Abstract
Caveolin-1 is the major component protein of caveolae and associated with a lot of cellular events such as endocytosis, cholesterol homeostasis, signal transduction, and tumorigenesis. The majority of results suggest that caveolin-1 might not only act as a tumor suppressor gene but also a promoting metastasis gene. In this study, the divergent expression and roles of caveolin-1 were investigated in mouse hepatocarcinoma cell lines Hca-F, Hca-P, and Hepa1-6, which have high, low, and no metastatic potential in the lymph nodes, as compared with normal mouse liver cell line IAR-20. The results showed that expression of caveolin-1 mRNA and protein along with the amount of caveolae number in Hca-F cells was higher than that in Hca-P cells, but was not detectable in Hepa1-6 cells. When caveolin-1 expression in Hca-F cells was down-regulated by RNAi approach, Hca-F cells proliferation rate in vitro declined and the expression of lymphangiogenic factor VEGFA in Hca-F decreased as well. Furthermore, in vivo implantation assay indicated that reduction of caveolin-1 expression in Hca-F prevented the lymphatic metastasis tumor burden of Hca-F cells in 615 mice. These results suggest that caveolin-1 facilities the lymphatic metastasis ability of mouse hepatocarcinoma cells via regulation tumor cell growth and VEGFA expression.
Huimin, Zhou;
[1]
Li, Jia;
[1]
Shujing, Wang;
[1]
Hongmei, Wang;
[1]
Department of Medical Microbiology and Parasitology, School of Medicine, Liaodong College, Dandong 118000 (China)];
Haiying, Chu;
[1]
Yichuan, Hu;
[1]
Jun, Cao;
[1]
Jianing, Zhang
[1]
- Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, Dalian 116027 (China)
Citation Formats
Huimin, Zhou, Li, Jia, Shujing, Wang, Hongmei, Wang, Department of Medical Microbiology and Parasitology, School of Medicine, Liaodong College, Dandong 118000 (China)], Haiying, Chu, Yichuan, Hu, Jun, Cao, and Jianing, Zhang.
Divergent expression and roles for caveolin-1 in mouse hepatocarcinoma cell lines with varying invasive ability.
United States: N. p.,
2006.
Web.
doi:10.1016/j.bbrc.2006.03.246.
Huimin, Zhou, Li, Jia, Shujing, Wang, Hongmei, Wang, Department of Medical Microbiology and Parasitology, School of Medicine, Liaodong College, Dandong 118000 (China)], Haiying, Chu, Yichuan, Hu, Jun, Cao, & Jianing, Zhang.
Divergent expression and roles for caveolin-1 in mouse hepatocarcinoma cell lines with varying invasive ability.
United States.
https://doi.org/10.1016/j.bbrc.2006.03.246
Huimin, Zhou, Li, Jia, Shujing, Wang, Hongmei, Wang, Department of Medical Microbiology and Parasitology, School of Medicine, Liaodong College, Dandong 118000 (China)], Haiying, Chu, Yichuan, Hu, Jun, Cao, and Jianing, Zhang.
2006.
"Divergent expression and roles for caveolin-1 in mouse hepatocarcinoma cell lines with varying invasive ability."
United States.
https://doi.org/10.1016/j.bbrc.2006.03.246.
@misc{etde_20854321,
title = {Divergent expression and roles for caveolin-1 in mouse hepatocarcinoma cell lines with varying invasive ability}
author = {Huimin, Zhou, Li, Jia, Shujing, Wang, Hongmei, Wang, Department of Medical Microbiology and Parasitology, School of Medicine, Liaodong College, Dandong 118000 (China)], Haiying, Chu, Yichuan, Hu, Jun, Cao, and Jianing, Zhang}
abstractNote = {Caveolin-1 is the major component protein of caveolae and associated with a lot of cellular events such as endocytosis, cholesterol homeostasis, signal transduction, and tumorigenesis. The majority of results suggest that caveolin-1 might not only act as a tumor suppressor gene but also a promoting metastasis gene. In this study, the divergent expression and roles of caveolin-1 were investigated in mouse hepatocarcinoma cell lines Hca-F, Hca-P, and Hepa1-6, which have high, low, and no metastatic potential in the lymph nodes, as compared with normal mouse liver cell line IAR-20. The results showed that expression of caveolin-1 mRNA and protein along with the amount of caveolae number in Hca-F cells was higher than that in Hca-P cells, but was not detectable in Hepa1-6 cells. When caveolin-1 expression in Hca-F cells was down-regulated by RNAi approach, Hca-F cells proliferation rate in vitro declined and the expression of lymphangiogenic factor VEGFA in Hca-F decreased as well. Furthermore, in vivo implantation assay indicated that reduction of caveolin-1 expression in Hca-F prevented the lymphatic metastasis tumor burden of Hca-F cells in 615 mice. These results suggest that caveolin-1 facilities the lymphatic metastasis ability of mouse hepatocarcinoma cells via regulation tumor cell growth and VEGFA expression.}
doi = {10.1016/j.bbrc.2006.03.246}
journal = []
issue = {1}
volume = {345}
place = {United States}
year = {2006}
month = {Jun}
}
title = {Divergent expression and roles for caveolin-1 in mouse hepatocarcinoma cell lines with varying invasive ability}
author = {Huimin, Zhou, Li, Jia, Shujing, Wang, Hongmei, Wang, Department of Medical Microbiology and Parasitology, School of Medicine, Liaodong College, Dandong 118000 (China)], Haiying, Chu, Yichuan, Hu, Jun, Cao, and Jianing, Zhang}
abstractNote = {Caveolin-1 is the major component protein of caveolae and associated with a lot of cellular events such as endocytosis, cholesterol homeostasis, signal transduction, and tumorigenesis. The majority of results suggest that caveolin-1 might not only act as a tumor suppressor gene but also a promoting metastasis gene. In this study, the divergent expression and roles of caveolin-1 were investigated in mouse hepatocarcinoma cell lines Hca-F, Hca-P, and Hepa1-6, which have high, low, and no metastatic potential in the lymph nodes, as compared with normal mouse liver cell line IAR-20. The results showed that expression of caveolin-1 mRNA and protein along with the amount of caveolae number in Hca-F cells was higher than that in Hca-P cells, but was not detectable in Hepa1-6 cells. When caveolin-1 expression in Hca-F cells was down-regulated by RNAi approach, Hca-F cells proliferation rate in vitro declined and the expression of lymphangiogenic factor VEGFA in Hca-F decreased as well. Furthermore, in vivo implantation assay indicated that reduction of caveolin-1 expression in Hca-F prevented the lymphatic metastasis tumor burden of Hca-F cells in 615 mice. These results suggest that caveolin-1 facilities the lymphatic metastasis ability of mouse hepatocarcinoma cells via regulation tumor cell growth and VEGFA expression.}
doi = {10.1016/j.bbrc.2006.03.246}
journal = []
issue = {1}
volume = {345}
place = {United States}
year = {2006}
month = {Jun}
}