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Biotin-specific synthetic receptors prepared using molecular imprinting

Abstract

The composition of new molecularly imprinted polymers (MIPs) specific for biotin was optimised using molecular modelling software. Three functional monomers: methacrylic acid (MAA), 2-(trifluoromethyl)acrylic acid (TFAA) and 2-acrylamido-2-methylpropanesulfonic acid (AMPSA), which demonstrated the highest binding scores with biotin, were tested on their ability to generate specific binding sites. The imprinted polymers were photografted to the surface of polystyrene microspheres in water. The affinity of the synthetic 'receptor' sites was evaluated in binding experiments using horseradish peroxidase-labelled biotin. Good correlation was found between the modelling results and the performance of the materials in the template re-binding study. The dissociation constants for all MIPs were 1.4-16.8 nM, which is sufficient for most analytical applications where biotin is used as a label.
Publication Date:
Feb 16, 2004
Product Type:
Journal Article
Resource Relation:
Journal Name: Analytica Chimica Acta; Journal Volume: 504; Journal Issue: 1; Conference: 2. international workshop on molecularly imprinted polymers, Montpellier (France), 15-19 Sep 2002; Other Information: DOI: 10.1016/S0003-2670(03)00813-4; PII: S0003267003008134; Copyright (c) 2003 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; ACRYLIC ACID; AFFINITY; BIOTIN; METHACRYLIC ACID; MICROSPHERES; PERFORMANCE; PEROXIDASES; POLYMERIZATION; POLYSTYRENE; RECEPTORS
OSTI ID:
20874960
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0003-2670; ACACAM; TRN: NL05S3459042483
Submitting Site:
NLN
Size:
page(s) 179-183
Announcement Date:
Jun 15, 2007

Citation Formats

Piletska, Elena, Piletsky, Sergey, Karim, Kal, Terpetschnig, Ewald, and Turner, Anthony. Biotin-specific synthetic receptors prepared using molecular imprinting. Netherlands: N. p., 2004. Web. doi:10.1016/S0003-2670(03)00813-4.
Piletska, Elena, Piletsky, Sergey, Karim, Kal, Terpetschnig, Ewald, & Turner, Anthony. Biotin-specific synthetic receptors prepared using molecular imprinting. Netherlands. doi:10.1016/S0003-2670(03)00813-4.
Piletska, Elena, Piletsky, Sergey, Karim, Kal, Terpetschnig, Ewald, and Turner, Anthony. 2004. "Biotin-specific synthetic receptors prepared using molecular imprinting." Netherlands. doi:10.1016/S0003-2670(03)00813-4. https://www.osti.gov/servlets/purl/10.1016/S0003-2670(03)00813-4.
@misc{etde_20874960,
title = {Biotin-specific synthetic receptors prepared using molecular imprinting}
author = {Piletska, Elena, Piletsky, Sergey, Karim, Kal, Terpetschnig, Ewald, and Turner, Anthony}
abstractNote = {The composition of new molecularly imprinted polymers (MIPs) specific for biotin was optimised using molecular modelling software. Three functional monomers: methacrylic acid (MAA), 2-(trifluoromethyl)acrylic acid (TFAA) and 2-acrylamido-2-methylpropanesulfonic acid (AMPSA), which demonstrated the highest binding scores with biotin, were tested on their ability to generate specific binding sites. The imprinted polymers were photografted to the surface of polystyrene microspheres in water. The affinity of the synthetic 'receptor' sites was evaluated in binding experiments using horseradish peroxidase-labelled biotin. Good correlation was found between the modelling results and the performance of the materials in the template re-binding study. The dissociation constants for all MIPs were 1.4-16.8 nM, which is sufficient for most analytical applications where biotin is used as a label.}
doi = {10.1016/S0003-2670(03)00813-4}
journal = {Analytica Chimica Acta}
issue = {1}
volume = {504}
place = {Netherlands}
year = {2004}
month = {Feb}
}