Abstract
This study is a trial to produce antisera against triiodothyronine (T{sub 3}) which resulted in a poor response of sheep to immunization, since small amount of antibodies were detected, hence the amount of T{sub 3} tracer bound by the antibodies in terms of percentage was found to be less than 10% with weak discrimination between zero and high standard doses in all bleeds which dose not satisfy radioimmunoassay (RIA) technique requirements. In this study, two local males Sudanese sheep (ovis aries) were immunized with T{sub 3}-immuno gen intramuscularly and subcutaneously in different sites along their backs. Sheep (I) was immunized with 25{mu}g of antigen per kg body weight in first and boosting injections, while the dose was only 10{mu}g per kg body weight for sheep(II). T{sub 3}-immuno gen was emulsified in FCA for the first injection and in FIA for the boosting injections. The sera obtained from both sheep after each injection were subjected to evaluation for the levels of circulating anti-T{sub 3} antibodies through both qualitative and quantitative tests (titration test). The tests were performed for both purified and non-purified from with different separation methods, these methods included, precipitation by second antibody assisted by polyethylene glycol (PEG), polystyrene beads,
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Karar, M G. E.
[1]
- Department of Biochemistry, Sudan Academy of Sciences, Atomic Energy Council, Khartoum (Sudan)
Citation Formats
Karar, M G. E.
Production of sheep anti triiodothyronine (T{sub 3}) antisera for development of T{sub 3} RIA kit.
Sudan: N. p.,
2006.
Web.
Karar, M G. E.
Production of sheep anti triiodothyronine (T{sub 3}) antisera for development of T{sub 3} RIA kit.
Sudan.
Karar, M G. E.
2006.
"Production of sheep anti triiodothyronine (T{sub 3}) antisera for development of T{sub 3} RIA kit."
Sudan.
@misc{etde_20873792,
title = {Production of sheep anti triiodothyronine (T{sub 3}) antisera for development of T{sub 3} RIA kit}
author = {Karar, M G. E.}
abstractNote = {This study is a trial to produce antisera against triiodothyronine (T{sub 3}) which resulted in a poor response of sheep to immunization, since small amount of antibodies were detected, hence the amount of T{sub 3} tracer bound by the antibodies in terms of percentage was found to be less than 10% with weak discrimination between zero and high standard doses in all bleeds which dose not satisfy radioimmunoassay (RIA) technique requirements. In this study, two local males Sudanese sheep (ovis aries) were immunized with T{sub 3}-immuno gen intramuscularly and subcutaneously in different sites along their backs. Sheep (I) was immunized with 25{mu}g of antigen per kg body weight in first and boosting injections, while the dose was only 10{mu}g per kg body weight for sheep(II). T{sub 3}-immuno gen was emulsified in FCA for the first injection and in FIA for the boosting injections. The sera obtained from both sheep after each injection were subjected to evaluation for the levels of circulating anti-T{sub 3} antibodies through both qualitative and quantitative tests (titration test). The tests were performed for both purified and non-purified from with different separation methods, these methods included, precipitation by second antibody assisted by polyethylene glycol (PEG), polystyrene beads, and liquid phase separation technique. Tests for the quality and success of polystyrene beads coating process for the titration were done using pre characterized antibodies, namely anti progesterone antibodies and anti thyroxine antibodies. These tests revels that, the coating process including the activation of polystyrene beads, was of good quality, and the results obtained was due to weak response to T{sub 3}-immuno gen. The results obtained from qualitative tests of the two sheep sera did not show clear precipitate, in spite of the positive result obtained in a neat sera, which was an indication for weak antibody formation. The result of the titration tests for all bleeds (third, forth, fifth, sixth, and seventh) of the tow sheep confirmed the result obtained from the qualitative test, although the binding percentages were weak in all bleeds, sheep (II) showed better response than sheep (I). (Author)}
place = {Sudan}
year = {2006}
month = {Nov}
}
title = {Production of sheep anti triiodothyronine (T{sub 3}) antisera for development of T{sub 3} RIA kit}
author = {Karar, M G. E.}
abstractNote = {This study is a trial to produce antisera against triiodothyronine (T{sub 3}) which resulted in a poor response of sheep to immunization, since small amount of antibodies were detected, hence the amount of T{sub 3} tracer bound by the antibodies in terms of percentage was found to be less than 10% with weak discrimination between zero and high standard doses in all bleeds which dose not satisfy radioimmunoassay (RIA) technique requirements. In this study, two local males Sudanese sheep (ovis aries) were immunized with T{sub 3}-immuno gen intramuscularly and subcutaneously in different sites along their backs. Sheep (I) was immunized with 25{mu}g of antigen per kg body weight in first and boosting injections, while the dose was only 10{mu}g per kg body weight for sheep(II). T{sub 3}-immuno gen was emulsified in FCA for the first injection and in FIA for the boosting injections. The sera obtained from both sheep after each injection were subjected to evaluation for the levels of circulating anti-T{sub 3} antibodies through both qualitative and quantitative tests (titration test). The tests were performed for both purified and non-purified from with different separation methods, these methods included, precipitation by second antibody assisted by polyethylene glycol (PEG), polystyrene beads, and liquid phase separation technique. Tests for the quality and success of polystyrene beads coating process for the titration were done using pre characterized antibodies, namely anti progesterone antibodies and anti thyroxine antibodies. These tests revels that, the coating process including the activation of polystyrene beads, was of good quality, and the results obtained was due to weak response to T{sub 3}-immuno gen. The results obtained from qualitative tests of the two sheep sera did not show clear precipitate, in spite of the positive result obtained in a neat sera, which was an indication for weak antibody formation. The result of the titration tests for all bleeds (third, forth, fifth, sixth, and seventh) of the tow sheep confirmed the result obtained from the qualitative test, although the binding percentages were weak in all bleeds, sheep (II) showed better response than sheep (I). (Author)}
place = {Sudan}
year = {2006}
month = {Nov}
}