You need JavaScript to view this

Metabolic effects of dinoseb, diazinon and esfenvalerate in eyed eggs and alevins of Chinook salmon (Oncorhynchus tshawytscha) determined by {sup 1}H NMR metabolomics

Abstract

Pesticide pulses in the Sacramento River, California, originate from storm-water discharges and non-point source aquatic pollution that can last from a few days to weeks. The Sacramento River and its tributaries have historically supported the majority of California's Chinook salmon (Oncorhynchus tshawytscha) spawning grounds. Three pesticides currently used in the Sacramento Valley - dinoseb, diazinon, and esfenvalerate - were chosen to model the exposure of salmon embryos to storm-water discharges. Static-renewal (96 h) exposures to eyed eggs and alevins resulted in both toxicity and significant changes in metabolism assessed in whole-embryo extracts by {sup 1}H nuclear magnetic resonance (NMR) spectroscopy based metabolomics and HPLC with UV detection (HPLC-UV). The 96-h LC{sub 5} values of eyed eggs and alevins exposed to dinoseb were 335 and 70.6 ppb, respectively, and the corresponding values for diazinon were 545 and 29.5 ppm for eyed eggs and alevins, respectively. The 96-h LC{sub 5} of eyed eggs exposed to esfenvalerate could not be determined due to lack of mortality at the highest exposure concentration, but in alevins was 16.7 ppb. All esfenvalerate exposed alevins developed some degree of lordosis or myoskeletal abnormality and did not respond to stimulus or exhibit normal swimming behavior. ATP concentrations measured  More>>
Authors:
Viant, Mark R; [1]  Pincetich, Christopher A; [2]  Tjeerdema, Ronald S [2] 
  1. School of Biosciences, The University of Birmingham, Edgbaston, Birmingham, B15 2TT (United Kingdom)
  2. Department of Environmental Toxicology, College of Agricultural and Environmental Sciences, University of California, One Shields Avenue, Davis, CA 95616-8588 (United States)
Publication Date:
May 25, 2006
Product Type:
Journal Article
Resource Relation:
Journal Name: Aquatic Toxicology; Journal Volume: 77; Journal Issue: 4; Other Information: DOI: 10.1016/j.aquatox.2006.01.009; PII: S0166-445X(06)00033-6; Copyright (c) 2006 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA)
Subject:
60 APPLIED LIFE SCIENCES; ATP; CALIFORNIA; EGGS; EMBRYOS; HIGGS BOSONS; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; METABOLISM; NUCLEAR MAGNETIC RESONANCE; PESTICIDES; PHOSPHOCREATINE; POINT SOURCES; RIVERS; SALMON; TOXICITY
OSTI ID:
20754459
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0166-445X; AQTODG; TRN: NL06R0487062248
Submitting Site:
NLN
Size:
page(s) 359-371
Announcement Date:
Aug 11, 2006

Citation Formats

Viant, Mark R, Pincetich, Christopher A, and Tjeerdema, Ronald S. Metabolic effects of dinoseb, diazinon and esfenvalerate in eyed eggs and alevins of Chinook salmon (Oncorhynchus tshawytscha) determined by {sup 1}H NMR metabolomics. Netherlands: N. p., 2006. Web. doi:10.1016/j.aquatox.2006.01.009.
Viant, Mark R, Pincetich, Christopher A, & Tjeerdema, Ronald S. Metabolic effects of dinoseb, diazinon and esfenvalerate in eyed eggs and alevins of Chinook salmon (Oncorhynchus tshawytscha) determined by {sup 1}H NMR metabolomics. Netherlands. doi:10.1016/j.aquatox.2006.01.009.
Viant, Mark R, Pincetich, Christopher A, and Tjeerdema, Ronald S. 2006. "Metabolic effects of dinoseb, diazinon and esfenvalerate in eyed eggs and alevins of Chinook salmon (Oncorhynchus tshawytscha) determined by {sup 1}H NMR metabolomics." Netherlands. doi:10.1016/j.aquatox.2006.01.009. https://www.osti.gov/servlets/purl/10.1016/j.aquatox.2006.01.009.
@misc{etde_20754459,
title = {Metabolic effects of dinoseb, diazinon and esfenvalerate in eyed eggs and alevins of Chinook salmon (Oncorhynchus tshawytscha) determined by {sup 1}H NMR metabolomics}
author = {Viant, Mark R, Pincetich, Christopher A, and Tjeerdema, Ronald S}
abstractNote = {Pesticide pulses in the Sacramento River, California, originate from storm-water discharges and non-point source aquatic pollution that can last from a few days to weeks. The Sacramento River and its tributaries have historically supported the majority of California's Chinook salmon (Oncorhynchus tshawytscha) spawning grounds. Three pesticides currently used in the Sacramento Valley - dinoseb, diazinon, and esfenvalerate - were chosen to model the exposure of salmon embryos to storm-water discharges. Static-renewal (96 h) exposures to eyed eggs and alevins resulted in both toxicity and significant changes in metabolism assessed in whole-embryo extracts by {sup 1}H nuclear magnetic resonance (NMR) spectroscopy based metabolomics and HPLC with UV detection (HPLC-UV). The 96-h LC{sub 5} values of eyed eggs and alevins exposed to dinoseb were 335 and 70.6 ppb, respectively, and the corresponding values for diazinon were 545 and 29.5 ppm for eyed eggs and alevins, respectively. The 96-h LC{sub 5} of eyed eggs exposed to esfenvalerate could not be determined due to lack of mortality at the highest exposure concentration, but in alevins was 16.7 ppb. All esfenvalerate exposed alevins developed some degree of lordosis or myoskeletal abnormality and did not respond to stimulus or exhibit normal swimming behavior. ATP concentrations measured by HPLC-UV decreased significantly in eyed eggs due to 250 ppb dinoseb and 10 and 100 ppb esfenvalerate (p < 0.05). Phosphocreatine, as measured by HPLC-UV, decreased significantly in eyed eggs due to 250 ppb dinoseb, 10 and 100 ppb esfenvalerate, and 100 ppm diazinon (p < 0.05). Principal components analyses of {sup 1}H NMR metabolite fingerprints of eyed egg and alevin extracts revealed both dose-dependent and mechanism of action-specific metabolic effects induced by the pesticides. Furthermore, NMR based metabolomics proved to be more sensitive than HPLC-UV in identifying significant changes in sublethal metabolism of pesticide exposed alevins. In conclusion, we have demonstrated several benefits of a metabolomics approach for chemical risk assessment, when used in conjunction with a fish embryo assay, and have identified significant metabolic perturbations to the early life stages of Chinook salmon by currently used pesticides.}
doi = {10.1016/j.aquatox.2006.01.009}
journal = {Aquatic Toxicology}
issue = {4}
volume = {77}
place = {Netherlands}
year = {2006}
month = {May}
}