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Divergent expression and roles for caveolin-1 in mouse hepatocarcinoma cell lines with varying invasive ability

Abstract

Caveolin-1 is the major component protein of caveolae and associated with a lot of cellular events such as endocytosis, cholesterol homeostasis, signal transduction, and tumorigenesis. The majority of results suggest that caveolin-1 might not only act as a tumor suppressor gene but also a promoting metastasis gene. In this study, the divergent expression and roles of caveolin-1 were investigated in mouse hepatocarcinoma cell lines Hca-F, Hca-P, and Hepa1-6, which have high, low, and no metastatic potential in the lymph nodes, as compared with normal mouse liver cell line IAR-20. The results showed that expression of caveolin-1 mRNA and protein along with the amount of caveolae number in Hca-F cells was higher than that in Hca-P cells, but was not detectable in Hepa1-6 cells. When caveolin-1 expression in Hca-F cells was down-regulated by RNAi approach, Hca-F cells proliferation rate in vitro declined and the expression of lymphangiogenic factor VEGFA in Hca-F decreased as well. Furthermore, in vivo implantation assay indicated that reduction of caveolin-1 expression in Hca-F prevented the lymphatic metastasis tumor burden of Hca-F cells in 615 mice. These results suggest that caveolin-1 facilities the lymphatic metastasis ability of mouse hepatocarcinoma cells via regulation tumor cell growth and VEGFA expression.
Authors:
Huimin, Zhou; [1]  Li, Jia; [1]  Shujing, Wang; [1]  Hongmei, Wang; [1]  Department of Medical Microbiology and Parasitology, School of Medicine, Liaodong College, Dandong 118000 (China)]; Haiying, Chu; [1]  Yichuan, Hu; [1]  Jun, Cao; [1]  Jianing, Zhang [1] 
  1. Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, Dalian 116027 (China)
Publication Date:
Jun 23, 2006
Product Type:
Journal Article
Resource Relation:
Journal Name: Biochemical and Biophysical Research Communications; Journal Volume: 345; Journal Issue: 1; Other Information: DOI: 10.1016/j.bbrc.2006.03.246; PII: S0006-291X(06)00927-2; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL GROWTH; CELL PROLIFERATION; CHOLESTEROL; GENE REGULATION; HOMEOSTASIS; IN VITRO; IN VIVO; LIVER CELLS; LYMPH NODES; METASTASES; MICE; NEOPLASMS; PROTEINS; TUMOR CELLS
OSTI ID:
20854321
Country of Origin:
United States
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0006-291X; BBRCA9; TRN: US07R0096027311
Submitting Site:
INIS
Size:
page(s) 486-494
Announcement Date:
Apr 18, 2007

Citation Formats

Huimin, Zhou, Li, Jia, Shujing, Wang, Hongmei, Wang, Department of Medical Microbiology and Parasitology, School of Medicine, Liaodong College, Dandong 118000 (China)], Haiying, Chu, Yichuan, Hu, Jun, Cao, and Jianing, Zhang. Divergent expression and roles for caveolin-1 in mouse hepatocarcinoma cell lines with varying invasive ability. United States: N. p., 2006. Web. doi:10.1016/j.bbrc.2006.03.246.
Huimin, Zhou, Li, Jia, Shujing, Wang, Hongmei, Wang, Department of Medical Microbiology and Parasitology, School of Medicine, Liaodong College, Dandong 118000 (China)], Haiying, Chu, Yichuan, Hu, Jun, Cao, & Jianing, Zhang. Divergent expression and roles for caveolin-1 in mouse hepatocarcinoma cell lines with varying invasive ability. United States. doi:10.1016/j.bbrc.2006.03.246.
Huimin, Zhou, Li, Jia, Shujing, Wang, Hongmei, Wang, Department of Medical Microbiology and Parasitology, School of Medicine, Liaodong College, Dandong 118000 (China)], Haiying, Chu, Yichuan, Hu, Jun, Cao, and Jianing, Zhang. 2006. "Divergent expression and roles for caveolin-1 in mouse hepatocarcinoma cell lines with varying invasive ability." United States. doi:10.1016/j.bbrc.2006.03.246. https://www.osti.gov/servlets/purl/10.1016/j.bbrc.2006.03.246.
@misc{etde_20854321,
title = {Divergent expression and roles for caveolin-1 in mouse hepatocarcinoma cell lines with varying invasive ability}
author = {Huimin, Zhou, Li, Jia, Shujing, Wang, Hongmei, Wang, Department of Medical Microbiology and Parasitology, School of Medicine, Liaodong College, Dandong 118000 (China)], Haiying, Chu, Yichuan, Hu, Jun, Cao, and Jianing, Zhang}
abstractNote = {Caveolin-1 is the major component protein of caveolae and associated with a lot of cellular events such as endocytosis, cholesterol homeostasis, signal transduction, and tumorigenesis. The majority of results suggest that caveolin-1 might not only act as a tumor suppressor gene but also a promoting metastasis gene. In this study, the divergent expression and roles of caveolin-1 were investigated in mouse hepatocarcinoma cell lines Hca-F, Hca-P, and Hepa1-6, which have high, low, and no metastatic potential in the lymph nodes, as compared with normal mouse liver cell line IAR-20. The results showed that expression of caveolin-1 mRNA and protein along with the amount of caveolae number in Hca-F cells was higher than that in Hca-P cells, but was not detectable in Hepa1-6 cells. When caveolin-1 expression in Hca-F cells was down-regulated by RNAi approach, Hca-F cells proliferation rate in vitro declined and the expression of lymphangiogenic factor VEGFA in Hca-F decreased as well. Furthermore, in vivo implantation assay indicated that reduction of caveolin-1 expression in Hca-F prevented the lymphatic metastasis tumor burden of Hca-F cells in 615 mice. These results suggest that caveolin-1 facilities the lymphatic metastasis ability of mouse hepatocarcinoma cells via regulation tumor cell growth and VEGFA expression.}
doi = {10.1016/j.bbrc.2006.03.246}
journal = {Biochemical and Biophysical Research Communications}
issue = {1}
volume = {345}
place = {United States}
year = {2006}
month = {Jun}
}