You need JavaScript to view this

Effects of IL-6 on proliferation and apoptosis of tumor cells multi-irradiated for tumor-bearing mice

Journal Article:

Abstract

A study was carried out on effects of IL-6 on the proliferation and apoptosis of tumor cells and the expression of apoptosis relevant genes (p53, bcl-2) in tumor cells for three kinds of fractional total-body-irradiated tumor-bearing mice. The apoptotic index, proliferative index, S phase fraction of S{sub 180} sarcoma, H{sub 22} hepatocarcinoma and Lewis lung cancer cells were measured by flowcytometry (FCM) after total-body-irradiation and irradiation plus IL-6. The protein expression level of p53, bcl-2 in three kinds of tumors was also determined by the immunohisto-chemical method (UltraSensitive S-P). The results showed that the S phase fraction and proliferation index in Lewis lung cancer cells were lower in the irradiated plus IL-6 group than in the control, while apoptotic index was higher (P<0.05). However, the experimental results for S{sub 180} sarcoma cells were opposite (P<0.01). In addition, no significant effects were observed in H{sub 22} hepatocarcinoma. These results revealed that IL-6 promoted the apoptosis of irradiated Lewis lung cancer cells (P<0.05), while the apoptosis of S{sub 180} sarcoma (P<0.05) was restrained, and there was no significant effects on the cellular cycle of H{sub 22} hepatocarcinoma (P>0.05). In Lewis lung cancer the expression level of p53 was lower in the IL-6  More>>
Authors:
Yongbiao, Liu; [1]  Xuzhou Medical Univ., Xuzhou (China)]; Side, Yao; [1]  Kai, Mei; Ying, Liu; Jie, Zhao; Xianwen, Zhang; Qiang, Zhou; Xingzhi, Hao [2] 
  1. Chinese Academy of Sciences, Shanghai (China). Shanghai Inst. of Applied Physics
  2. Xuzhou Medical Univ., Xuzhou (China)
Publication Date:
May 15, 2004
Product Type:
Journal Article
Resource Relation:
Journal Name: Nuclear Techniques; Journal Volume: 27; Journal Issue: 5; Other Information: 5 figs., 4 tabs., 17 refs
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; APOPTOSIS; CELL FLOW SYSTEMS; CELL PROLIFERATION; LYMPHOKINES; MICE; TUMOR CELLS; WHOLE-BODY IRRADIATION
OSTI ID:
20763070
Country of Origin:
China
Language:
Chinese
Other Identifying Numbers:
Journal ID: ISSN 0253-3219; NUTEDL; TRN: CN0600472069101
Submitting Site:
INIS
Size:
page(s) 379-384
Announcement Date:
Sep 06, 2006

Journal Article:

Citation Formats

Yongbiao, Liu, Xuzhou Medical Univ., Xuzhou (China)], Side, Yao, Kai, Mei, Ying, Liu, Jie, Zhao, Xianwen, Zhang, Qiang, Zhou, and Xingzhi, Hao. Effects of IL-6 on proliferation and apoptosis of tumor cells multi-irradiated for tumor-bearing mice. China: N. p., 2004. Web.
Yongbiao, Liu, Xuzhou Medical Univ., Xuzhou (China)], Side, Yao, Kai, Mei, Ying, Liu, Jie, Zhao, Xianwen, Zhang, Qiang, Zhou, & Xingzhi, Hao. Effects of IL-6 on proliferation and apoptosis of tumor cells multi-irradiated for tumor-bearing mice. China.
Yongbiao, Liu, Xuzhou Medical Univ., Xuzhou (China)], Side, Yao, Kai, Mei, Ying, Liu, Jie, Zhao, Xianwen, Zhang, Qiang, Zhou, and Xingzhi, Hao. 2004. "Effects of IL-6 on proliferation and apoptosis of tumor cells multi-irradiated for tumor-bearing mice." China.
@misc{etde_20763070,
title = {Effects of IL-6 on proliferation and apoptosis of tumor cells multi-irradiated for tumor-bearing mice}
author = {Yongbiao, Liu, Xuzhou Medical Univ., Xuzhou (China)], Side, Yao, Kai, Mei, Ying, Liu, Jie, Zhao, Xianwen, Zhang, Qiang, Zhou, and Xingzhi, Hao}
abstractNote = {A study was carried out on effects of IL-6 on the proliferation and apoptosis of tumor cells and the expression of apoptosis relevant genes (p53, bcl-2) in tumor cells for three kinds of fractional total-body-irradiated tumor-bearing mice. The apoptotic index, proliferative index, S phase fraction of S{sub 180} sarcoma, H{sub 22} hepatocarcinoma and Lewis lung cancer cells were measured by flowcytometry (FCM) after total-body-irradiation and irradiation plus IL-6. The protein expression level of p53, bcl-2 in three kinds of tumors was also determined by the immunohisto-chemical method (UltraSensitive S-P). The results showed that the S phase fraction and proliferation index in Lewis lung cancer cells were lower in the irradiated plus IL-6 group than in the control, while apoptotic index was higher (P<0.05). However, the experimental results for S{sub 180} sarcoma cells were opposite (P<0.01). In addition, no significant effects were observed in H{sub 22} hepatocarcinoma. These results revealed that IL-6 promoted the apoptosis of irradiated Lewis lung cancer cells (P<0.05), while the apoptosis of S{sub 180} sarcoma (P<0.05) was restrained, and there was no significant effects on the cellular cycle of H{sub 22} hepatocarcinoma (P>0.05). In Lewis lung cancer the expression level of p53 was lower in the IL-6 group and higher in S{sub 180} sarcoma (P<0.05), while unvaried in H{sub 22} hepatocarcinoma as compared with the control (P>0.05). It is considered that tumor cell's proportion in the cellular cycle is changed by IL-6 and the effects of IL-6 on the expression of p53, bcl-2 in different three kinds of tumors are different. IL-6 has radio-sensitive effects on some tumors and opposite effects on other tumors, it may be related to the expression of p53 and bcl-2 in tumor cells. (authors)}
journal = {Nuclear Techniques}
issue = {5}
volume = {27}
place = {China}
year = {2004}
month = {May}
}