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Effects of ceramide inhibition on radiation-induced apoptosis in human leukemia MOLT-4 cells

Journal Article:

Abstract

In the present study, using inhibitors of ceramide synthase (fumonisin B{sub 1}), ketosphinganine synthetase (L-cycloserine), acid sphingomyelinase (D609 and desipramine) and neutral sphingomyelinase (GW4869), the role of ceramide in X-ray-induced apoptosis was investigated in MOLT-4 cells. The diacylglycerol kinase (DGK) assay showed that the intracellular concentration of ceramide increased time-dependently after X irradiation of cells, and this radiation-induced accumulation of ceramide did not occur prior to the appearance of apoptotic cells. Treatment with D609 significantly inhibited radiation-induced apoptosis, but did not inhibit the increase of intracellular ceramide. Treatment with desipramine or GW4869 prevented neither radiation-induced apoptosis nor the induced increase of ceramide. On the other hand, fumonisin B{sub 1} and L-cycloserine had no effect on the radiation-induced induction of apoptosis, in spite of significant inhibition of the radiation-induced ceramide. From these results, it was suggested that the increase of the intracellular concentration of ceramide was not essential for radiation-induced apoptosis in MOLT-4 cells. (author)
Authors:
Takahashi, Eriko; Inanami, Osamu; Asanuma, Taketoshi; Kuwabara, Mikinori [1] 
  1. Hokkaido Univ., Graduate School of Veterinary Medicine, Sapporo, Hokkaido (Japan)
Publication Date:
Mar 15, 2006
Product Type:
Journal Article
Resource Relation:
Journal Name: Journal of Radiation Research; Journal Volume: 47; Journal Issue: 1
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; ANIMAL CELLS; APOPTOSIS; CELL CULTURES; GLYCOLIPIDS; INCUBATION; LEUKEMIA; PHOSPHORUS 32; RADIATION DOSES; RADIATION INJURIES; SPHINGOMYELINS; TIME DEPENDENCE; X RADIATION
OSTI ID:
20765286
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0449-3060; JRARAX; TRN: JP0602364071887
Submitting Site:
INIS
Size:
page(s) 19-25
Announcement Date:
Sep 25, 2006

Journal Article:

Citation Formats

Takahashi, Eriko, Inanami, Osamu, Asanuma, Taketoshi, and Kuwabara, Mikinori. Effects of ceramide inhibition on radiation-induced apoptosis in human leukemia MOLT-4 cells. Japan: N. p., 2006. Web. doi:10.1269/jrr.47.19.
Takahashi, Eriko, Inanami, Osamu, Asanuma, Taketoshi, & Kuwabara, Mikinori. Effects of ceramide inhibition on radiation-induced apoptosis in human leukemia MOLT-4 cells. Japan. doi:10.1269/jrr.47.19.
Takahashi, Eriko, Inanami, Osamu, Asanuma, Taketoshi, and Kuwabara, Mikinori. 2006. "Effects of ceramide inhibition on radiation-induced apoptosis in human leukemia MOLT-4 cells." Japan. doi:10.1269/jrr.47.19. https://www.osti.gov/servlets/purl/10.1269/jrr.47.19.
@misc{etde_20765286,
title = {Effects of ceramide inhibition on radiation-induced apoptosis in human leukemia MOLT-4 cells}
author = {Takahashi, Eriko, Inanami, Osamu, Asanuma, Taketoshi, and Kuwabara, Mikinori}
abstractNote = {In the present study, using inhibitors of ceramide synthase (fumonisin B{sub 1}), ketosphinganine synthetase (L-cycloserine), acid sphingomyelinase (D609 and desipramine) and neutral sphingomyelinase (GW4869), the role of ceramide in X-ray-induced apoptosis was investigated in MOLT-4 cells. The diacylglycerol kinase (DGK) assay showed that the intracellular concentration of ceramide increased time-dependently after X irradiation of cells, and this radiation-induced accumulation of ceramide did not occur prior to the appearance of apoptotic cells. Treatment with D609 significantly inhibited radiation-induced apoptosis, but did not inhibit the increase of intracellular ceramide. Treatment with desipramine or GW4869 prevented neither radiation-induced apoptosis nor the induced increase of ceramide. On the other hand, fumonisin B{sub 1} and L-cycloserine had no effect on the radiation-induced induction of apoptosis, in spite of significant inhibition of the radiation-induced ceramide. From these results, it was suggested that the increase of the intracellular concentration of ceramide was not essential for radiation-induced apoptosis in MOLT-4 cells. (author)}
doi = {10.1269/jrr.47.19}
journal = {Journal of Radiation Research}
issue = {1}
volume = {47}
place = {Japan}
year = {2006}
month = {Mar}
}