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Developmental exposure to PBDE 99 and PCB affects estrogen sensitivity of target genes in rat brain regions and female sexual behavior

Abstract

We recently reported effects of PBDE99 (2,2',4,4'5-pentabromoBDE) on sexual differentiation processes in rat reproductive organs and central nervous system. These studies were prompted by reports on an increase of PBDE levels in human milk, an indicator of the body burden of pregnant women and of potential exposure of the nursing infant, during the last decade. Even higher human adipose tissue and milk levels were reported for North America. PBDE99 is present in human and animal samples and exhibits developmental neurotoxicity in mice. The developing brain is subject to the organizing action of estradiol locally formed from circulating testosterone, and thus represents a target for endocrine active chemicals. One molecular mechanism by which chemicals may interfere with sexual brain differentiation, may be a change in the expression of sex hormone (estrogen)-regulated genes. Such effects may manifest themselves in mRNA expression levels, or in the sensitivity of the genes to estrogen. In order to detect alterations of the latter, more subtle parameter, we have conducted experiments in developmentally chemical-exposed rat offspring that were gonadectomized in adulthood and injected with a challenge dose of estradiol. Effects of PBDE99 were compared with those of a commercial PCB mixture, Aroclor 1254, which had previously been  More>>
Authors:
Lichtensteiger, W; Faass, O; Ceccatelli, R; Schlumpf, M [1] 
  1. Zurich Univ. (Switzerland). Inst. of Pharmacology and Toxicology
Publication Date:
Sep 15, 2004
Product Type:
Conference
Report Number:
ETDE-DE-1546
Resource Relation:
Conference: Dioxin 2004: 24. international symposium on halogenated environmental organic pollutants and POPs, Berlin (Germany), 6-10 Sep 2004; Related Information: In: Dioxin 2004: 24. international symposium on halogenated environmental organic pollutants and POPs. Proceedings, Organohalogen Compounds v. 66, 4035 pages.
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; BROMINATED AROMATIC HYDROCARBONS; POLYCHLORINATED BIPHENYLS; ONTOGENESIS; PRENATAL EXPOSURE; RATS; GENE REGULATION; BRAIN; ESTROGENS; BEHAVIOR; FEMALES
Sponsoring Organizations:
Bundesmin. fuer Umwelt, Naturschutz und Reaktorsicherheit, Bonn (Germany)
OSTI ID:
20828440
Research Organizations:
Technische Univ. Berlin (Germany). Inst. fuer Technischen Umweltschutz
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Other: ISBN 3-928379-30-5; TRN: DE07G1079
Availability:
Available as CD-ROM; www.osti.gov/servlets/purl/20828440-TNhKO7/; Commercial reproduction prohibited; OSTI as DE20828440
Submitting Site:
DE
Size:
page(s) 3915-3920
Announcement Date:
Jan 27, 2007

Citation Formats

Lichtensteiger, W, Faass, O, Ceccatelli, R, and Schlumpf, M. Developmental exposure to PBDE 99 and PCB affects estrogen sensitivity of target genes in rat brain regions and female sexual behavior. Germany: N. p., 2004. Web.
Lichtensteiger, W, Faass, O, Ceccatelli, R, & Schlumpf, M. Developmental exposure to PBDE 99 and PCB affects estrogen sensitivity of target genes in rat brain regions and female sexual behavior. Germany.
Lichtensteiger, W, Faass, O, Ceccatelli, R, and Schlumpf, M. 2004. "Developmental exposure to PBDE 99 and PCB affects estrogen sensitivity of target genes in rat brain regions and female sexual behavior." Germany.
@misc{etde_20828440,
title = {Developmental exposure to PBDE 99 and PCB affects estrogen sensitivity of target genes in rat brain regions and female sexual behavior}
author = {Lichtensteiger, W, Faass, O, Ceccatelli, R, and Schlumpf, M}
abstractNote = {We recently reported effects of PBDE99 (2,2',4,4'5-pentabromoBDE) on sexual differentiation processes in rat reproductive organs and central nervous system. These studies were prompted by reports on an increase of PBDE levels in human milk, an indicator of the body burden of pregnant women and of potential exposure of the nursing infant, during the last decade. Even higher human adipose tissue and milk levels were reported for North America. PBDE99 is present in human and animal samples and exhibits developmental neurotoxicity in mice. The developing brain is subject to the organizing action of estradiol locally formed from circulating testosterone, and thus represents a target for endocrine active chemicals. One molecular mechanism by which chemicals may interfere with sexual brain differentiation, may be a change in the expression of sex hormone (estrogen)-regulated genes. Such effects may manifest themselves in mRNA expression levels, or in the sensitivity of the genes to estrogen. In order to detect alterations of the latter, more subtle parameter, we have conducted experiments in developmentally chemical-exposed rat offspring that were gonadectomized in adulthood and injected with a challenge dose of estradiol. Effects of PBDE99 were compared with those of a commercial PCB mixture, Aroclor 1254, which had previously been found to influence sexual brain differentiation. We analyzed the expression of estrogen-regulated genes in ventromedial hypothalamus (VMH) and medial preoptic area (MPO), two brain regions that are part of a network involved in the integration of environmental cues, sexual behavior and gonadal function. Since prominent changes were observed in VMH which is particularly important for female sexual behavior, the study was completed by a behavioral analysis.}
place = {Germany}
year = {2004}
month = {Sep}
}