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Quantification of micronuclei in blood lymphocytes of patients exposed to gamma radiation for dose absorbed assessment; Quantificacao de micronucleos em linfocitos de pacientes expostas a radiacao gama para a avaliacao da dose absorvida

Abstract

Dose assessment in an important step to evaluate biological effects as a result of individual exposure to ionizing radiation. The use of cytogenetic dosimetry based on the quantification of micronuclei in lymphocytes is very important to complement physical dosimetry, since the measurement of absorbed dose cannot be always performed. In this research, the quantification of micronuclei was carried out in order to evaluate absorbed dose as a result of radiotherapy with {sup 60}Co, using peripheral blood samples from 5 patients with cervical uterine cancer. For this purpose, an aliquot of whole blood from the individual patients was added in culture medium RPMI 1640 supplemented with fetal calf serum and phytohaemagglutinin. The culture was incubated for 44 hours. Henceforth, cytochalasin B was added to block the dividing lymphocytes in cytokinesis. The culture was returned to the incubator for further of 28 hours. Thus, cells were harvested, processed and analyzed. Values obtained considering micronuclei frequency after pelvis irradiation with absorption of 0,08 Gy and 1,8 Gy were, respectively, 0,0021 and 0,052. These results are in agreement with some recent researches that provided some standard values related to micronuclei frequency induced by gamma radiation exposure in different exposed areas for the human body.  More>>
Publication Date:
Feb 15, 2003
Product Type:
Thesis/Dissertation
Report Number:
INIS-BR-4002
Resource Relation:
Other Information: TH: Dissertacao (M.Sc.); 66 refs., 18 figs., 13 tabs
Subject:
61 RADIATION PROTECTION AND DOSIMETRY; BIOLOGICAL RADIATION EFFECTS; CARCINOMAS; COBALT 60; CYTOLOGICAL TECHNIQUES; DOSE-RESPONSE RELATIONSHIPS; DOSIMETRY; EXPERIMENTAL DATA; GAMMA RADIATION; LYMPHOCYTES; PARTIAL BODY IRRADIATION; PATIENTS; RADIATION DOSES; RADIOTHERAPY; UROGENITAL SYSTEM DISEASES; WHOLE-BODY IRRADIATION
OSTI ID:
20757728
Research Organizations:
Pernambuco Univ., Recife, PE (Brazil). Dept. de Energia Nuclear. Programa de Pos-graduacao em Tecnologias Energeticas e Nucleares
Country of Origin:
Brazil
Language:
Portuguese
Other Identifying Numbers:
TRN: BR0645292064800
Availability:
Available from INIS in electronic form
Submitting Site:
BRN
Size:
91 pages
Announcement Date:
Aug 19, 2006

Citation Formats

Barbosa, Isvania Maria Serafim da Silva. Quantification of micronuclei in blood lymphocytes of patients exposed to gamma radiation for dose absorbed assessment; Quantificacao de micronucleos em linfocitos de pacientes expostas a radiacao gama para a avaliacao da dose absorvida. Brazil: N. p., 2003. Web.
Barbosa, Isvania Maria Serafim da Silva. Quantification of micronuclei in blood lymphocytes of patients exposed to gamma radiation for dose absorbed assessment; Quantificacao de micronucleos em linfocitos de pacientes expostas a radiacao gama para a avaliacao da dose absorvida. Brazil.
Barbosa, Isvania Maria Serafim da Silva. 2003. "Quantification of micronuclei in blood lymphocytes of patients exposed to gamma radiation for dose absorbed assessment; Quantificacao de micronucleos em linfocitos de pacientes expostas a radiacao gama para a avaliacao da dose absorvida." Brazil.
@misc{etde_20757728,
title = {Quantification of micronuclei in blood lymphocytes of patients exposed to gamma radiation for dose absorbed assessment; Quantificacao de micronucleos em linfocitos de pacientes expostas a radiacao gama para a avaliacao da dose absorvida}
author = {Barbosa, Isvania Maria Serafim da Silva}
abstractNote = {Dose assessment in an important step to evaluate biological effects as a result of individual exposure to ionizing radiation. The use of cytogenetic dosimetry based on the quantification of micronuclei in lymphocytes is very important to complement physical dosimetry, since the measurement of absorbed dose cannot be always performed. In this research, the quantification of micronuclei was carried out in order to evaluate absorbed dose as a result of radiotherapy with {sup 60}Co, using peripheral blood samples from 5 patients with cervical uterine cancer. For this purpose, an aliquot of whole blood from the individual patients was added in culture medium RPMI 1640 supplemented with fetal calf serum and phytohaemagglutinin. The culture was incubated for 44 hours. Henceforth, cytochalasin B was added to block the dividing lymphocytes in cytokinesis. The culture was returned to the incubator for further of 28 hours. Thus, cells were harvested, processed and analyzed. Values obtained considering micronuclei frequency after pelvis irradiation with absorption of 0,08 Gy and 1,8 Gy were, respectively, 0,0021 and 0,052. These results are in agreement with some recent researches that provided some standard values related to micronuclei frequency induced by gamma radiation exposure in different exposed areas for the human body. The results presented in this report emphasizes biological dosimetry as an important tool for dose assessment of either total or partial-body exposure to ionizing radiation, mainly in retrospective dose investigation. (author)}
place = {Brazil}
year = {2003}
month = {Feb}
}