Abstract
Isopropyl-thio-galactoside (IPTG), an inducer of 3-galactosidase, makes it possible to synthesise this enzyme in vitro with the subcellular structure (P{sub 1}). The enzyme is isolated from the bacteria Escherichia coli K 12 which are inductive but not induced. The incorporation of radioactive amino-acids, which is stimulated by the presence of an inducer, was studied during the course of the enzyme synthesis. Saccharose suppresses the induction of {beta}-galactosidase. The presence of a specific inhibitor in the structure studied is considered. (authors) [French] L'isopropylthiogalactoside (IPTG), inducteur de la 3-galactosidase, permet la synthese in vitro de cette enzyme dans la structure subcellulaire (P{sub 1}) isolee a partir des bacteries d'Escherichia coli K 12, inductibles mais non induites. L'incorporation d'acides amines radioactifs, stimulee par la presence d'inducteur, a ete etudiee au cours de la synthese de l'enzyme. Le saccharose supprime l'induction de la 3-galactosidase. La presence du represseur specifique dans la structure etudiee est consideree. (auteurs)
Nisman, B;
Kayser, A;
Demailly, J;
Genin, C
[1]
- Commissariat a l'Energie Atomique, Saclay (France). Centre d'Etudes Nucleaires
Citation Formats
Nisman, B, Kayser, A, Demailly, J, and Genin, C.
The in vitro synthesis of {beta}-galactosidase induced in a subcellular structure of Escherichia coli (1961); Synthese in vitro de {beta}-galactosidase induite dans une structure subcellulaire d'Escherichia coli (1961).
France: N. p.,
1961.
Web.
Nisman, B, Kayser, A, Demailly, J, & Genin, C.
The in vitro synthesis of {beta}-galactosidase induced in a subcellular structure of Escherichia coli (1961); Synthese in vitro de {beta}-galactosidase induite dans une structure subcellulaire d'Escherichia coli (1961).
France.
Nisman, B, Kayser, A, Demailly, J, and Genin, C.
1961.
"The in vitro synthesis of {beta}-galactosidase induced in a subcellular structure of Escherichia coli (1961); Synthese in vitro de {beta}-galactosidase induite dans une structure subcellulaire d'Escherichia coli (1961)."
France.
@misc{etde_20822446,
title = {The in vitro synthesis of {beta}-galactosidase induced in a subcellular structure of Escherichia coli (1961); Synthese in vitro de {beta}-galactosidase induite dans une structure subcellulaire d'Escherichia coli (1961)}
author = {Nisman, B, Kayser, A, Demailly, J, and Genin, C}
abstractNote = {Isopropyl-thio-galactoside (IPTG), an inducer of 3-galactosidase, makes it possible to synthesise this enzyme in vitro with the subcellular structure (P{sub 1}). The enzyme is isolated from the bacteria Escherichia coli K 12 which are inductive but not induced. The incorporation of radioactive amino-acids, which is stimulated by the presence of an inducer, was studied during the course of the enzyme synthesis. Saccharose suppresses the induction of {beta}-galactosidase. The presence of a specific inhibitor in the structure studied is considered. (authors) [French] L'isopropylthiogalactoside (IPTG), inducteur de la 3-galactosidase, permet la synthese in vitro de cette enzyme dans la structure subcellulaire (P{sub 1}) isolee a partir des bacteries d'Escherichia coli K 12, inductibles mais non induites. L'incorporation d'acides amines radioactifs, stimulee par la presence d'inducteur, a ete etudiee au cours de la synthese de l'enzyme. Le saccharose supprime l'induction de la 3-galactosidase. La presence du represseur specifique dans la structure etudiee est consideree. (auteurs)}
place = {France}
year = {1961}
month = {Jul}
}
title = {The in vitro synthesis of {beta}-galactosidase induced in a subcellular structure of Escherichia coli (1961); Synthese in vitro de {beta}-galactosidase induite dans une structure subcellulaire d'Escherichia coli (1961)}
author = {Nisman, B, Kayser, A, Demailly, J, and Genin, C}
abstractNote = {Isopropyl-thio-galactoside (IPTG), an inducer of 3-galactosidase, makes it possible to synthesise this enzyme in vitro with the subcellular structure (P{sub 1}). The enzyme is isolated from the bacteria Escherichia coli K 12 which are inductive but not induced. The incorporation of radioactive amino-acids, which is stimulated by the presence of an inducer, was studied during the course of the enzyme synthesis. Saccharose suppresses the induction of {beta}-galactosidase. The presence of a specific inhibitor in the structure studied is considered. (authors) [French] L'isopropylthiogalactoside (IPTG), inducteur de la 3-galactosidase, permet la synthese in vitro de cette enzyme dans la structure subcellulaire (P{sub 1}) isolee a partir des bacteries d'Escherichia coli K 12, inductibles mais non induites. L'incorporation d'acides amines radioactifs, stimulee par la presence d'inducteur, a ete etudiee au cours de la synthese de l'enzyme. Le saccharose supprime l'induction de la 3-galactosidase. La presence du represseur specifique dans la structure etudiee est consideree. (auteurs)}
place = {France}
year = {1961}
month = {Jul}
}