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Autoradiographic methods for studying marked volatile substances (1961); Methode.d'etude autoradiographique de substances marquees volatiles (1961)

Abstract

The autoradiographic methods for animals used up to the present do not make it possible to localise exactly the distribution of marked volatile molecules. The Ullberg method (1954) which we have modified (Cohen, Delassue, 1959) involves cold desiccant. The method due to Pellerin (1957) avoids this desiccant but the histological comparison of the autoradiography with the biological document itself is difficult, if not impossible. Nevertheless, we have adopted certain points in the two methods and propose the following technique for the autoradiographic study of marked volatile molecules: 1- The surface of the frozen sample to be studied is prepared using a freezing microtome. 2- The last section, which is 20 {mu} thick and whose histological elements are parallel to those of the block, is dried by cooling and is used as the biological reference document for the autoradiography obtained, as is indicated in 3; 3- The radiography films are applied to the frozen block at -30 deg. C. The autoradiographs correspond to the radioactivity of the volatile molecule and of its non-volatile degradation products. 4- The radiographic film is also applied to the 20 {mu} section previously dried at -20 deg. C. This autoradiography corresponds to the radioactivity of the  More>>
Authors:
Cohen, Y; Wepierre, J [1] 
  1. Commissariat a l'Energie Atomique, Saclay (France). Centre d'Etudes Nucleaires
Publication Date:
Jul 01, 1961
Product Type:
Technical Report
Report Number:
CEA-R-2071
Resource Relation:
Conference: 1. European Autoradiography symposium for medical science, Communication presentee au premier symposium Europeen d'autoradiographie dans les sciences medicales, Rome (Italy), 17-18 Jun 1961; Other Information: 6 refs
Subject:
60 APPLIED LIFE SCIENCES; ANIMAL TISSUES; AUTORADIOGRAPHY; CARBON 14 COMPOUNDS; HISTOLOGICAL TECHNIQUES; HISTOLOGY; LYOPHILIZATION; VOLATILE MATTER
OSTI ID:
20740200
Research Organizations:
CEA Saclay, 91 - Gif-sur-Yvette (France)
Country of Origin:
France
Language:
French
Other Identifying Numbers:
TRN: FR06R2071050016
Availability:
Available from INIS in electronic form
Submitting Site:
FRN
Size:
19 pages
Announcement Date:
Jun 16, 2006

Citation Formats

Cohen, Y, and Wepierre, J. Autoradiographic methods for studying marked volatile substances (1961); Methode.d'etude autoradiographique de substances marquees volatiles (1961). France: N. p., 1961. Web.
Cohen, Y, & Wepierre, J. Autoradiographic methods for studying marked volatile substances (1961); Methode.d'etude autoradiographique de substances marquees volatiles (1961). France.
Cohen, Y, and Wepierre, J. 1961. "Autoradiographic methods for studying marked volatile substances (1961); Methode.d'etude autoradiographique de substances marquees volatiles (1961)." France.
@misc{etde_20740200,
title = {Autoradiographic methods for studying marked volatile substances (1961); Methode.d'etude autoradiographique de substances marquees volatiles (1961)}
author = {Cohen, Y, and Wepierre, J}
abstractNote = {The autoradiographic methods for animals used up to the present do not make it possible to localise exactly the distribution of marked volatile molecules. The Ullberg method (1954) which we have modified (Cohen, Delassue, 1959) involves cold desiccant. The method due to Pellerin (1957) avoids this desiccant but the histological comparison of the autoradiography with the biological document itself is difficult, if not impossible. Nevertheless, we have adopted certain points in the two methods and propose the following technique for the autoradiographic study of marked volatile molecules: 1- The surface of the frozen sample to be studied is prepared using a freezing microtome. 2- The last section, which is 20 {mu} thick and whose histological elements are parallel to those of the block, is dried by cooling and is used as the biological reference document for the autoradiography obtained, as is indicated in 3; 3- The radiography films are applied to the frozen block at -30 deg. C. The autoradiographs correspond to the radioactivity of the volatile molecule and of its non-volatile degradation products. 4- The radiographic film is also applied to the 20 {mu} section previously dried at -20 deg. C. This autoradiography corresponds to the radioactivity of the non-volatile degradation products of the molecule. 5- We confirmed the absence of diffusion of the volatile molecule and of pseudo-radiographic effects (photochemical and others). This method, which has enabled us to study the distribution of a carbide, para-cymene (C{sup 14}) 7, macroscopically in the case of a whole mouse and microscopically on the skin of a dog, can find general applications. (authors) [French] Les methodes d'autoradiographies sur l'animal, proposees jusqu'a present, ne permettent pas de localiser de facon precise la distribution de molecules marquees volatiles. En effet, la methode de Ullberg (1954) que nous avons modifiee (Cohen, Delassue, 1959) necessite la dessiccation par le froid. La methode de Pellerin (1957) evite cette dessication, mais la comparaison a l'echelle histologique de l'autoradiographie avec le document biologique lui-meme est difficile sinon impossible. Cependant, nous nous sommes inspire de certains points de chacune des deux methodes pour proposer la technique suivante d'etude autoradiographique de molecules marquees volatiles: 1- nous preparons la surface du bloc congele a autoradiographier a l'aide d'un microtome a congelation; 2- la derniere coupe de 20 {mu} d'epaisseur, dont les elements histologiques font face a ceux du bloc, est dessechee au froid et sert de document biologique de reference a l'autoradiographie obtenue, comme il est indique en 3; 3- les films radiographiques sont appliques sur le bloc congele a -30 deg. C. Les autoradiographies correspondent a la radioactivite de la molecule volatile et de ses produits de degradation non volatils. 4- De plus, le film radiographique est applique sur la coupe de 20 {mu} prealablement dessechee a -20 deg. C. Cette autoradiographie correspond a la radioactivite des produits de degradation non volatils de la molecule. 5- Nous nous sommes assuree de l'absence de diffusion de la molecule volatile et d'effets pseudo-radiographiques (photochimiques et autres). Cette methode qui nous a permis d'etudier la distribution d'un carbure, le paracymene ({sup 14}C) 7 a l'echelle macroscopique sur la souris entiere et a l'echelle microscopique sur la peau de chien, est susceptible d'une application generale. (auteurs)}
place = {France}
year = {1961}
month = {Jul}
}