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The use of unstable chromosome aberrations and micronuclei in the individual biomonitoring: a comparative study; Emprego das aberracoes cromossomicas instaveis e micronucleos no biomonitoramento individual: estudo comparativo

Abstract

Biodosimetry is based on the investigation of radioinduced biological effects in order to correlate them with the absorbed dose. The quantification of unstable chromosome aberrations and micronuclei, in peripheral blood lymphocytes, are two methods commonly used in biodosimetry. In this context, the aim of this research was to compare these methods in the biomonitoring of health care professionals occupationally exposed to ionizing radiation. In parallel, the technique of C-banding was evaluated for quality control of unstable chromosome aberrations analyses. Thus, samples of peripheral blood from health care professionals of three hospitals from Recife (Brazil) were collected, and the lymphocytes cultures were carried out based on the cytogenetic classical technique. It was pointed out that analysis of micronuclei is faster than the unstable chromosome aberrations ones, which suggests the use of the former in preliminary evaluation in cases of suspected accidental exposure. C-banding technique was efficient, as confirmatory test, in the identification of dicentrics and rings during the analyses of unstable chromosome aberrations, being able to be applied in the quality control in biodosimetry. The comparison between the individual work conditions with the frequencies of unstable aberrations and micronuclei obtained from cytogenetic analysis, resulted in the change of behavior of the  More>>
Publication Date:
Feb 15, 2005
Product Type:
Thesis/Dissertation
Report Number:
INIS-BR-4003
Resource Relation:
Other Information: TH: Dissertacao (M.Sc.); 49 refs., 22 figs., 12 tabs
Subject:
61 RADIATION PROTECTION AND DOSIMETRY; BIOLOGICAL INDICATORS; BIOLOGICAL RADIATION EFFECTS; CHROMOSOMAL ABERRATIONS; DOSE-RESPONSE RELATIONSHIPS; DOSIMETRY; EXPERIMENTAL DATA; LYMPHOCYTES; MEDICAL PERSONNEL; OCCUPATIONAL EXPOSURE; RADIATION DOSES; RADIATION MONITORING; RADIATION PROTECTION
OSTI ID:
20757729
Research Organizations:
Pernambuco Univ., Recife, PE (Brazil). Dept. de Energia Nuclear. Programa de Pos-graduacao em Tecnologias Energeticas e Nucleares
Country of Origin:
Brazil
Language:
Portuguese
Other Identifying Numbers:
TRN: BR0645293064801
Availability:
Available from INIS in electronic form
Submitting Site:
BRN
Size:
84 pages
Announcement Date:
Aug 19, 2006

Citation Formats

Fernandes, Thiago de Salazar e. The use of unstable chromosome aberrations and micronuclei in the individual biomonitoring: a comparative study; Emprego das aberracoes cromossomicas instaveis e micronucleos no biomonitoramento individual: estudo comparativo. Brazil: N. p., 2005. Web.
Fernandes, Thiago de Salazar e. The use of unstable chromosome aberrations and micronuclei in the individual biomonitoring: a comparative study; Emprego das aberracoes cromossomicas instaveis e micronucleos no biomonitoramento individual: estudo comparativo. Brazil.
Fernandes, Thiago de Salazar e. 2005. "The use of unstable chromosome aberrations and micronuclei in the individual biomonitoring: a comparative study; Emprego das aberracoes cromossomicas instaveis e micronucleos no biomonitoramento individual: estudo comparativo." Brazil.
@misc{etde_20757729,
title = {The use of unstable chromosome aberrations and micronuclei in the individual biomonitoring: a comparative study; Emprego das aberracoes cromossomicas instaveis e micronucleos no biomonitoramento individual: estudo comparativo}
author = {Fernandes, Thiago de Salazar e}
abstractNote = {Biodosimetry is based on the investigation of radioinduced biological effects in order to correlate them with the absorbed dose. The quantification of unstable chromosome aberrations and micronuclei, in peripheral blood lymphocytes, are two methods commonly used in biodosimetry. In this context, the aim of this research was to compare these methods in the biomonitoring of health care professionals occupationally exposed to ionizing radiation. In parallel, the technique of C-banding was evaluated for quality control of unstable chromosome aberrations analyses. Thus, samples of peripheral blood from health care professionals of three hospitals from Recife (Brazil) were collected, and the lymphocytes cultures were carried out based on the cytogenetic classical technique. It was pointed out that analysis of micronuclei is faster than the unstable chromosome aberrations ones, which suggests the use of the former in preliminary evaluation in cases of suspected accidental exposure. C-banding technique was efficient, as confirmatory test, in the identification of dicentrics and rings during the analyses of unstable chromosome aberrations, being able to be applied in the quality control in biodosimetry. The comparison between the individual work conditions with the frequencies of unstable aberrations and micronuclei obtained from cytogenetic analysis, resulted in the change of behavior of the professionals involved in this research, with a better observance of the radioprotection standards. (author)}
place = {Brazil}
year = {2005}
month = {Feb}
}