Abstract
In the present study, using inhibitors of ceramide synthase (fumonisin B{sub 1}), ketosphinganine synthetase (L-cycloserine), acid sphingomyelinase (D609 and desipramine) and neutral sphingomyelinase (GW4869), the role of ceramide in X-ray-induced apoptosis was investigated in MOLT-4 cells. The diacylglycerol kinase (DGK) assay showed that the intracellular concentration of ceramide increased time-dependently after X irradiation of cells, and this radiation-induced accumulation of ceramide did not occur prior to the appearance of apoptotic cells. Treatment with D609 significantly inhibited radiation-induced apoptosis, but did not inhibit the increase of intracellular ceramide. Treatment with desipramine or GW4869 prevented neither radiation-induced apoptosis nor the induced increase of ceramide. On the other hand, fumonisin B{sub 1} and L-cycloserine had no effect on the radiation-induced induction of apoptosis, in spite of significant inhibition of the radiation-induced ceramide. From these results, it was suggested that the increase of the intracellular concentration of ceramide was not essential for radiation-induced apoptosis in MOLT-4 cells. (author)
Takahashi, Eriko;
Inanami, Osamu;
Asanuma, Taketoshi;
Kuwabara, Mikinori
[1]
- Hokkaido Univ., Graduate School of Veterinary Medicine, Sapporo, Hokkaido (Japan)
Citation Formats
Takahashi, Eriko, Inanami, Osamu, Asanuma, Taketoshi, and Kuwabara, Mikinori.
Effects of ceramide inhibition on radiation-induced apoptosis in human leukemia MOLT-4 cells.
Japan: N. p.,
2006.
Web.
doi:10.1269/jrr.47.19.
Takahashi, Eriko, Inanami, Osamu, Asanuma, Taketoshi, & Kuwabara, Mikinori.
Effects of ceramide inhibition on radiation-induced apoptosis in human leukemia MOLT-4 cells.
Japan.
https://doi.org/10.1269/jrr.47.19
Takahashi, Eriko, Inanami, Osamu, Asanuma, Taketoshi, and Kuwabara, Mikinori.
2006.
"Effects of ceramide inhibition on radiation-induced apoptosis in human leukemia MOLT-4 cells."
Japan.
https://doi.org/10.1269/jrr.47.19.
@misc{etde_20765286,
title = {Effects of ceramide inhibition on radiation-induced apoptosis in human leukemia MOLT-4 cells}
author = {Takahashi, Eriko, Inanami, Osamu, Asanuma, Taketoshi, and Kuwabara, Mikinori}
abstractNote = {In the present study, using inhibitors of ceramide synthase (fumonisin B{sub 1}), ketosphinganine synthetase (L-cycloserine), acid sphingomyelinase (D609 and desipramine) and neutral sphingomyelinase (GW4869), the role of ceramide in X-ray-induced apoptosis was investigated in MOLT-4 cells. The diacylglycerol kinase (DGK) assay showed that the intracellular concentration of ceramide increased time-dependently after X irradiation of cells, and this radiation-induced accumulation of ceramide did not occur prior to the appearance of apoptotic cells. Treatment with D609 significantly inhibited radiation-induced apoptosis, but did not inhibit the increase of intracellular ceramide. Treatment with desipramine or GW4869 prevented neither radiation-induced apoptosis nor the induced increase of ceramide. On the other hand, fumonisin B{sub 1} and L-cycloserine had no effect on the radiation-induced induction of apoptosis, in spite of significant inhibition of the radiation-induced ceramide. From these results, it was suggested that the increase of the intracellular concentration of ceramide was not essential for radiation-induced apoptosis in MOLT-4 cells. (author)}
doi = {10.1269/jrr.47.19}
journal = []
issue = {1}
volume = {47}
place = {Japan}
year = {2006}
month = {Mar}
}
title = {Effects of ceramide inhibition on radiation-induced apoptosis in human leukemia MOLT-4 cells}
author = {Takahashi, Eriko, Inanami, Osamu, Asanuma, Taketoshi, and Kuwabara, Mikinori}
abstractNote = {In the present study, using inhibitors of ceramide synthase (fumonisin B{sub 1}), ketosphinganine synthetase (L-cycloserine), acid sphingomyelinase (D609 and desipramine) and neutral sphingomyelinase (GW4869), the role of ceramide in X-ray-induced apoptosis was investigated in MOLT-4 cells. The diacylglycerol kinase (DGK) assay showed that the intracellular concentration of ceramide increased time-dependently after X irradiation of cells, and this radiation-induced accumulation of ceramide did not occur prior to the appearance of apoptotic cells. Treatment with D609 significantly inhibited radiation-induced apoptosis, but did not inhibit the increase of intracellular ceramide. Treatment with desipramine or GW4869 prevented neither radiation-induced apoptosis nor the induced increase of ceramide. On the other hand, fumonisin B{sub 1} and L-cycloserine had no effect on the radiation-induced induction of apoptosis, in spite of significant inhibition of the radiation-induced ceramide. From these results, it was suggested that the increase of the intracellular concentration of ceramide was not essential for radiation-induced apoptosis in MOLT-4 cells. (author)}
doi = {10.1269/jrr.47.19}
journal = []
issue = {1}
volume = {47}
place = {Japan}
year = {2006}
month = {Mar}
}