigate a new agent {sup 153}Sm-ethylene diamine tetramethylene phosphonic acid (EDTMP)-nano-Hydroxyapatite (nano-HA) and the in vitro and in vivo performance of three derivatives of this agent aiming to explore a new radiopharmaceutical for targeting therapy of bone metastases. Methods: The nano-HA was synthesized by collosol-gelatum method, and was evaluated by transmission electron microscope (TEM) and X-ray diffraction (XRD). {sup 153}Sm was produced with a high specific activity and excellent radionuclidic purity; adopting independent variable method, that nano-HA being labeled with {sup 153}Sm by EDTMP transfer ligands in the best condition was investigated. The stability of {sup 153}Sm-EDTMP-nano-HA in vitro was studied. {sup 153}Sm-EDTMP-nano-HA was injected into normal rabbits for in vivo study. Both SMMC-7721 and MCF-7 cell lines were divided into three groups, treated with nano-HA, {sup 153}Sm-EDTMP and {sup 153}Sm-EDTMP-nano-HA, respectively, and the survival rate was measured by mono-nuclear cell direct cytotoxicity assay method. Results: The detection with TEM, XRD showed that nano-HA consisted of needle-like microcrystals. The labelling rate of {sup 153}Sm-EDTMP-nano-HA was more than 95%, and was extremely stable in vitro. The scanning of normal rabbits showed that the skeletal system was clear , but other systems, for example, liver, spleen and kidney also were
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Guanghua, Wen;
Houfu, Deng;
Wenzeng, Bing;
Shunzhong, Luo;
Yanlin, Feng
[1]
- Sichuan Univ., Chengdu (China). West China Hospital, Dept. of Nuclear Medicine
Citation Formats
Guanghua, Wen, Houfu, Deng, Wenzeng, Bing, Shunzhong, Luo, and Yanlin, Feng.
Experimental study of the biological properties of {sup 153}Sm-nano-hydroxyapatite.
China: N. p.,
2005.
Web.
Guanghua, Wen, Houfu, Deng, Wenzeng, Bing, Shunzhong, Luo, & Yanlin, Feng.
Experimental study of the biological properties of {sup 153}Sm-nano-hydroxyapatite.
China.
Guanghua, Wen, Houfu, Deng, Wenzeng, Bing, Shunzhong, Luo, and Yanlin, Feng.
2005.
"Experimental study of the biological properties of {sup 153}Sm-nano-hydroxyapatite."
China.
@misc{etde_20888990,
title = {Experimental study of the biological properties of {sup 153}Sm-nano-hydroxyapatite}
author = {Guanghua, Wen, Houfu, Deng, Wenzeng, Bing, Shunzhong, Luo, and Yanlin, Feng}
abstractNote = {Objective: To investigate a new agent {sup 153}Sm-ethylene diamine tetramethylene phosphonic acid (EDTMP)-nano-Hydroxyapatite (nano-HA) and the in vitro and in vivo performance of three derivatives of this agent aiming to explore a new radiopharmaceutical for targeting therapy of bone metastases. Methods: The nano-HA was synthesized by collosol-gelatum method, and was evaluated by transmission electron microscope (TEM) and X-ray diffraction (XRD). {sup 153}Sm was produced with a high specific activity and excellent radionuclidic purity; adopting independent variable method, that nano-HA being labeled with {sup 153}Sm by EDTMP transfer ligands in the best condition was investigated. The stability of {sup 153}Sm-EDTMP-nano-HA in vitro was studied. {sup 153}Sm-EDTMP-nano-HA was injected into normal rabbits for in vivo study. Both SMMC-7721 and MCF-7 cell lines were divided into three groups, treated with nano-HA, {sup 153}Sm-EDTMP and {sup 153}Sm-EDTMP-nano-HA, respectively, and the survival rate was measured by mono-nuclear cell direct cytotoxicity assay method. Results: The detection with TEM, XRD showed that nano-HA consisted of needle-like microcrystals. The labelling rate of {sup 153}Sm-EDTMP-nano-HA was more than 95%, and was extremely stable in vitro. The scanning of normal rabbits showed that the skeletal system was clear , but other systems, for example, liver, spleen and kidney also were seen. The cell culture experiments in vitro indicated that {sup 153}Sm-EDTMP-nano-HA strongly inhibited the proliferation of SMMC-7721 and MCF-7 cells. The half effective inhibition concentrations of {sup 153}Sm-EDTMP-nano-HA were 1.98 and 0.075 mg/L, respectively; the half effective inhibition concentrations of nano-HA were 3.31 and 0.52 mg/L, respectively; the half effective inhibition concentrations of {sup 153}Sm-EDTMP were 4.32 and 0.67 mg/L, respectively. Conclusion: {sup 153}Sm-EDTMP-nano-HA shows it's fine biological properties, and it is well worth further researching as a promising radiopharmaceutical in nuclide treatment for bone metastases. (authors)}
journal = []
issue = {2}
volume = {25}
place = {China}
year = {2005}
month = {Apr}
}
title = {Experimental study of the biological properties of {sup 153}Sm-nano-hydroxyapatite}
author = {Guanghua, Wen, Houfu, Deng, Wenzeng, Bing, Shunzhong, Luo, and Yanlin, Feng}
abstractNote = {Objective: To investigate a new agent {sup 153}Sm-ethylene diamine tetramethylene phosphonic acid (EDTMP)-nano-Hydroxyapatite (nano-HA) and the in vitro and in vivo performance of three derivatives of this agent aiming to explore a new radiopharmaceutical for targeting therapy of bone metastases. Methods: The nano-HA was synthesized by collosol-gelatum method, and was evaluated by transmission electron microscope (TEM) and X-ray diffraction (XRD). {sup 153}Sm was produced with a high specific activity and excellent radionuclidic purity; adopting independent variable method, that nano-HA being labeled with {sup 153}Sm by EDTMP transfer ligands in the best condition was investigated. The stability of {sup 153}Sm-EDTMP-nano-HA in vitro was studied. {sup 153}Sm-EDTMP-nano-HA was injected into normal rabbits for in vivo study. Both SMMC-7721 and MCF-7 cell lines were divided into three groups, treated with nano-HA, {sup 153}Sm-EDTMP and {sup 153}Sm-EDTMP-nano-HA, respectively, and the survival rate was measured by mono-nuclear cell direct cytotoxicity assay method. Results: The detection with TEM, XRD showed that nano-HA consisted of needle-like microcrystals. The labelling rate of {sup 153}Sm-EDTMP-nano-HA was more than 95%, and was extremely stable in vitro. The scanning of normal rabbits showed that the skeletal system was clear , but other systems, for example, liver, spleen and kidney also were seen. The cell culture experiments in vitro indicated that {sup 153}Sm-EDTMP-nano-HA strongly inhibited the proliferation of SMMC-7721 and MCF-7 cells. The half effective inhibition concentrations of {sup 153}Sm-EDTMP-nano-HA were 1.98 and 0.075 mg/L, respectively; the half effective inhibition concentrations of nano-HA were 3.31 and 0.52 mg/L, respectively; the half effective inhibition concentrations of {sup 153}Sm-EDTMP were 4.32 and 0.67 mg/L, respectively. Conclusion: {sup 153}Sm-EDTMP-nano-HA shows it's fine biological properties, and it is well worth further researching as a promising radiopharmaceutical in nuclide treatment for bone metastases. (authors)}
journal = []
issue = {2}
volume = {25}
place = {China}
year = {2005}
month = {Apr}
}