You need JavaScript to view this

Allele-specific MMP-3 transcription under in vivo conditions

Journal Article:

Abstract

A common matrix metalloproteinases-3 (MMP-3) -1612 5A/6A promoter polymorphism is associated with risk for cardiovascular disease, rheumatoid arthritis, and other diseases. Here we used the haplotype chromatin immunoprecipitation method to study allele-specific MMP-3 expression under in vivo conditions in heterozygous THP-1 cells. Pyrosequencing was used to analyse the ratio of 5A-allele to 6A-allele after chromatin immunoprecipitation using an antibody against phosphorylated active RNA polymerase II. There was no allele-specific difference in transcriptional activity during basal conditions, i.e., in unstimulated monocytic THP-1 cells. However, after stimulation of MMP-3 expression by monocyte differentiation or incubation with IL-1{beta}, the haplotype containing the 5A-allele was associated with higher transcriptional activity compared with the 6A-containing haplotype. Electromobility shift assay demonstrated increased binding of nuclear proteins to the 5A-allele after monocyte differentiation. In conclusion, the common MMP-3 5A/6A promoter polymorphism appears to be functional only during specific environmental conditions involving inflammation.
Authors:
Chaoyong, Zhu; [1]  Odeberg, Jacob; [1]  Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm (Sweden)]; Hamsten, Anders; [1]  Eriksson, Per [1] 
  1. Atherosclerosis Research Unit, King Gustav V Research Institute, Department of Medicine, Karolinska Institute, Stockholm (Sweden)
Publication Date:
Sep 29, 2006
Product Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 348; Journal Issue: 3; Other Information: DOI: 10.1016/j.bbrc.2006.07.174; PII: S0006-291X(06)01752-9; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Subject:
60 APPLIED LIFE SCIENCES; ANTIBODIES; CARDIOVASCULAR DISEASES; CHROMATIN; HAZARDS; IN VIVO; INCUBATION; INFLAMMATION; MONOCYTES; PROMOTERS; PROTEINS; RHEUMATIC DISEASES; RNA; STIMULATION; TRANSCRIPTION
OSTI ID:
20854493
Country of Origin:
United States
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0006-291X; BBRCA9; TRN: US07R0268027483
Submitting Site:
INIS
Size:
page(s) 1150-1156
Announcement Date:
Apr 23, 2007

Journal Article:

Citation Formats

Chaoyong, Zhu, Odeberg, Jacob, Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm (Sweden)], Hamsten, Anders, and Eriksson, Per. Allele-specific MMP-3 transcription under in vivo conditions. United States: N. p., 2006. Web.
Chaoyong, Zhu, Odeberg, Jacob, Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm (Sweden)], Hamsten, Anders, & Eriksson, Per. Allele-specific MMP-3 transcription under in vivo conditions. United States.
Chaoyong, Zhu, Odeberg, Jacob, Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm (Sweden)], Hamsten, Anders, and Eriksson, Per. 2006. "Allele-specific MMP-3 transcription under in vivo conditions." United States.
@misc{etde_20854493,
title = {Allele-specific MMP-3 transcription under in vivo conditions}
author = {Chaoyong, Zhu, Odeberg, Jacob, Department of Biotechnology, AlbaNova University Center, Royal Institute of Technology, Stockholm (Sweden)], Hamsten, Anders, and Eriksson, Per}
abstractNote = {A common matrix metalloproteinases-3 (MMP-3) -1612 5A/6A promoter polymorphism is associated with risk for cardiovascular disease, rheumatoid arthritis, and other diseases. Here we used the haplotype chromatin immunoprecipitation method to study allele-specific MMP-3 expression under in vivo conditions in heterozygous THP-1 cells. Pyrosequencing was used to analyse the ratio of 5A-allele to 6A-allele after chromatin immunoprecipitation using an antibody against phosphorylated active RNA polymerase II. There was no allele-specific difference in transcriptional activity during basal conditions, i.e., in unstimulated monocytic THP-1 cells. However, after stimulation of MMP-3 expression by monocyte differentiation or incubation with IL-1{beta}, the haplotype containing the 5A-allele was associated with higher transcriptional activity compared with the 6A-containing haplotype. Electromobility shift assay demonstrated increased binding of nuclear proteins to the 5A-allele after monocyte differentiation. In conclusion, the common MMP-3 5A/6A promoter polymorphism appears to be functional only during specific environmental conditions involving inflammation.}
journal = {Biochemical and Biophysical Research Communications}
issue = {3}
volume = {348}
place = {United States}
year = {2006}
month = {Sep}
}