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Induced mutation In Nelumbo nucifera Gaertn. by gamma irradiation

Abstract

Lotus (Nelumbo nucifera Gaertn.) seeds were exposed to different levels of Gamma irradiation at 0, 2, 4, 6, 8 and 10 Kilo rad. The exposed embryos were cultured on solid M S medium supplemented with 2 mg/l B A and overlaid with a liquid M S medium without growth regulators for 1 month. It was found that 50% growth reduction (GR50) was at 6 Kilo rad. The plant lets were subcultured twice every 2 months then they were transferred to solid M S medium supplemented with 4 mg/l NAA and overlaid with M S medium without growth regulators for root induction. The results revealed that plant lets in the control treatment (not exposed to Gamma irradiation) had the highest shoot growth and adventitious root formation. Plant lets at 2-Kilo rad treatment showed the second highest growth. The plant lets at 4-Kilo rad treatment did not exhibit adventitious root formation and had abnormal characteristics. Treatments at 6-10 Kilo rad inhibited the growth of the plant lets.
Authors:
Lama, Sainiya; Aeksomtramaet, Ladda; Kanchanapoom, Kamnoon [1] 
  1. Department of Biology, Faculty of Science, Prince of Songkla University, Songkhla (Thailand)
Publication Date:
Oct 15, 2005
Product Type:
Conference
Report Number:
INIS-TH-100
Resource Relation:
Conference: 31. Congress on Science and Technology of Thailand, Nakhon Ratchasima (Thailand), 18-20 Oct 2005
Subject:
60 APPLIED LIFE SCIENCES; EXPERIMENTAL DATA; FLOWERS; GAMMA SPECTROSCOPY; IRRADIATION; MUTATIONS; PLANT BREEDING; SEEDS
OSTI ID:
20745696
Country of Origin:
Thailand
Language:
Thai
Other Identifying Numbers:
TRN: TH0600014055014
Availability:
Available from INIS in electronic form; Also available from The Science Society of Thailand under the Patronage of His Majesty the King, Bangkok (TH)
Submitting Site:
INIS
Size:
3 pages
Announcement Date:
Jul 31, 2006

Citation Formats

Lama, Sainiya, Aeksomtramaet, Ladda, and Kanchanapoom, Kamnoon. Induced mutation In Nelumbo nucifera Gaertn. by gamma irradiation. Thailand: N. p., 2005. Web.
Lama, Sainiya, Aeksomtramaet, Ladda, & Kanchanapoom, Kamnoon. Induced mutation In Nelumbo nucifera Gaertn. by gamma irradiation. Thailand.
Lama, Sainiya, Aeksomtramaet, Ladda, and Kanchanapoom, Kamnoon. 2005. "Induced mutation In Nelumbo nucifera Gaertn. by gamma irradiation." Thailand.
@misc{etde_20745696,
title = {Induced mutation In Nelumbo nucifera Gaertn. by gamma irradiation}
author = {Lama, Sainiya, Aeksomtramaet, Ladda, and Kanchanapoom, Kamnoon}
abstractNote = {Lotus (Nelumbo nucifera Gaertn.) seeds were exposed to different levels of Gamma irradiation at 0, 2, 4, 6, 8 and 10 Kilo rad. The exposed embryos were cultured on solid M S medium supplemented with 2 mg/l B A and overlaid with a liquid M S medium without growth regulators for 1 month. It was found that 50% growth reduction (GR50) was at 6 Kilo rad. The plant lets were subcultured twice every 2 months then they were transferred to solid M S medium supplemented with 4 mg/l NAA and overlaid with M S medium without growth regulators for root induction. The results revealed that plant lets in the control treatment (not exposed to Gamma irradiation) had the highest shoot growth and adventitious root formation. Plant lets at 2-Kilo rad treatment showed the second highest growth. The plant lets at 4-Kilo rad treatment did not exhibit adventitious root formation and had abnormal characteristics. Treatments at 6-10 Kilo rad inhibited the growth of the plant lets.}
place = {Thailand}
year = {2005}
month = {Oct}
}